| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,949,211 | G→A | Q182* (CAG→TAG) | tap ← | methyl‑accepting protein IV |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,949,211 | 0 | G | A | 97.9% | 179.3 / ‑3.6 | 48 | Q182* (CAG→TAG) | tap | methyl‑accepting protein IV |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (21/26); total (21/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01 | |||||||||||
CACTGCTGATGTAGATCGCCGCAACAATAATCATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTGGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCAAAGTTAACGTCAAACG > REL606/1949139‑1949282 | cACTGCTGATGTAGATCGCCGCAACAATAATCATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCt < 1:896172/76‑1 (MQ=255) aCTGCTGATGTAGATCGCCGCAACAATAATCATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCta < 2:705596/76‑2 (MQ=255) gctgATGTAGATCGCCGCAACAATAATCATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTgc < 2:1500217/76‑1 (MQ=255) gctgATGTAGATCGCCGCAACAATAATCATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTgc > 2:635162/1‑76 (MQ=255) aTCGCCGCAACAATAATCATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTggcgg > 2:556862/1‑76 (MQ=255) cgccgcAACAATAATCATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCt < 1:763725/76‑1 (MQ=255) ccgcAACAATAATCATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTc > 1:1197253/1‑76 (MQ=255) ccgcAACAATAATCATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTc < 1:160959/76‑1 (MQ=255) gcAACAATAATCATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCa > 2:1241919/1‑76 (MQ=255) caacaaTAATCATGCTGATAAACACCAGTGCCTAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAg > 1:912113/1‑76 (MQ=255) aataatCATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGcaca < 2:1061502/76‑1 (MQ=255) aataatCATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGcaca < 1:339664/76‑1 (MQ=255) ataatCATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACAt > 1:1510206/1‑76 (MQ=255) aatCATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATgg > 2:1402324/1‑76 (MQ=255) tCATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGtt > 1:969230/1‑76 (MQ=255) cATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTg < 2:308481/76‑1 (MQ=255) cTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGAtct > 1:1007247/1‑76 (MQ=255) tGATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGAtctc > 2:1610587/1‑76 (MQ=255) gATAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTcc > 2:644234/1‑76 (MQ=255) aTAAACACCAGTGCCGAAATCTGATAGTTACGCTGGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCa < 2:1275062/76‑1 (MQ=255) aTAAACACCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCa > 1:1076353/1‑76 (MQ=255) acacCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTg > 1:1681041/1‑76 (MQ=255) cacCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCGCCTGGGTGGTCTCCAGCTGc > 2:75350/1‑76 (MQ=255) acCAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGcc < 2:562319/76‑1 (MQ=255) ccAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCCCTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCa < 1:1050137/76‑1 (MQ=255) ccAGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCa < 2:1626997/76‑1 (MQ=255) ccAGTGCCGAAATCTGATAGTTACACTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCa < 1:407207/76‑1 (MQ=255) aGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAgg < 1:1266131/76‑1 (MQ=255) aGTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAgg < 1:1698607/76‑1 (MQ=255) gTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGc < 1:123573/76‑1 (MQ=255) gTGCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGc < 1:1594420/76‑1 (MQ=255) gCCGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCt < 2:1056748/76‑1 (MQ=255) cGAAATCTGATAGTTACGCTAGCTTTGCGCCCTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCa < 2:939543/76‑1 (MQ=255) cGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCa < 2:1056393/76‑1 (MQ=255) cGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCa < 2:939559/76‑1 (MQ=255) cGAAATCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCa < 1:7861/76‑1 (MQ=255) aTCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCCGGCCTCAAAGt > 1:1356779/1‑76 (MQ=255) aTCTGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCAAAGt > 1:1025292/1‑76 (MQ=255) tGATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCAAAGTTaa > 2:1195042/1‑76 (MQ=255) gATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCAAAGTTAAc < 1:792590/76‑1 (MQ=255) gATAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCAAAGTTAAc > 1:741125/1‑76 (MQ=255) aTAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCAAAGTTAACg > 1:1531331/1‑76 (MQ=255) aTAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCAAAGTTAACg < 1:1464537/76‑1 (MQ=255) aTAGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCAAAGATAACg > 1:310792/1‑76 (MQ=255) aGTTACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCAAAGTTAACGTc < 1:1265844/76‑1 (MQ=255) ttACGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCAAAGTTAACGTCaa > 2:29046/1‑76 (MQ=255) aCGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCAAAGTTAACGTCAAAc < 1:1095744/76‑1 (MQ=255) cGCTAGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCAAAGTTAACGTCAAACg < 1:1424274/76‑1 (MQ=255) | CACTGCTGATGTAGATCGCCGCAACAATAATCATGCTGATAAACACCAGTGCCGAAATCTGATAGTTACGCTGGCTTTGCGCACTGGCGGCTTCCAGCACATGGTTGATCTCCAGCTGCCAGGCCTCAAAGTTAACGTCAAACG > REL606/1949139‑1949282 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |