| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,202,529 | +CT | coding (31/825 nt) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,202,526 | 1 | . | T | 89.3% | 99.7 / 5.8 | 28 | coding (34/825 nt) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base T (0/25); total (1/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01 | |||||||||||
| * | REL606 | 4,202,526 | 2 | . | C | 89.3% | 102.7 / 6.6 | 28 | coding (34/825 nt) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (0/25); total (1/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01 | |||||||||||
TCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTT‑‑TCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGA > REL606/4202456‑4202586 || tCCAGTAATTTCAGGCCGCGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAGCGGCGGG‑‑TTT‑‑TCtct < 1:836885/76‑3 (MQ=255)tCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTT‑‑TCtct < 2:8403/76‑3 (MQ=255) ccAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTT‑‑TCtctg < 2:72382/76‑4 (MQ=255) gTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑‑TCTGCcg > 1:1457135/1‑76 (MQ=255) tCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTc < 1:1547618/76‑1 (MQ=255) tCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTc < 2:1164655/76‑1 (MQ=255) ccACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCggg < 2:1337958/76‑1 (MQ=255) cgcgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAAt < 2:454434/76‑1 (MQ=255) gcgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATg < 2:1600675/76‑1 (MQ=255) gTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑GTT‑‑TCTTCCGCGGTGCGCGGGGGTggggg > 1:309185/1‑74 (MQ=255) ttAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑‑TCTGCCGCGCTTCGCGGGGGTgggg > 1:735403/1‑75 (MQ=255) tAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑‑TCTTCCGCGGTTCGCGGGGATggggg > 2:680597/1‑74 (MQ=255) tAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTg < 1:81267/76‑1 (MQ=255) aaaGACTGACCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGc < 2:435899/76‑1 (MQ=255) aaaGACTGAACCTGTCCAGTCGCTGGTTCGGTGGCGACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGc < 2:299359/76‑1 (MQ=255) aaaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑‑TCTGCCGCGTTTCGCGGGGATgggggg > 2:1359977/1‑73 (MQ=255) aaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCg < 1:1609624/76‑1 (MQ=255) aGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCGa < 2:335662/76‑1 (MQ=255) aCTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCGAcc < 2:9388/76‑1 (MQ=255) tGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTT‑‑TCTGCCGCGGTTCGCGGGGATGGGGGGGGCCAt > 2:1644937/1‑76 (MQ=255) gAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATg < 2:111435/76‑1 (MQ=255) aaCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCTGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGa < 2:243988/76‑1 (MQ=255) aaCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGa < 2:1155798/76‑1 (MQ=255) aCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGAc < 2:1299892/76‑1 (MQ=255) tGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑‑TCTGCCGCGTTTCGCGGGGATGGGGGGGGCCATGACAgg > 2:600957/1‑75 (MQ=255) gTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCtt < 2:1253227/76‑1 (MQ=255) gTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCtt < 1:1457127/76‑1 (MQ=255) cGCTGGTGCGGTGGCAACGGCGGGGTTTT‑‑TCTGCCGCGGTTCGCGGGGGTGGGGGGGAGCATGAGCGTCTTCtttt > 2:862721/1‑76 (MQ=255) tGGTGCGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCt < 2:1634338/76‑1 (MQ=255) cGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATc < 1:1446263/76‑1 (MQ=255) cGGTGGCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATc < 1:1594726/76‑1 (MQ=255) ggCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTgg < 2:334154/76‑1 (MQ=255) ggCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTgg < 2:1150611/76‑1 (MQ=255) ggCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTgg < 1:284341/76‑1 (MQ=255) gCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGa < 2:1125821/76‑1 (MQ=255) gCAACGGCGGG‑‑TTTTCTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGa < 1:696868/76‑1 (MQ=255) || TCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGG‑‑TTT‑‑TCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGA > REL606/4202456‑4202586 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |