| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,145,769 | C→T | intergenic (+100/‑95) | dnaG → / → rpoD | DNA primase/RNA polymerase sigma factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,145,769 | 0 | C | T | 96.7% | 223.8 / ‑0.5 | 61 | intergenic (+100/‑95) | dnaG/rpoD | DNA primase/RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (24/35); total (24/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01 | |||||||||||
ATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATG > REL606/3145696‑3145841 | aTCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTtggtgg > 2:807213/1‑76 (MQ=255) tCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTggtggt < 2:1229439/76‑1 (MQ=255) cGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTa < 1:702554/76‑1 (MQ=255) gggAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTaa < 1:1438909/76‑1 (MQ=255) gggAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTaa < 1:116099/76‑1 (MQ=255) gggAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTaa < 1:1103/76‑1 (MQ=255) gAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAaac < 1:1659343/76‑1 (MQ=255) aaGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAaaca < 2:583530/76‑1 (MQ=255) aaGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAaaca > 2:1188067/1‑76 (MQ=255) aaGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAaaca < 2:1518497/76‑1 (MQ=255) aGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAaacaa < 1:934514/76‑1 (MQ=255) gCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAaacaac > 2:1741113/1‑76 (MQ=255) cccGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGt > 1:1122453/1‑76 (MQ=255) cGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTg < 1:1236960/76‑1 (MQ=255) cGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTg > 1:35694/1‑76 (MQ=255) cAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGAt > 2:859844/1‑76 (MQ=255) cAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGAt > 2:527283/1‑76 (MQ=255) cAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGAt > 2:420154/1‑76 (MQ=255) aGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGAtt < 1:474285/76‑1 (MQ=255) gCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGAttt < 2:100033/76‑1 (MQ=255) gCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGAttt < 2:1667216/76‑1 (MQ=255) gCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGAttt < 1:1736781/76‑1 (MQ=255) gCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGAttt < 1:967762/76‑1 (MQ=255) ccGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTc < 2:452894/76‑1 (MQ=255) cGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCa < 2:1031382/76‑1 (MQ=255) cGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCa < 2:1615341/76‑1 (MQ=255) cGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCa < 1:613316/76‑1 (MQ=255) gCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAg < 1:626987/76‑1 (MQ=255) cTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGt < 1:386243/76‑1 (MQ=255) gagaGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTa < 1:1081816/76‑1 (MQ=255) tgagGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTaa > 2:1453723/2‑76 (MQ=255) gagGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAAc < 2:799498/76‑1 (MQ=255) cAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCt > 1:1093107/1‑76 (MQ=255) cAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCt > 1:1243042/1‑76 (MQ=255) aGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGcca > 1:889763/1‑74 (MQ=255) aGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTg > 1:573483/1‑76 (MQ=255) aGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTg > 1:1415031/1‑76 (MQ=255) aGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTg < 1:93729/76‑1 (MQ=255) aaaTATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGAc < 1:848991/76‑1 (MQ=255) aaaTATATAAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGAc > 1:3087/1‑76 (MQ=255) aaTATATAAGTACGCCCTCGTACTTATCGTTGGTGGTAAACAACCTTTGGATTTCAGCGTTAACGGCTAAAAGaaa > 2:1481550/1‑74 (MQ=255) tatataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATc < 2:209195/76‑1 (MQ=255) tatataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATc < 1:648096/76‑1 (MQ=255) tatataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATc < 2:1507039/76‑1 (MQ=255) tatataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATc < 1:950381/76‑1 (MQ=255) tatataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATc < 2:946337/76‑1 (MQ=255) atataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCg > 1:873074/1‑76 (MQ=255) tataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCgg < 1:449133/76‑1 (MQ=255) tataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCgg < 2:776904/76‑1 (MQ=255) ataAGTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCggg > 2:1661384/1‑76 (MQ=255) aGTACGCCCTCGTAATTATCGTTGGTGTTAAACAACCGTTGGATTTCAGCGTTAACGGCTGACGGACATCGGGTCa < 2:1024206/76‑1 (MQ=255) gTACGCCCTCGTAATTATCGTTGGTGGTAAACAACCGTTGGGTTTCAGCGTTAACGGCTGCAGGACATCGAGGCaa > 2:712215/1‑76 (MQ=255) cGTAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAac > 2:1385306/1‑76 (MQ=255) gTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAaca < 1:1622594/76‑1 (MQ=255) tAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAacac > 2:344145/1‑76 (MQ=255) tAATTATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAacac > 2:956806/1‑76 (MQ=255) ttATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCaa < 2:1219763/76‑1 (MQ=255) tATCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAAc > 1:1197762/1‑76 (MQ=255) aTCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAAcc > 2:192868/1‑76 (MQ=255) aTCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAAcc > 1:983705/1‑76 (MQ=255) tCGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCt < 1:1115955/76‑1 (MQ=255) cGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTc < 1:96137/76‑1 (MQ=255) cGTTGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTc < 2:843380/76‑1 (MQ=255) ttGGTGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCAt > 2:1125398/1‑76 (MQ=255) tggtggTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATg < 2:212490/76‑1 (MQ=255) tggtggTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATg < 2:1665869/76‑1 (MQ=255) | ATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATG > REL606/3145696‑3145841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |