| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,145,972 | G→A | D37N (GAT→AAT) | rpoD → | RNA polymerase sigma factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,145,972 | 0 | G | A | 95.9% | 181.0 / ‑0.4 | 49 | D37N (GAT→AAT) | rpoD | RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (27/20); total (28/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
TGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGAT > REL606/3145899‑3146046 | tGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAAt > 1:888474/1‑76 (MQ=255) gTCACCCGTGGTAAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAAtt > 1:704472/1‑76 (MQ=255) aaGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCg > 2:161656/1‑76 (MQ=255) aGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGa < 1:317635/76‑1 (MQ=255) gAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAg < 1:237748/76‑1 (MQ=255) aaGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGAcat > 1:765971/1‑76 (MQ=255) aaGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGAcat > 1:1415424/1‑76 (MQ=255) aaGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGAcat > 1:1449552/1‑76 (MQ=255) aaGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGAcat > 1:310638/1‑76 (MQ=255) aGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGAcatc < 1:1337490/76‑1 (MQ=255) gCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGAcatcat > 1:1648166/1‑76 (MQ=255) cTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACatcatc > 2:137060/1‑76 (MQ=255) cTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACatcatc > 2:1431880/1‑76 (MQ=255) aTCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCa > 2:1172134/1‑76 (MQ=255) aTCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCa < 1:1109370/76‑1 (MQ=255) tCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCaa < 1:353563/76‑1 (MQ=255) gACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATg > 2:1252150/1‑76 (MQ=255) gACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATg > 2:148237/1‑76 (MQ=255) gACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATg > 2:687137/1‑76 (MQ=255) aCCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGCTTCAGATCAGATCGAAGACATCATCCAAATGa > 2:96539/1‑76 (MQ=255) ccTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGAt > 1:796175/1‑76 (MQ=255) tGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAAc > 2:63290/1‑76 (MQ=255) cGAGGTCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGAc < 1:952572/76‑1 (MQ=255) tCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATggg > 1:746387/1‑76 (MQ=255) tCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATggg < 1:1025636/76‑1 (MQ=255) tCAATGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATggg < 1:1239840/76‑1 (MQ=255) aaTGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCa < 2:948070/76‑1 (MQ=255) aaTGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCa > 2:1446569/1‑76 (MQ=255) aaTGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCa > 2:132948/1‑76 (MQ=255) aTGACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCAt > 2:48652/1‑76 (MQ=255) gACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTc < 1:1606571/76‑1 (MQ=255) gACCATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTc < 2:917072/76‑1 (MQ=255) cATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAgg > 2:1161884/1‑76 (MQ=255) cATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAgg > 2:586071/1‑76 (MQ=255) cATCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAgg > 2:1326520/1‑76 (MQ=255) cATCTGCCGGAAGATATCCTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAgg > 2:672174/1‑76 (MQ=255) aTCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGt > 2:605829/1‑76 (MQ=255) tCTGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTg < 2:23/76‑1 (MQ=255) tGCCGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGAt < 1:1106174/76‑1 (MQ=255) ccGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATgg < 1:1066331/76‑1 (MQ=255) cGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGga < 1:1596510/76‑1 (MQ=255) cGGAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGga < 1:829826/76‑1 (MQ=255) ggAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGgaa > 1:185451/1‑76 (MQ=255) ggAAGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGgaa < 1:760337/76‑1 (MQ=255) aaGATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGgaaga < 2:1094064/76‑1 (MQ=255) gATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGaagaag < 1:457787/76‑1 (MQ=255) gATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGaagaag < 1:1086418/76‑1 (MQ=255) gATATCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGaagaag < 1:462045/76‑1 (MQ=255) tatCGTCAATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCa > 2:1410276/1‑76 (MQ=255) cgtcaATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCg > 2:1293821/1‑76 (MQ=255) cgtcaATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCg > 2:1214660/1‑76 (MQ=255) cgtcaATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCg > 1:30318/1‑76 (MQ=255) cgtcaATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCg > 2:616262/1‑76 (MQ=255) gtcgATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCgg < 1:251279/76‑1 (MQ=255) cgATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGAt < 2:194729/76‑1 (MQ=255) cgATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGAt < 2:1048463/76‑1 (MQ=255) | TGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGAT > REL606/3145899‑3146046 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |