| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,004,196 | T→A | intergenic (‑122/+480) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,004,196 | 0 | T | A | 98.7% | 310.6 / ‑5.4 | 77 | intergenic (‑122/+480) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (41/35); total (42/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAATAT > REL606/1004124‑1004268 | tAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCa > 1:1711481/1‑76 (MQ=255) aaGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCAt > 2:925847/1‑76 (MQ=255) aGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCAtt > 1:1663654/1‑76 (MQ=255) aGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCAtt > 1:669934/1‑76 (MQ=255) aGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCAtt < 2:111479/76‑1 (MQ=255) aGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCAtt > 2:739926/1‑76 (MQ=255) cTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTaaaa < 1:1078877/76‑1 (MQ=255) cTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTaaaa > 2:1450633/1‑76 (MQ=255) cTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTaaaa > 2:1450614/1‑76 (MQ=255) gTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACt > 1:1081063/1‑76 (MQ=255) cAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTaa < 2:1689688/76‑1 (MQ=255) cAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTaa > 1:1755978/1‑76 (MQ=255) aaTAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTagc < 1:447448/76‑3 (MQ=255) aaTAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAAc < 1:447430/76‑1 (MQ=255) aaaTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTc < 1:401285/76‑1 (MQ=255) aaaTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTc < 1:740859/76‑1 (MQ=255) aaTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCg < 2:1732267/76‑1 (MQ=255) tttACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCt > 1:1668434/1‑76 (MQ=255) tttACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCt < 1:986801/76‑1 (MQ=255) tttACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCt > 1:18743/1‑76 (MQ=255) ttACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTa < 1:830398/76‑1 (MQ=255) tACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTAc > 2:1165292/1‑76 (MQ=255) cGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCg > 2:543533/1‑76 (MQ=255) ggAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGt > 1:1765664/1‑76 (MQ=255) ggAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGt < 2:217065/76‑1 (MQ=255) gAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTg > 1:517968/1‑76 (MQ=255) gAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTg > 2:382244/1‑76 (MQ=255) aaCTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGa > 2:798688/1‑76 (MQ=255) ttGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTg < 1:1065100/76‑1 (MQ=255) ttGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTg < 1:455073/76‑1 (MQ=255) gatgaGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCt > 1:831916/1‑76 (MQ=255) atgaGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGAGCTa > 2:1200629/1‑76 (MQ=255) tgaGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTAc < 2:1074249/76‑1 (MQ=255) gagaGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACa > 2:1511943/1‑76 (MQ=255) gagaGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACa < 1:303429/76‑1 (MQ=255) gagaGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACa < 2:1551073/76‑1 (MQ=255) gagaGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACa > 2:574367/1‑76 (MQ=255) agagTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaa > 2:970164/1‑76 (MQ=255) agagTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaa < 2:550325/76‑1 (MQ=255) agTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaag < 1:1366757/76‑1 (MQ=255) tttGGAGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagat > 1:409857/1‑76 (MQ=255) ttGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagata < 1:1319910/76‑1 (MQ=255) ttGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagata < 1:457046/76‑1 (MQ=255) tGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataa < 1:570468/76‑1 (MQ=255) tGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataa < 1:275463/76‑1 (MQ=255) tGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataa < 2:1146481/76‑1 (MQ=255) ggTGTCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaa < 2:1252162/76‑1 (MQ=255) tgtCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaaga < 1:34995/76‑1 (MQ=255) tgtCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaaga > 1:1522726/1‑76 (MQ=255) gtCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaagat > 1:1151765/1‑76 (MQ=255) gtCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaagat < 1:352094/76‑1 (MQ=255) gtCTTTATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaagat > 1:784290/1‑76 (MQ=255) ttATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTgg < 2:844843/76‑1 (MQ=255) ttATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTgg > 2:1729660/1‑76 (MQ=255) tATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGt > 1:1296288/1‑76 (MQ=255) tATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGt > 2:268553/1‑76 (MQ=255) tATGTGTCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGt > 2:268535/1‑76 (MQ=255) gtgtCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTc < 2:954993/76‑1 (MQ=255) gtgtCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTc < 2:954988/76‑1 (MQ=255) tgtCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCa > 1:1565871/1‑76 (MQ=255) tCTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaa > 2:195187/1‑76 (MQ=255) cTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaa > 1:783323/1‑76 (MQ=255) cTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaa < 1:282636/76‑1 (MQ=255) cTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaa < 1:1367110/76‑1 (MQ=255) cTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaa > 1:1027268/1‑76 (MQ=255) cTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaa < 1:1626651/76‑1 (MQ=255) cTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaa > 1:1217847/1‑76 (MQ=255) cTGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaa < 1:282650/76‑1 (MQ=255) tGCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaaa < 1:1775552/76‑1 (MQ=255) gCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAg > 2:1324531/1‑76 (MQ=255) gCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAg > 2:520827/1‑76 (MQ=255) gCAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAg > 2:1745091/1‑76 (MQ=255) cAGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGa > 1:329927/1‑76 (MQ=255) nggCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaa > 1:1489560/2‑76 (MQ=255) aGGCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaa > 1:1304820/1‑76 (MQ=255) gCATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaaa < 1:615992/76‑1 (MQ=255) cATCTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaaaa > 2:324329/1‑76 (MQ=255) cTTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAatat < 1:535650/76‑1 (MQ=255) | TAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAATAT > REL606/1004124‑1004268 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |