Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,285,884	G→A	intergenic (‑190/+40)	yhbL ← / ← arcB	isoprenoid biosynthesis protein with amidotransferase‑like domain/hybrid sensory histidine kinase in two‑component regulatory system with ArcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,285,884	0	G	A	100.0%	237.7 / NA	59	intergenic (‑190/+40)	yhbL/arcB	isoprenoid biosynthesis protein with amidotransferase‑like domain/hybrid sensory histidine kinase in two‑component regulatory system with ArcA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/29);  total (30/29)

CGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAG  >  REL606/3285811‑3285959                                                                          |                                                                            cgtgcgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAgt                                                                           >  1:1276075/1‑76 (MQ=255)  gtgcgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAgtg                                                                          >  1:603810/1‑76 (MQ=255)          tGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCa                                                                  >  1:742733/1‑76 (MQ=255)            aaaaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGt                                                                >  1:647958/1‑76 (MQ=255)             aaaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTa                                                               <  2:967968/76‑1 (MQ=255)             aaaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTa                                                               >  2:1552615/1‑76 (MQ=255)              aaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTAt                                                              <  1:1231251/76‑1 (MQ=255)                aCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTc                                                            >  2:1567006/1‑76 (MQ=255)                aCAGTGCTGTGCCCTTGTAACCCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTc                                                            >  2:1037382/1‑76 (MQ=255)                 cAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTcg                                                           <  2:218993/76‑1 (MQ=255)                      cTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCAc                                                      >  2:1151150/1‑76 (MQ=255)                       tgtgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCAcc                                                     <  2:1474721/76‑1 (MQ=255)                        gtgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCAccc                                                    >  1:494295/1‑76 (MQ=255)                        gtgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCAccc                                                    <  1:1391748/76‑1 (MQ=255)                         tgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCAcccc                                                   >  2:337282/1‑76 (MQ=255)                         tgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCAcccc                                                   >  2:443245/1‑76 (MQ=255)                         tgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCAcccc                                                   >  2:69689/1‑76 (MQ=255)                         tgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCAcccc                                                   <  1:935850/76‑1 (MQ=255)                          gCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCg                                                  <  1:177135/76‑1 (MQ=255)                          gCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCg                                                  <  2:1531663/76‑1 (MQ=255)                           cccTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCgg                                                 <  2:50992/76‑1 (MQ=255)                             cTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTc                                               <  1:658454/76‑1 (MQ=255)                               tGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTa                                             >  1:1039832/1‑76 (MQ=255)                               tGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTa                                             <  2:478134/76‑1 (MQ=255)                               tGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTa                                             <  2:722969/76‑1 (MQ=255)                               tGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACACCGGTCTa                                             >  1:220131/1‑76 (MQ=255)                                gTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAg                                            <  2:940197/76‑1 (MQ=255)                                gTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAg                                            <  1:389976/76‑1 (MQ=255)                                 tAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTAGCGCAGTATTCGCGCACCCCGGTCTAGc                                           >  1:1412636/1‑76 (MQ=255)                                  aaCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGcc                                          <  2:44476/76‑1 (MQ=255)                                   aCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCg                                         >  1:1157029/1‑76 (MQ=255)                                   aCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCg                                         <  2:1404510/76‑1 (MQ=255)                                     tcatcaTAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCggg                                       <  2:447547/76‑1 (MQ=255)                                      catcatAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCgggg                                      >  1:523301/1‑76 (MQ=255)                                       atcatAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGt                                     >  1:859010/1‑76 (MQ=255)                                       atcatAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGt                                     <  2:1393306/76‑1 (MQ=255)                                       atcatAATAATTGACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGt                                     <  2:1408901/76‑1 (MQ=255)                                         catAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCa                                   >  2:874593/1‑76 (MQ=255)                                         catAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCa                                   >  2:912958/1‑76 (MQ=255)                                         catAATAATTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGGCa                                   >  1:759795/1‑76 (MQ=255)                                           taataaTTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCAtt                                 <  1:1157171/76‑1 (MQ=255)                                           taataaTTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCAtt                                 <  2:684535/76‑1 (MQ=255)                                           taataaTTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCAtt                                 >  2:890804/1‑76 (MQ=255)                                            aataatTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCAttt                                >  2:1343696/1‑76 (MQ=255)                                            aataatTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCAttt                                <  2:174655/76‑1 (MQ=255)                                            aataatTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCAttt                                <  2:1258441/76‑1 (MQ=255)                                             ataatTTCCGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCAtttt                               <  1:1005059/76‑1 (MQ=255)                                              aaatTTAGGGCGCGGCAAAGATTCCCCTAGTGTTGGCGCAGTATTCGCGCCCCCCGGTCTAGCCGGGGTCAttttt                              <  2:848598/75‑1 (MQ=255)                                               aatTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCAtttttt                             <  2:733419/76‑1 (MQ=255)                                               aatTTACGGCGCAGCCAAGATTTCCCTAGTGTGGGCGCAGTATTCGCGCTCCCCGGTCTAGCCGGGGTCAtttttt                             <  2:1290322/76‑1 (MQ=255)                                                atTTACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTa                            >  2:469418/1‑76 (MQ=255)                                                 tttACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAg                           >  2:293449/1‑76 (MQ=255)                                                  ttACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGt                          >  1:1444891/1‑76 (MQ=255)                                                  ttACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGGCATTTTTTAGt                          >  1:849006/1‑76 (MQ=255)                                                   tACGGCGCAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTg                         >  2:1019016/1‑76 (MQ=255)                                                        cgcAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCttt                    <  2:1157465/76‑1 (MQ=255)                                                        cgcAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCtta                    <  2:496174/76‑2 (MQ=255)                                                         gcAGCCAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCtttt                   <  2:1190464/76‑1 (MQ=255)                                                              cAAGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccac              >  1:49140/1‑76 (MQ=255)                                                               aaGATTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGGCATTTTTTAGTGGCTTTTGccacc             >  1:1239754/1‑76 (MQ=255)                                                                  aTTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccacccac          <  2:1406295/76‑1 (MQ=255)                                                                  aTTTCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccacccac          <  2:930050/76‑1 (MQ=255)                                                                   tttCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACg         >  2:667828/1‑76 (MQ=255)                                                                   tttCCCTAGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACg         >  2:923511/1‑76 (MQ=255)                                                                          ggTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAg  <  1:509076/76‑1 (MQ=255)                                                                          |                                                                            CGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAG  >  REL606/3285811‑3285959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑