| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,911,964 | 2 bp→TT | intergenic (‑165/‑157) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,911,964 | 0 | A | T | 95.2% | 156.2 / ‑0.8 | 42 | intergenic (‑165/‑158) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base T (16/24); total (17/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
| * | REL606 | 3,911,965 | 0 | A | T | 95.2% | 157.7 / ‑0.7 | 42 | intergenic (‑166/‑157) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base T (16/24); total (17/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
TGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAA > REL606/3911894‑3912034 || tgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGtttttt < 2:1112164/76‑1 (MQ=255) cGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTtctcc > 1:209137/1‑73 (MQ=255) aaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTcactcact < 1:796638/76‑1 (MQ=255) aaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACta > 2:402873/1‑76 (MQ=255) aTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACtatt > 1:1234795/1‑76 (MQ=255) cAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACtatttta > 1:413494/1‑76 (MQ=255) ggACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTattttatttt > 1:823162/1‑76 (MQ=255) gACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTAttttt < 1:1447913/76‑1 (MQ=255) aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTaaa < 1:150552/76‑1 (MQ=255) ggATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTaaaaaa > 2:1203783/1‑76 (MQ=255) ggATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTaaaaaa > 1:1049317/1‑76 (MQ=255) gATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAaac < 2:1490244/76‑1 (MQ=255) aTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAaaca < 2:103899/76‑1 (MQ=255) tGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAaacaa < 2:1247413/76‑1 (MQ=255) tGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAaacaa < 1:665505/76‑1 (MQ=255) gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAaacaac < 1:1547395/76‑1 (MQ=255) aCTACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAAcaacaa < 1:754284/76‑1 (MQ=255) tACGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAtt < 1:306521/76‑1 (MQ=255) aCGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAttt < 1:926820/76‑1 (MQ=255) aCGAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAttt < 1:56330/76‑1 (MQ=255) gAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat < 1:730472/76‑1 (MQ=255) gAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat < 2:345745/76‑1 (MQ=255) gAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat < 2:678853/76‑1 (MQ=255) gAGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat < 1:1111426/76‑1 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtata < 1:1047284/76‑1 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtata < 2:1060550/76‑1 (MQ=255) tgAAGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTatat > 2:1140690/2‑76 (MQ=255) aaGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg > 1:885167/1‑76 (MQ=255) aaGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg < 2:503207/76‑1 (MQ=255) aaGGGAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg > 1:829107/1‑76 (MQ=255) gggAACAACATTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaa > 2:1612752/1‑76 (MQ=255) acaacaTTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatta > 1:1335138/1‑76 (MQ=255) aacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAattatt > 2:366857/1‑76 (MQ=255) caTTCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaa < 1:237968/76‑1 (MQ=255) caTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaa < 2:553993/76‑1 (MQ=255) ttCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAAc < 2:1399482/76‑1 (MQ=255) ttCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAAc > 2:706634/1‑76 (MQ=255) tCATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACg < 1:956394/76‑1 (MQ=255) cATACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGc < 1:1154509/76‑1 (MQ=255) tACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcat > 1:964614/1‑76 (MQ=255) tACTGAAATTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcat > 1:1383434/1‑76 (MQ=255) aaaTTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa > 2:209415/1‑76 (MQ=255) aaTTGTTTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 2:1324657/76‑1 (MQ=255) || TGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAA > REL606/3911894‑3912034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |