| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,875,625 | 1 | . | T | 91.9% | 219.1 / 10.5 | 62 | intergenic (‑59/+294) | glmU/atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Reads supporting (aligned to +/- strand): ref base . (2/3); new base T (30/27); total (32/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑T‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAG > REL606/3875552‑3875691 | aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t‑t < 1:634045/76‑1 (MQ=255) gCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t‑t‑t < 2:1898896/76‑1 (MQ=255) gCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t‑t‑t < 2:829947/76‑1 (MQ=255) ttatcCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t‑t‑t‑t‑t‑t > 1:1880082/1‑76 (MQ=255) tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t‑t‑t‑t‑t‑tt > 2:1539305/1‑76 (MQ=255) ttCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t‑t‑t‑t‑t‑tttc > 1:416733/1‑74 (MQ=255) aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTg < 2:846339/76‑1 (MQ=255) gcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAAt < 2:839211/76‑1 (MQ=255) gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATgg > 1:871474/1‑76 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAATCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGAc < 2:678117/76‑1 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCGCCTTGAAAAAACTACA‑T‑T‑TAT‑T‑TT‑CATCGTGAAGTgggc < 2:1971321/76‑3 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATGGTGAAATGGAc < 1:68454/76‑1 (MQ=255) ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGAc < 2:1391776/76‑1 (MQ=255) cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACa < 2:1038915/76‑1 (MQ=255) tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACag > 1:630523/1‑76 (MQ=255) tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGGGAAATGGACag < 1:50776/76‑1 (MQ=255) gACTGTAATTAGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATTGTGAAATGGACaga > 1:169440/1‑76 (MQ=255) gTAATTTGAGAANGANTTTAAACCGCTTCACCTTNAAAAAAATACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGAt < 1:1665932/76‑1 (MQ=255) gTAATTTGAGAACGAATTTCAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGAt > 1:647291/1‑76 (MQ=255) gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGAt > 1:230586/1‑76 (MQ=255) gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGAt < 1:861498/76‑1 (MQ=255) tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGAtn < 1:1543004/76‑2 (MQ=255) aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATaa > 2:1077671/1‑76 (MQ=255) tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAAt < 1:1005006/76‑1 (MQ=255) tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAAt > 1:1026807/1‑76 (MQ=255) ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACG‑TAT‑T‑T‑T‑TA‑CAACGTGAAATGGAGAGANGATAAAtt > 2:341396/1‑76 (MQ=255) ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAAtt < 1:497729/76‑1 (MQ=255) tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTg < 1:976477/76‑1 (MQ=255) aaCGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCa < 1:877572/76‑1 (MQ=255) aaCGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCa < 1:1531277/76‑1 (MQ=255) cGAATTTAAAGCGCTTCGCCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGTATCAATTGTTCAAt > 1:163009/1‑76 (MQ=255) gAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATa > 1:1053373/1‑76 (MQ=255) gAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATa < 1:1240991/76‑1 (MQ=255) tttAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg < 1:596199/76‑1 (MQ=255) tttAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg > 2:1417176/1‑76 (MQ=255) ttAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg < 2:1772500/76‑1 (MQ=255) ttAAACCGCTTCACCTTGAAAAAACTACA‑T‑T‑T‑T‑T‑TTCCATCGTGAAATGGACAGAAGATAAATTGTTCAATAACgg > 2:1632389/1‑76 (MQ=255) tAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 1:809015/1‑76 (MQ=255) tAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 1:1417586/1‑76 (MQ=255) tAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 1:1491446/1‑76 (MQ=255) aaaCCGCTTCACCTTGAAACAACTACA‑T‑TAT‑T‑T‑AT‑CATAGTGAAGTGGGTAGAGGATAAATTGTTCACTAACAGAt > 1:1972448/1‑76 (MQ=255) aaCCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 1:1016583/76‑1 (MQ=255) aaCCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt > 2:1281194/1‑76 (MQ=255) aaCCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 2:209639/76‑1 (MQ=255) aaCCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 2:1901768/76‑1 (MQ=255) aaCCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 1:609603/76‑1 (MQ=255) aaCCGCTTCACCTTGAAAAAACTACA‑T‑T‑T‑TAT‑TT‑CATCGCGAAATGGACACAGGATAAATTGTTCAATAACGGAtt < 1:26951/76‑1 (MQ=255) aCCGCTTCATCGTGAAAAAACTACA‑T‑T‑T‑T‑T‑TTGCATCGTGAAATGGACAGAGGATAGCGTGTTCAATAACGGATTa < 1:23726/76‑1 (MQ=255) aCCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTa < 2:920401/76‑1 (MQ=255) ccGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCATGAAATGGTCAGAGGATAAATTGTTCAATAACGGATTAt > 1:38458/1‑76 (MQ=255) cGCTTAACATTGAANAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATc > 1:136276/1‑76 (MQ=255) cTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATccc > 1:1384928/1‑76 (MQ=255) ttCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc > 1:703512/1‑76 (MQ=255) ttCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc < 2:511534/76‑1 (MQ=255) gtCACCTTGAAAAAACTACA‑T‑T‑T‑T‑TCTT‑TATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc < 1:325553/75‑1 (MQ=255) tCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg > 1:1206011/1‑76 (MQ=255) tCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg > 2:1816671/1‑76 (MQ=255) aCCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGGTCAATAACGGATTATCCCCGCt < 2:802260/76‑1 (MQ=255) ccTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg < 1:1429627/76‑1 (MQ=255) ccTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg < 2:546952/76‑1 (MQ=255) cTTGAAAAAACTACNTT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGAAAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa > 1:5954/1‑76 (MQ=255) cTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa > 1:145421/1‑76 (MQ=255) cTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa > 2:627906/1‑76 (MQ=255) tGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGCTTATCCCCGCTGACg > 1:1694618/1‑76 (MQ=255) gAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGa > 1:1046227/1‑76 (MQ=255) aaaaaaCTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCACACACGGATTATCCCCGCTGACGaa > 2:1421227/1‑76 (MQ=255) aaaaaaCTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa > 2:1956427/1‑76 (MQ=255) aaaaaaCTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa > 1:898438/1‑76 (MQ=255) aaaaaCTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa > 2:689269/1‑76 (MQ=255) aaaaCTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg > 2:1042375/1‑76 (MQ=255) | AGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑T‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAG > REL606/3875552‑3875691 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |