| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,613,043 | +TT | intergenic (‑104/+79) | dctA ← / ← yhjK | C4‑dicarboxylate transport protein/predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,613,041 | 1 | . | T | 92.1% | 140.1 / 5.6 | 38 | intergenic (‑102/+81) | dctA/yhjK | C4‑dicarboxylate transport protein/predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base T (17/18); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
| * | REL606 | 3,613,041 | 2 | . | T | 92.1% | 138.5 / 5.6 | 38 | intergenic (‑102/+81) | dctA/yhjK | C4‑dicarboxylate transport protein/predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base T (17/18); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
CTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAA‑‑TTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCA > REL606/3612967‑3613110 || cTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAA‑‑t > 1:1335265/1‑76 (MQ=255) ttGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAA‑‑tt < 1:1795503/76‑1 (MQ=255) ttGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAA‑‑tt > 2:1107717/1‑76 (MQ=255) ttGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAA‑‑tt < 1:601271/76‑1 (MQ=255) tGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAA‑‑ttt > 1:1465390/1‑75 (MQ=255) tGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAA‑‑ttt > 1:773383/1‑75 (MQ=255) gTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAA‑‑TTGa > 1:154617/1‑76 (MQ=255) tAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAA‑‑ttttg < 2:441422/76‑4 (MQ=255) tGGGGTTCCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACaaaa < 2:1287826/76‑1 (MQ=255) gggTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAg < 1:1625983/76‑1 (MQ=255) tgTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAgg > 2:98498/2‑76 (MQ=255) tACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGtt < 2:1034191/76‑1 (MQ=255) ccTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTaa > 1:693744/1‑76 (MQ=255) gCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTaaaaa > 2:790014/1‑76 (MQ=255) cGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAg > 1:1494617/1‑76 (MQ=255) gCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAA‑‑TTGAAACAAAAAGGTTAAAAAGTTTg < 2:1795764/76‑1 (MQ=255) ccTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGttt < 1:624849/76‑1 (MQ=255) cTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTg < 2:367543/76‑1 (MQ=255) cTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTg < 2:1090287/76‑1 (MQ=255) tAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAg > 1:1322193/1‑76 (MQ=255) tAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAg > 1:1415020/1‑76 (MQ=255) tAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGc > 2:565352/1‑76 (MQ=255) aaCACCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCt > 1:1282330/1‑76 (MQ=255) acacCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTc > 2:971663/1‑76 (MQ=255) acCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGc < 1:1281266/76‑1 (MQ=255) acCCAGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGc < 1:80642/76‑1 (MQ=255) aGACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACaa < 1:1140780/76‑1 (MQ=255) gACAACATCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACaaa < 1:685332/76‑1 (MQ=255) acaacaTCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAc < 1:369010/76‑1 (MQ=255) acaacaTCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAc < 1:1247166/76‑1 (MQ=255) caacaTCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAcc < 2:1297575/76‑1 (MQ=255) acaTCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAg < 2:780000/76‑1 (MQ=255) tCACAGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCAc < 1:183137/76‑1 (MQ=255) acaGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACtt > 1:1540123/1‑76 (MQ=255) caGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttt > 2:1492691/1‑76 (MQ=255) caGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttt < 2:60927/76‑1 (MQ=255) aGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACtttt > 2:1174780/1‑76 (MQ=255) aGAAATGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACtttt < 2:1042136/76‑1 (MQ=255) aaaTGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTa > 1:252053/1‑76 (MQ=255) aaTGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCCCTTTTTaa < 1:116341/76‑1 (MQ=255) tGTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAg > 1:1089943/1‑76 (MQ=255) gTACCTGGATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGt > 1:950712/1‑76 (MQ=255) ggATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTa > 1:1431641/1‑76 (MQ=255) ggATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTa < 1:565352/76‑1 (MQ=255) ggATCATAATTTTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTa > 1:1172569/1‑76 (MQ=255) aTCATAA‑‑TTGAAACAAAAAGGTCAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCa > 1:1432261/1‑76 (MQ=255) || CTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAA‑‑TTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCA > REL606/3612967‑3613110 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |