Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	474,392	Δ216 bp	coding (699‑914/1677 nt)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	474392	474607	216	45 [1]	[1] 45	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 474391	1 (0.020)	40 (0.840)	36/136	0.3	97.7%	coding (915/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
	?	REL606	474608 =	1 (0.020)					coding (698/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

AGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAAC  >  REL606/474318‑474466                                                                          |                                                                            aGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgc                                                                           >  1:742884‑M1/1‑74 (MQ=255)    gACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccag                                                                        <  2:744401‑M1/76‑6 (MQ=255)    gACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccag                                                                        >  1:287889‑M1/1‑71 (MQ=255)     aCCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagc                                                                       <  2:203129‑M1/76‑7 (MQ=255)        aaaCAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcacc                                                                    <  1:1125207‑M1/76‑10 (MQ=255)             gTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacag                                                               <  1:1551223‑M1/76‑15 (MQ=255)              acgCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAAAAATCGCCAGCGTCGCCAGCgccagcaccgacagg                                                              <  1:454854‑M1/75‑16 (MQ=255)                 cACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggta                                                           <  2:526050‑M1/76‑19 (MQ=255)                  aCCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggtaa                                                          <  1:449977‑M1/76‑20 (MQ=255)                   ccAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggtaaa                                                         <  1:319609‑M1/76‑21 (MQ=255)                   ccAAGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggtaaa                                                         <  2:1591821‑M1/76‑21 (MQ=255)                     aGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggtaaaca                                                       <  2:1148686‑M1/76‑23 (MQ=255)                            aTGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcg                                                <  2:1602759‑M1/76‑30 (MQ=255)                               cGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgaga                                             >  2:936381‑M1/1‑44 (MQ=255)                                 gCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgagaac                                           >  1:989006‑M1/1‑42 (MQ=255)                                  cTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggcaaacagctcgcgagaacc                                          >  1:977473‑M1/1‑41 (MQ=255)                                    aaCGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgagaaccgg                                        >  2:73778‑M1/1‑39 (MQ=255)                                    aaCGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgagaaccgg                                        >  2:1233358‑M1/1‑39 (MQ=255)                                        aCTTACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccaacagggtaaacagctcgcgagaaccggttgc                                    >  1:285810‑M1/1‑35 (MQ=255)                                          ttACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgagaaccggttgccg                                  >  2:1324007‑M1/1‑33 (MQ=255)                                           tACCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgagaaccggttgccgc                                 >  2:1237246‑M1/1‑32 (MQ=255)                                            aCCAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcg                                <  2:1245560‑M1/76‑46 (MQ=255)                                              cAAACAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgct                              >  1:1060319‑M1/1‑29 (MQ=255)                                               aaaCAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctg                             <  2:1553210‑M1/76‑49 (MQ=255)                                               aaaCAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCg                             <  2:1106695/76‑1 (MQ=255)                                                aaCAGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgc                            >  2:785602‑M1/1‑27 (MQ=255)                                                   aGAATAATCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtg                         >  1:1578822‑M1/1‑24 (MQ=255)                                                     aataatCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgcc                       >  1:621674‑M1/1‑22 (MQ=255)                                                       taatCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccat                     <  1:594321‑M1/76‑57 (MQ=255)                                                        aatCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccata                    >  2:1046297‑M1/1‑19 (MQ=255)                                                        aatCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccata                    >  1:1451876‑M1/1‑19 (MQ=255)                                                        aatCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccata                    >  2:24559‑M1/1‑19 (MQ=255)                                                        aatCGCCAGCGTCGCCAGCgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccata                    <  2:287334‑M1/76‑58 (MQ=255)                                                          tcgccagcgtcgccagcgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccataat                  <  1:91115‑M1/76‑60 (MQ=255)                                                           cgcctgcgtcgccagcgccagccccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccataatc                 <  2:1010430‑M1/76‑61 (MQ=255)                                                            gccagcgtcgccagcgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccataatcc                >  2:208711‑M1/1‑15 (MQ=255)                                                            gccagcgtcgccagcgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccataatcc                <  1:1133701‑M1/76‑62 (MQ=255)                                                              cagcgtcgccagcgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccataatccac              >  1:1375325‑M1/1‑13 (MQ=255)                                                                 cgtcgccagcgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccataatccacggc           <  1:371901‑M1/76‑67 (MQ=255)                                                                  gtcgccagcgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccataatccacggca          >  1:932977‑M1/1‑9 (MQ=255)                                                                  gtcgccagcgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccataatccacggca          <  1:538555‑M1/76‑68 (MQ=255)                                                                    cgccagcgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccataatccacggcacc        <  1:428947‑M1/76‑70 (MQ=255)                                                                       cagcgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccataatccacggcaccaga     >  1:1120357‑M1/1‑4 (MQ=255)                                                                        agcgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccataatccacggcaccagac    >  2:1305205‑M1/1‑3 (MQ=255)                                                                          cgccagcaccgacagggtaaacagctcgcgagaaccggttgccgcgctgcgtgccataatccacggcaccagacgg  <  1:1292103‑M1/76‑76 (MQ=255)                                                                          |                                                                            AGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAAC  >  REL606/474318‑474466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑