| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,101,777 | A→C | intergenic (‑7/+86) | hslV ← / ← ftsN | ATP‑dependent protease peptidase subunit/essential cell division protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,101,777 | 0 | A | C | 96.2% | 203.5 / ‑1.6 | 53 | intergenic (‑7/+86) | hslV/ftsN | ATP‑dependent protease peptidase subunit/essential cell division protein |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (21/30); total (22/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
ACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGACCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATTATAGATGGGG > REL606/4101704‑4101847 | aCGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGccc > 1:1310091/1‑76 (MQ=255) ggCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCtt < 2:1441077/76‑1 (MQ=255) gCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTg < 2:817426/76‑1 (MQ=255) gCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTg < 1:1416273/76‑1 (MQ=255) gCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTg < 1:389488/76‑1 (MQ=255) tgcccATCGCCAGCGATTGCCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGtt < 2:166827/73‑1 (MQ=255) gACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTa > 2:993561/1‑76 (MQ=255) gACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTa > 2:440300/1‑76 (MQ=255) ccATCGCCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACg < 2:601333/76‑1 (MQ=255) aTGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTAc < 1:1136503/76‑1 (MQ=255) aTGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTAc > 1:541191/1‑76 (MQ=255) gACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACaa < 1:1525199/76‑1 (MQ=255) aCCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACaaa < 2:571400/76‑1 (MQ=255) cacaTGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAAcc < 1:942170/76‑1 (MQ=255) caTGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACCGAGTACAAAcccc > 2:437100/1‑76 (MQ=255) tGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGt < 1:276209/76‑1 (MQ=255) ggCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTa > 2:1459320/1‑76 (MQ=255) gCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTAc < 1:963175/76‑1 (MQ=255) gCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTAc < 1:868447/76‑1 (MQ=255) ccGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACa > 1:357316/1‑76 (MQ=255) aCGGCGGGCGCTTACTATAGTTGTCACGAGCTGCCCCCGTGGTTACGAATACAGAGTACAAACCACGTACAAAAGt < 1:101247/76‑1 (MQ=255) cGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTa < 2:1278548/76‑1 (MQ=255) cGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTa > 2:1643358/1‑76 (MQ=255) gTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACggg < 2:338913/76‑1 (MQ=255) tACGCTTACTATAGTTGTCACGAGCTGACCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACgggg > 1:1228049/1‑76 (MQ=255) tACCCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACgggg < 2:807371/76‑1 (MQ=255) cGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCa > 1:1247968/1‑76 (MQ=255) gCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCAt < 2:1580858/76‑1 (MQ=255) ttACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATaa > 1:1697171/1‑76 (MQ=255) ttACTATAGTTGTCACGAGCTGACCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATaa < 1:1563469/76‑1 (MQ=255) tACTGTAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTGCAAAAGTACGGGGCATAAt < 2:668883/76‑1 (MQ=255) tACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAAt > 1:1360460/1‑76 (MQ=255) tACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAAt < 1:453823/76‑1 (MQ=255) tACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGAACGGGGCATAAt > 1:255639/1‑76 (MQ=255) tACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGCACAAAAATACGGGGCATAAt > 1:1595953/1‑76 (MQ=255) tACCATAGTTGTCACGCGCTGCCCCCTTGGTTACGAATACAGAGTACAACCCCCGTACAAAAGTACGGGGCATAAt < 2:581408/76‑1 (MQ=255) aCTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATg > 1:552449/1‑76 (MQ=255) aCTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATg < 1:1075491/76‑1 (MQ=255) aCTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATg < 1:1222836/76‑1 (MQ=255) aCTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATg < 1:194602/76‑1 (MQ=255) aCTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATg < 2:347917/76‑1 (MQ=255) aCTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATg > 1:754962/1‑76 (MQ=255) taGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAAt > 1:441361/1‑76 (MQ=255) aGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAAtt < 2:1013100/76‑1 (MQ=255) aGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAAtt > 1:879838/1‑76 (MQ=255) aGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAAtt > 1:947938/1‑76 (MQ=255) aGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAAtt > 2:1550987/1‑76 (MQ=255) aGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAAAACGGGGCATAAGGCAAtt > 1:687333/1‑76 (MQ=255) gTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATta > 1:1381464/1‑76 (MQ=255) gTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATta < 2:848111/76‑1 (MQ=255) ttGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATtat > 2:233525/1‑76 (MQ=255) ttGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATtat < 2:520884/76‑1 (MQ=255) ttGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATtat < 2:554729/76‑1 (MQ=255) gTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATTATAg < 2:1009737/76‑1 (MQ=255) aggAGCTGCCCCCCTCGTTACGAAAACAGAGAACAAGCGCAGTAGAAAAGGCAAGAGCATAATGCAATTATATATg < 2:498227/74‑1 (MQ=255) cGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATTATAGATgg > 2:1655113/1‑76 (MQ=255) aGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATTATAGATgggg > 1:290327/1‑76 (MQ=255) | ACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGACCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATTATAGATGGGG > REL606/4101704‑4101847 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |