| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,141,329 | Δ1 bp | intergenic (‑84/+110) | ECB_02035 ← / ← ECB_02036 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,141,329 | 0 | A | . | 96.3% | 246.9 / 3.7 | 54 | intergenic (‑84/+110) | ECB_02035/ECB_02036 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (19/33); total (20/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
TCTTTGCTAAGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTGATCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCGTGAGATTATTT > REL606/2141254‑2141396 | tctTTGCTAAGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTGa > 2:10112/1‑76 (MQ=255) tGCTAAGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAAc > 1:1500255/1‑76 (MQ=255) gCTAAGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACg > 1:1783168/1‑76 (MQ=255) gCTAAGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACg < 1:1098212/76‑1 (MQ=255) cTAAGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTGATCAACg > 2:1650530/1‑76 (MQ=255) aaGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCt > 2:972471/1‑76 (MQ=255) aaGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCt > 2:197825/1‑76 (MQ=255) aaGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCt > 2:215454/1‑76 (MQ=255) aGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTc > 2:263224/1‑76 (MQ=255) cGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAg < 1:1376000/76‑1 (MQ=255) gCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGt < 1:1192622/76‑1 (MQ=255) tGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTAt < 1:453818/76‑1 (MQ=255) gTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATc > 1:1003255/1‑76 (MQ=255) gTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATc > 2:1761366/1‑76 (MQ=255) ttATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTACGTATCt > 2:572193/1‑76 (MQ=255) ttATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCt < 1:1432634/76‑1 (MQ=255) tATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCtg < 2:294674/76‑1 (MQ=255) aTGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCtgt < 1:1107122/76‑1 (MQ=255) tGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCtgtt < 1:1297039/76‑1 (MQ=255) tGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCtgtt < 2:780011/76‑1 (MQ=255) aGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCtgttgt > 1:482302/1‑76 (MQ=255) aGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCtgttgt > 2:993774/1‑76 (MQ=255) gTTCCGATGCCAGAGCGGCAGCGGGTTTATCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTtgtg < 2:1261937/76‑1 (MQ=255) gTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTtgtg < 2:275056/76‑1 (MQ=255) gTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTtgtg < 2:1538529/76‑1 (MQ=255) gTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTtgtg < 2:1261926/76‑1 (MQ=255) ttCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTGATCAACGCCTCGTAAGTATCTGTtgtg < 1:10112/76‑1 (MQ=255) tCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGaa < 1:812299/76‑1 (MQ=255) tCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGaa < 1:65839/76‑1 (MQ=255) tCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGaa > 1:722502/1‑76 (MQ=255) ccGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGaaa < 1:1184320/76‑1 (MQ=255) ccGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGaaa < 1:1184326/76‑1 (MQ=255) gATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAAtt < 2:1442560/76‑1 (MQ=255) cAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGAttt < 1:496433/76‑1 (MQ=255) cAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGAttt < 2:1032629/76‑1 (MQ=255) cAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGAttt < 2:755841/76‑1 (MQ=255) agagCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGAtttt < 1:1450833/76‑1 (MQ=255) agagCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGAtttt < 2:283270/76‑1 (MQ=255) agagCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGAtttt < 2:1671282/76‑1 (MQ=255) agCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCa < 2:314630/76‑1 (MQ=255) cGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATc > 2:1072221/1‑76 (MQ=255) gggTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCtt > 1:554677/1‑76 (MQ=255) gggTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCtt < 1:509468/76‑1 (MQ=255) ggTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTa < 2:556302/76‑1 (MQ=255) gTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTAt > 1:841244/1‑76 (MQ=255) tGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCt > 1:703315/1‑76 (MQ=255) gTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCtt < 2:1094176/76‑1 (MQ=255) gTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCtt > 1:1060515/1‑76 (MQ=255) cttcttTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCg > 1:803125/1‑76 (MQ=255) cttcttTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCg < 1:337700/76‑1 (MQ=255) cttcttTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCg < 1:337693/76‑1 (MQ=255) tcttTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCGTg > 1:948742/1‑76 (MQ=255) gttgGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCGTGAGatt > 1:625819/1‑76 (MQ=255) ttgGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCGTGAGatta > 2:1555994/1‑76 (MQ=255) gCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCGTGAGAttattt < 2:1431788/76‑1 (MQ=255) gCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCGTGAGAttattt < 2:97845/76‑1 (MQ=255) gCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCGTGAGAttattt < 1:981301/76‑1 (MQ=255) | TCTTTGCTAAGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTGATCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCGTGAGATTATTT > REL606/2141254‑2141396 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |