Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,202,538	T→G	K8Q (AAA→CAA)	iclR ←	DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,202,538	0	T	G	100.0%	153.1 / NA	37	K8Q (AAA→CAA)	iclR	DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (10/27);  total (10/27)

TCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAG  >  REL606/4202466‑4202610                                                                         |                                                                         tCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCGGTgcgc                                                                       >  2:82836/1‑76 (MQ=255)       cACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTGCGCggggag                                                                 >  2:623899/1‑73 (MQ=255)        aCGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATg                                                                <  2:147221/76‑1 (MQ=255)         cgcgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTGCGCGGGGATgg                                                               >  2:278192/1‑76 (MQ=255)           cgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTGCGCGGGGATgggg                                                             >  2:1364413/1‑75 (MQ=255)              tAAAGACTGAACCTGTCCATTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCg                                                          <  1:987831/76‑1 (MQ=255)               aaaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTGCGCGGGGATGGGGGCgg                                                         >  1:853978/1‑75 (MQ=255)                aaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCGTTGCGCGGGGATGGGGGGGAc                                                        >  2:237374/1‑76 (MQ=255)                    cTGAACCTGTCCAGTCGCTCGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATg                                                    <  1:934103/76‑1 (MQ=255)                     tGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGa                                                   <  1:368645/76‑1 (MQ=255)                     tGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGa                                                   <  2:894183/76‑1 (MQ=255)                      gAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGAc                                                  <  1:950294/76‑1 (MQ=255)                       acccTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACa                                                 <  1:901357/74‑1 (MQ=255)                       aaCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACa                                                 <  2:278858/76‑1 (MQ=255)                       aaCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACa                                                 <  1:313287/76‑1 (MQ=255)                         ccTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGt                                               <  2:654551/76‑1 (MQ=255)                         ccTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGt                                               <  1:479786/76‑1 (MQ=255)                         ccTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGt                                               <  2:1461634/76‑1 (MQ=255)                           tGTCCAGTCGCTGGTGCGGTGGCACCGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGtct                                             <  2:482417/76‑1 (MQ=255)                            gTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGtctc                                            <  1:115334/76‑1 (MQ=255)                            gTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCGTTGCGCGGGGATGGGTGGGGCCCTGACAGtctc                                            >  1:1468792/1‑76 (MQ=255)                            gTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCGTTGCGCGGGGATGGGGGGGGCCATGACAGgctc                                            >  2:1137234/1‑76 (MQ=255)                                aGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCttt                                        <  1:1134882/76‑1 (MQ=255)                                  tCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTCCGCGTTGCGCGGGGATGGGGGCGGCCATGACAGTCTCCttttt                                      >  1:1002480/1‑76 (MQ=255)                                  tCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCCCGGTGCGCGGGGATGGGGGCGGGCCTGACAGCCTCCctttt                                      >  1:979139/1‑76 (MQ=255)                                    gCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTGCGCGGGGATGGGGGGGGCCCTGACAGGCTCCttttttt                                    >  1:887720/1‑74 (MQ=255)                                    gCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCGTTGCGCGGGAATGGGGGCGACCATGACAGGCTCCTTTTTCt                                    >  1:1509522/1‑76 (MQ=255)                                       ggTGCGGTGTAAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGTGTGCGACCATGACAGTCTCCTTTTTCTGTa                                 <  2:807435/76‑1 (MQ=255)                                       ggTGCGGTGGCAACGGCGGGGTTTCTGCCGCGTTGCGCGGGGATGGGGGCGACCCTGACAGTCTCCTTTTTCtttt                                 >  2:1564736/1‑73 (MQ=255)                                              tGGCAACGGCGGGGTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTTTGTATCGTGGa                          >  2:405809/1‑76 (MQ=255)                                              tGGCAACGGCGGGGTTTCTGCCGCGGTGCGCGGGGATGGGTGCGGCCATGACAGTCTCCTTTTTCTGTATCGTggg                          >  2:1229690/1‑75 (MQ=255)                                                gCAACGGCGGGGTTTCTGCCGCGGTGCGCGGGGATGGGGGCGACCATGACCGTCTCCTTTTTCTTTTTCGTGGgaa                        >  2:962062/1‑76 (MQ=255)                                                 cAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAt                       <  1:185572/76‑1 (MQ=255)                                                    cggcggGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcat                    <  1:1464046/76‑1 (MQ=255)                                                    cggcggGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcat                    >  1:1654738/1‑76 (MQ=255)                                                     ggcggGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcatt                   >  1:451484/1‑76 (MQ=255)                                                     ggcggGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcatt                   >  1:1526888/1‑76 (MQ=255)                                                       cggGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcatttt                 <  1:543854/76‑1 (MQ=255)                                                       cggGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcatttt                 <  1:442807/76‑1 (MQ=255)                                                       cggGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcatttt                 >  1:1610733/1‑76 (MQ=255)                                                       cggGTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcatttt                 >  1:1593620/1‑76 (MQ=255)                                                         ggTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttca               >  2:1150329/1‑76 (MQ=255)                                                          gTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttcat              <  1:1529730/76‑1 (MQ=255)                                                          gTTTTCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttcat              <  1:1106073/76‑1 (MQ=255)                                                            tttCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttcattt            >  2:827311/1‑76 (MQ=255)                                                            tttCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttcattt            >  1:484664/1‑76 (MQ=255)                                                             ttCTGCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATcattttcatttt           <  1:340871/76‑1 (MQ=255)                                                                 gCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTAtt       <  2:903782/76‑1 (MQ=255)                                                                 gCCGCGTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTAtt       >  2:950152/1‑76 (MQ=255)                                                                    gcgTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGtt    <  1:1142886/76‑1 (MQ=255)                                                                      gTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAg  <  2:693996/76‑1 (MQ=255)                                                                      gTTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAg  <  1:108856/76‑1 (MQ=255)                                                                         |                                                                         TCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAG  >  REL606/4202466‑4202610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑