| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 474,210 | Δ1 bp | coding (1096/1677 nt) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 474,210 | 0 | G | . | 96.4% | 121.5 / ‑0.0 | 28 | coding (1096/1677 nt) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (13/14); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.47e-01 | |||||||||||
CCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGC > REL606/474144‑474282 | ccAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGc > 1:209876/1‑76 (MQ=255) agtagtGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGGTTT‑TCCGGCCCGCgg > 2:1402843/1‑76 (MQ=255) agtaggGCGAACAGTACGGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCgg < 1:468713/76‑1 (MQ=255) agtGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAg < 1:1121518/76‑1 (MQ=255) agtGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAg > 2:1715758/1‑76 (MQ=255) gtGCGAACAGTACCGGGTGGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGt < 1:487832/76‑1 (MQ=255) gCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTa < 1:1588874/76‑1 (MQ=255) cGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTaaa < 1:1737909/76‑1 (MQ=255) gAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAAt > 2:221564/1‑76 (MQ=255) aCAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTc > 1:332062/1‑76 (MQ=255) cAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCa < 1:187889/76‑1 (MQ=255) aGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCaa > 1:1788708/1‑76 (MQ=255) aCCGGGTTGAGCATAATCGACAGGATCGCCCCCGCCAGTACCAGGCTTT‑TCCGGCCTCCCGCAGGAAACACAAtac > 2:1479318/1‑74 (MQ=255) cggggTTGAGCATACTCGACAGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCAATGcc < 1:717981/74‑1 (MQ=255) ccGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCAATGcc < 1:1499670/76‑1 (MQ=255) cGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCAATGCCa > 2:493956/1‑76 (MQ=255) cGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCAATGCCa > 2:847788/1‑76 (MQ=255) gAGCATAGGCGTCAGGATCCCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCa < 1:113133/76‑1 (MQ=255) cATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTc > 1:1295215/1‑76 (MQ=255) tAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTccc < 1:15345/76‑1 (MQ=255) ccGACAGGAGCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGcc < 1:739271/75‑1 (MQ=255) aCAGGATCCCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAgg < 1:941778/76‑1 (MQ=255) aGGATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGAt > 2:23280/1‑76 (MQ=255) ggATCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATa < 1:1754352/76‑1 (MQ=255) aTCGCCCCTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATaaa < 1:987067/76‑1 (MQ=255) ccccTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAAcgcg < 1:1193102/76‑1 (MQ=255) cTGCCAGTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAAc > 1:1393954/1‑76 (MQ=255) gTACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCa > 2:52670/1‑76 (MQ=255) tACCAGGTTTT‑TCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCaa > 1:19701/1‑76 (MQ=255) ttttTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTgcgc > 2:599217/1‑76 (MQ=255) | CCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGC > REL606/474144‑474282 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |