Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,268,960	T→G	L132R (CTG→CGG)	atoC →	fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,268,960	0	T	G	100.0%	147.8 / NA	36	L132R (CTG→CGG)	atoC	fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/18);  total (18/18)

GATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACATCTG  >  REL606/2268887‑2269032                                                                          |                                                                         gATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGc                                                                        <  1:1109740/76‑1 (MQ=255) gATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGc                                                                        >  1:502154/1‑76 (MQ=255)  aTCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCa                                                                       <  1:41028/76‑1 (MQ=255)  aTCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCa                                                                       <  1:33330/76‑1 (MQ=255)    ctcGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCAcc                                                                     <  1:1237723/76‑1 (MQ=255)     tcGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCa                                                                    <  2:1505940/76‑1 (MQ=255)         tGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCa                                                                <  2:884889/76‑1 (MQ=255)         tGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCa                                                                <  2:367041/76‑1 (MQ=255)         tGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCa                                                                >  1:248680/1‑76 (MQ=255)             ttGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGa                                                            >  2:796784/1‑76 (MQ=255)             ttGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGa                                                            <  1:1704114/76‑1 (MQ=255)              tGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAg                                                           <  1:323908/76‑1 (MQ=255)               gAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGc                                                          >  1:25771/1‑76 (MQ=255)                   ttAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCa                                                      >  1:330878/1‑76 (MQ=255)                       tCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTg                                                  <  1:1354982/76‑1 (MQ=255)                        cGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTgg                                                 >  1:613679/1‑76 (MQ=255)                          ttCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCa                                               <  2:927080/76‑1 (MQ=255)                             aGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTAGCAATg                                            >  2:1471556/1‑76 (MQ=255)                                  gcTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGc                                       <  1:1544627/76‑1 (MQ=255)                                  gcTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGc                                       <  1:1281416/76‑1 (MQ=255)                                  gcTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGc                                       >  1:153329/1‑76 (MQ=255)                                  gcTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGc                                       >  1:970668/1‑76 (MQ=255)                                         aaCTCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATtct                                <  2:636941/76‑1 (MQ=255)                                            tCCAGTCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCAc                             <  1:780752/76‑1 (MQ=255)                                                 tCAATGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACa                        <  1:838684/76‑1 (MQ=255)                                                   aaTGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGc                      <  1:129844/76‑1 (MQ=255)                                                   aaTGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGc                      <  1:250914/76‑1 (MQ=255)                                                    aTGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGcc                     <  1:1653838/76‑1 (MQ=255)                                                     tGAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGccc                    >  2:175147/1‑76 (MQ=255)                                                      gAAAAAAGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCg                   >  1:1744501/1‑76 (MQ=255)                                                         aaaaGAGATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCg                >  2:655006/1‑76 (MQ=255)                                                            agagATCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCgatg             <  2:365367/76‑1 (MQ=255)                                                                 tCCGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGa        >  1:1287214/1‑76 (MQ=255)                                                                  ccGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGAc       >  2:750324/1‑76 (MQ=255)                                                                   cGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGTGGCACATTCTCACCAACAGCCCGGCGATGATGGACa      >  2:1257972/1‑76 (MQ=255)                                                                   cGTCATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACa      >  1:684121/1‑76 (MQ=255)                                                                      cATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACATCt   >  2:980911/1‑76 (MQ=255)                                                                      cATCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACATCt   >  1:1421782/1‑76 (MQ=255)                                                                       aTCGGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACATCTg  >  1:378855/1‑76 (MQ=255)                                                                          |                                                                         GATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACATCTG  >  REL606/2268887‑2269032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑