| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 474,424 | A→T | D294E (GAT→GAA) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 474,424 | 0 | A | T | 97.9% | 166.3 / NA | 48 | D294E (GAT→GAA) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (26/21); minor base C (1/0); total (27/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCG > REL606/474353‑474496 | aaCGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCaa > 2:1860398/1‑76 (MQ=255)aaCGACATCCCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCaa > 1:1899020/1‑76 (MQ=255) aCGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCaaa > 2:707294/1‑76 (MQ=255) cGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAaac < 1:1104319/76‑1 (MQ=255) cGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAaac < 1:1104338/76‑1 (MQ=255) aCCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATc > 1:367638/1‑76 (MQ=255) cAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATccc < 1:1217863/76‑1 (MQ=255) aaaCAGANAAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCg > 2:1642880/1‑76 (MQ=255) aaaCAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCg < 2:588893/76‑1 (MQ=255) aaaCAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCg > 2:1319189/1‑76 (MQ=255) aaaCAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATGAACGGGTCAAACAACATCCCg > 2:1223804/1‑76 (MQ=255) aCAGAATAAGCGCCAGCGTCGCCAGCACTGCCAGCAGTTCCTGAATCAGAATTAACGGTTCAAACAACATCCCGAc < 2:1633054/76‑1 (MQ=255) aCAAAATGAGCGCCAGCGGCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGAc < 2:821121/76‑1 (MQ=255) aGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACgg > 1:1413048/1‑76 (MQ=255) gAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGga < 1:759964/76‑1 (MQ=255) aataatCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGgag < 1:870839/76‑1 (MQ=255) atCGCCGGCATCGCCAGCGCTGCCAGCGGTTGCTGAATCAGAATTAACGATTCAAACAACANCCNGACGGAGACaa > 2:311702/1‑76 (MQ=255) tCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACaaa > 2:1464511/1‑76 (MQ=255) cGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACaaaa < 1:694511/76‑1 (MQ=255) cAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAAc < 1:407659/76‑1 (MQ=255) cAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAAc < 1:1395792/76‑1 (MQ=255) cAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAAc < 2:1131335/76‑1 (MQ=255) cAGCGTCGCCAGCACTGCCAGCGGTGGCCGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAAc < 1:319440/76‑1 (MQ=255) aGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCTAAGAACATCCCGACGGAGACAAAAAACa > 1:314542/1‑76 (MQ=255) aGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACa > 1:620407/1‑76 (MQ=255) gTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCa > 1:860524/1‑76 (MQ=255) cGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCAcc > 2:1562590/1‑76 (MQ=255) cAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCa > 1:655080/1‑76 (MQ=255) aGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCaa > 2:1087471/1‑76 (MQ=255) cACTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAAc < 1:767095/76‑1 (MQ=255) cTGCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGAACCGCAAAcgc > 1:230326/1‑76 (MQ=255) gCCAGCGGTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGt > 2:1356451/1‑76 (MQ=255) ggTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCa < 2:1437727/76‑1 (MQ=255) ggTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCa > 1:367260/1‑76 (MQ=255) gTTGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCaa > 1:887864/1‑76 (MQ=255) tGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATg > 1:1044701/1‑76 (MQ=255) tGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATg < 1:1703842/76‑1 (MQ=255) tGCTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATg > 1:719159/1‑76 (MQ=255) cTGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGc < 2:1044700/76‑1 (MQ=255) tGAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCa < 1:1228712/76‑1 (MQ=255) gAATCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAg > 1:1556567/1‑76 (MQ=255) aaTCAGAATTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGc < 1:1857623/76‑1 (MQ=255) aGAATTAACGGNTCNCACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGCCGCGCAATCGCAGCGTAt < 2:314515/76‑1 (MQ=255) aTTAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGt < 1:604073/76‑1 (MQ=255) ttAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCACACCCGTCGCGCAATGGCAGCGTATCGTg > 1:321865/1‑76 (MQ=255) ttAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTg > 1:1336733/1‑76 (MQ=255) tAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTgg > 1:1538414/1‑76 (MQ=255) tAACGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTgg < 2:632803/76‑1 (MQ=255) tAACGGCTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTgg > 1:1064185/1‑76 (MQ=255) cGGTTCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGggcg > 2:981980/1‑76 (MQ=255) | AACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCG > REL606/474353‑474496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |