Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,305,392	T→C	E283G (GAA→GGA)	cls ←	cardiolipin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,305,392	0	T	C	100.0%	119.8 / NA	30	E283G (GAA→GGA)	cls	cardiolipin synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/16);  total (14/16)

TGTGACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCAT  >  REL606/1305321‑1305459                                                                         |                                                                    catgACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAAt                                                                 >  2:1168281/3‑76 (MQ=255)   tgACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAAtct                                                               >  1:1022898/1‑76 (MQ=255)    gACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAAtctc                                                              >  1:129030/1‑76 (MQ=255)        gCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAg                                                          >  1:531410/1‑76 (MQ=255)         cTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGt                                                         >  1:1373972/1‑76 (MQ=255)          tGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTc                                                        <  1:461653/76‑1 (MQ=255)           ggCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCg                                                       >  2:17538/1‑76 (MQ=255)           ggCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCg                                                       >  2:1186995/1‑76 (MQ=255)           ggCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCg                                                       >  2:1038785/1‑76 (MQ=255)              cTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAg                                                    >  2:1178647/1‑76 (MQ=255)                   cAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGaata                                               >  1:1079948/1‑76 (MQ=255)                   cAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGaata                                               >  1:460073/1‑76 (MQ=255)                     aaCGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGaataaa                                             <  2:731463/76‑1 (MQ=255)                      aCGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGaataaat                                            <  2:1613027/76‑1 (MQ=255)                        ggCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAataaataa                                          <  1:333179/76‑1 (MQ=255)                              atatTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCa                                    >  2:682871/1‑76 (MQ=255)                              atatTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCa                                    <  1:443653/76‑1 (MQ=255)                                 ttGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCATcg                                 <  1:1178647/76‑1 (MQ=255)                                   gACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCATcgcg                               >  1:23891/1‑76 (MQ=255)                                   gACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCATcgcg                               <  1:211997/76‑1 (MQ=255)                                    aCATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCgg                              <  2:438305/76‑1 (MQ=255)                                       tCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTgg                           <  2:318434/76‑1 (MQ=255)                                         tggtggtggCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCg                         <  2:833377/76‑1 (MQ=255)                                           gtggtggCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGAt                       <  1:932948/76‑1 (MQ=255)                                             ggtggCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATgg                     <  2:1157681/76‑1 (MQ=255)                                              gtggCGGCAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCCGGGGCGATggg                    >  1:794347/1‑76 (MQ=255)                                                 gcggcAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGcc                 <  2:1621619/76‑1 (MQ=255)                                                 gcggcAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGcc                 <  2:412331/76‑1 (MQ=255)                                                 gcggcAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGcc                 <  2:830275/76‑1 (MQ=255)                                                  cggcAGAATACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGCCt                <  2:1200101/76‑1 (MQ=255)                                                   ggcAGAATACGTTTTCC‑GGTTCCAATCCCCCAGCCGCAGAAATAAATAACCCCCATCGCGGGGGCGATGGGGCCtt               <  2:995049/76‑1 (MQ=255)                                                        aaTACGTTTTCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGCCTTCCATa          >  2:102644/1‑76 (MQ=255)                                                           tccttttCCGGGTTCCAACCTCCCAGTGGCAGGAATAAAAAATCCCCACGGGGGGGGGGAGGGGGCCTTCCATAcg        <  2:1388620/73‑1 (MQ=255)                                                              ttttCC‑GGTTCCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGcc    >  1:939938/1‑76 (MQ=255)                                                                tccc‑GGTCCCAATCCCCCAGTCGCAGGAATAAATAACCCCCATCGCGGGGGGGATGGGGCCTTCCATACGCGCCAt  <  2:1360132/74‑1 (MQ=255)                                                                         |                                                                    TGTGACCGCTGGCCTGTTCAAACGGCATAATATTGACATCTGGTGGTGGCGGCAGAATACGTTTTCC‑GGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATGGGGCCTTCCATACGCGCCAT  >  REL606/1305321‑1305459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑