| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 475,241 | C→A | G22V (GGC→GTC) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 475,241 | 0 | C | A | 98.0% | 186.5 / NA | 50 | G22V (GGC→GTC) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (23/26); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTC > REL606/475168‑475313 | gAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACg < 1:617647/76‑1 (MQ=255) aaTGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGa < 2:1318285/76‑1 (MQ=255) tGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAgg < 1:1243891/76‑1 (MQ=255) ggTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGa < 1:1457032/76‑1 (MQ=255) gTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGAt < 1:325499/76‑1 (MQ=255) gTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGAt < 1:1510195/76‑1 (MQ=255) tCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATa < 1:654422/76‑1 (MQ=255) ccTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATaa < 1:383315/76‑1 (MQ=255) cAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCg > 2:847295/1‑76 (MQ=255) aGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGa < 1:426553/76‑1 (MQ=255) cacaCCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGc < 1:1365357/76‑1 (MQ=255) acacCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGca > 2:791169/1‑76 (MQ=255) cccGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACaa < 1:839512/76‑1 (MQ=255) ccGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAg > 1:1424814/1‑76 (MQ=255) ccGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAg < 2:121943/76‑1 (MQ=255) ccGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAg < 1:983413/76‑1 (MQ=255) aaCAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGgccgc > 1:233186/1‑76 (MQ=255) aCAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGgccgcc > 2:1368988/1‑76 (MQ=255) tatCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCcaacaa < 1:575117/76‑1 (MQ=255) atCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAAt < 1:211426/76‑1 (MQ=255) cccACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAAtgg > 1:301417/1‑76 (MQ=255) caccaGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAAtggtg < 1:1388213/76‑1 (MQ=255) caccaGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAAtggtg > 1:443233/1‑76 (MQ=255) caccaGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAAtggtg > 1:1006274/1‑76 (MQ=255) caGAGGAGAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGgtggtg > 2:924726/1‑76 (MQ=255) aggagAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATa > 2:520324/1‑76 (MQ=255) ggagAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATaa > 1:513409/1‑76 (MQ=255) ggagAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATaa < 1:952702/76‑1 (MQ=255) ggagAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATaa < 2:615851/76‑1 (MQ=255) ggagAAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATaa < 2:1534479/76‑1 (MQ=255) gagaAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAg > 2:1070369/1‑76 (MQ=255) gagaAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAg < 2:230378/76‑1 (MQ=255) cgaAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGc > 2:619059/2‑76 (MQ=255) agaAATACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGc > 2:1157241/1‑76 (MQ=255) aTACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCgggg < 1:1113345/76‑1 (MQ=255) aTACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCgggg < 1:1113354/76‑1 (MQ=255) tACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCggggg > 1:76657/1‑75 (MQ=255) tACGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGt < 1:1161675/76‑1 (MQ=255) aCGTAGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTg < 1:260383/76‑1 (MQ=255) agcttATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGt > 2:1172858/4‑76 (MQ=255) aGTTTATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGt > 1:5792/1‑76 (MQ=255) ttATTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGAt > 1:167340/1‑76 (MQ=255) aTTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGc < 1:1212963/76‑1 (MQ=255) aTTGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGc < 1:1503025/76‑1 (MQ=255) ttGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCa < 1:151721/76‑1 (MQ=255) tGGCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCAt > 1:512574/1‑76 (MQ=255) ggCCAGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCAtt > 1:1331327/1‑76 (MQ=255) aGCATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGAGGGGTGATGCATGCCGt > 2:1455726/1‑76 (MQ=255) cATGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGtct > 2:1345512/1‑76 (MQ=255) aTGACGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGtctc > 1:642758/1‑76 (MQ=255) | GAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTC > REL606/475168‑475313 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |