| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,169,030 | T→C | I1357T (ATC→ACC) | rpoC → | DNA‑directed RNA polymerase subunit beta' |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,169,030 | 0 | T | C | 98.0% | 194.5 / ‑4.5 | 49 | I1357T (ATC→ACC) | rpoC | DNA‑directed RNA polymerase subunit beta' |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (26/22); total (27/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
AAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGC > REL606/4168961‑4169102 | aaGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGc > 2:929859/1‑76 (MQ=255) gcagcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAg < 2:368627/76‑1 (MQ=255) ccgcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAgg < 2:420665/74‑1 (MQ=255) cagcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAgg < 1:652763/76‑1 (MQ=255) cGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTAcc > 2:1499115/1‑76 (MQ=255) tGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGt < 2:952959/76‑1 (MQ=255) gCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGtt < 2:1042822/76‑1 (MQ=255) gggCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTAc < 1:233019/76‑1 (MQ=255) ggCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTAcg < 2:1040108/76‑1 (MQ=255) gCAGACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTATCGGCTAcgc > 2:110787/1‑76 (MQ=255) gCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTAcgc < 2:824692/76‑1 (MQ=255) aaaCGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGt > 2:768325/1‑76 (MQ=255) cgaACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAg < 2:668949/76‑1 (MQ=255) gaACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAgg < 2:843767/76‑1 (MQ=255) aaCTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGa < 1:1413441/76‑1 (MQ=255) aCTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGAt < 2:1226305/76‑1 (MQ=255) aCTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGAt < 2:967027/76‑1 (MQ=255) tGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCg < 2:7761/76‑1 (MQ=255) gcgcGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATGGt > 2:23874/1‑76 (MQ=255) gcgcGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGt < 1:456104/76‑1 (MQ=255) cgGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATg < 2:28313/76‑1 (MQ=255) ggCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGc < 1:682533/76‑1 (MQ=255) gCCTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCg > 1:142234/1‑76 (MQ=255) cTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTc > 2:1079433/1‑76 (MQ=255) cTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTc > 2:130740/1‑76 (MQ=255) cTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTc < 2:1324366/76‑1 (MQ=255) cTGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTc < 1:625322/76‑1 (MQ=255) tGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCg > 1:920093/1‑76 (MQ=255) tGAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCg < 1:308846/76‑1 (MQ=255) gAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGc > 1:77293/1‑76 (MQ=255) gAAAGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGc > 2:59889/1‑76 (MQ=255) aaaGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGcc > 1:1059978/1‑76 (MQ=255) aaaGAGAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGcc > 2:695491/1‑76 (MQ=255) aaaGAGAACGTTATCGTGGGGCGTCTGATCCCGGCAGCTATAGGGTACGCGCACCACCTGGATTTTTTGCGTCGcc > 2:540503/1‑76 (MQ=255) aaaGAGAACGTTATCGTGGCTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGcc < 1:643928/76‑1 (MQ=255) agagAACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGt > 2:696524/1‑76 (MQ=255) gagaACGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGtg > 2:1385993/1‑76 (MQ=255) aCGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGtgctgc > 1:740738/1‑76 (MQ=255) cGTTATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCg > 1:1503119/1‑76 (MQ=255) ttATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCggg > 2:1498026/1‑76 (MQ=255) ttATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCggg > 1:690866/1‑76 (MQ=255) ttATCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGCCGCCGGGGCGCggg > 1:675094/1‑76 (MQ=255) aTCGTGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCggggg > 1:1511425/1‑74 (MQ=255) tGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGc > 2:201323/1‑76 (MQ=255) tGGGTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGGGAAGc > 2:1450204/1‑76 (MQ=255) gggTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCt > 1:721405/1‑76 (MQ=255) ggTCGTCTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTc > 2:1519665/1‑76 (MQ=255) gtcTGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGc < 1:170142/76‑1 (MQ=255) tGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGc > 2:1026736/1‑76 (MQ=255) tGACCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGc > 2:1360722/1‑76 (MQ=255) | AAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGC > REL606/4168961‑4169102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |