BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,166,492	A→C	Y511S (TAC→TCC)	rpoC →	DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,166,492	0	A	C	100.0%	231.9 / NA	57	Y511S (TAC→TCC)	rpoC	DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (29/28); total (29/28)

ACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCA  >  REL606/4166419‑4166559

aCCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGtcct                                                                   >  2:888382/1‑76 (MQ=255)

aCCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGtcct                                                                   >  1:793592/1‑76 (MQ=255)

aCCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGtcct                                                                   <  1:733489/76‑1 (MQ=255)

aCCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGtcct                                                                   >  2:1433832/1‑76 (MQ=255)

aCCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGtcct                                                                   <  2:1788923/76‑1 (MQ=255)

 ccAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGtccta                                                                  <  2:1966875/76‑1 (MQ=255)

  caacaaCATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGtactac                                                                 >  2:428133/1‑76 (MQ=255)

         agccTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGAccc                                                          >  2:1974037/3‑76 (MQ=255)

         aTCCTGTCCCCGGCGAACGGCGAACCAGTCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGAccc                                                          <  2:1147737/76‑1 (MQ=255)

         aTCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGAccc                                                          <  2:1147733/76‑1 (MQ=255)

         aTCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGAccc                                                          >  2:727001/1‑76 (MQ=255)

           ccTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGt                                                        >  2:279493/1‑76 (MQ=255)

            cTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTg                                                       >  1:1269900/1‑76 (MQ=255)

              gTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGAc                                                     >  2:787091/1‑76 (MQ=255)

              gTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGAc                                                     >  2:1372606/1‑76 (MQ=255)

              gTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGAc                                                     >  2:257667/1‑76 (MQ=255)

              gTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGAc                                                     >  2:1004441/1‑76 (MQ=255)

               tCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACTCGTGACt                                                    >  2:1858930/1‑76 (MQ=255)

                ccccGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACtg                                                   >  1:1486516/1‑76 (MQ=255)

                 cccGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACtgt                                                  <  2:1573620/76‑1 (MQ=255)

                  ccGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACtgtg                                                 <  2:1790623/76‑1 (MQ=255)

                   cggcgaacggcgaaCCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTgtgt                                                <  2:1252824/76‑1 (MQ=255)

                    ggcgaacggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGtt                                               <  2:794691/76‑1 (MQ=255)

                    ggcgaacggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGtt                                               <  2:1199909/76‑1 (MQ=255)

                     gcgaacggcgaagCCATCACCGTTCCGTCGCAGGACGTTGTACTGGGTCTGTCCAACATGACCCGTGTCTGTGTTa                                              <  2:32361/76‑1 (MQ=255)

                      cgaacggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTaa                                             <  2:260917/76‑1 (MQ=255)

                           ggcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCa                                        >  1:970639/1‑76 (MQ=255)

                            gcgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCaa                                       <  1:1340252/76‑1 (MQ=255)

                             cgaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCaaa                                      <  1:607813/76‑1 (MQ=255)

                              gaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAg                                     >  1:285033/1‑76 (MQ=255)

                              gaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAg                                     <  2:1544489/76‑1 (MQ=255)

                              gaacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCACACCTACCTGACCCGTGACTGTCTTANCGCCAAAg                                     >  2:341144/1‑76 (MQ=255)

                               aacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAgg                                    >  2:758000/1‑76 (MQ=255)

                               aacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAgg                                    >  2:1682167/1‑76 (MQ=255)

                               aacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAgg                                    >  2:1810001/1‑76 (MQ=255)

                               aacCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAgg                                    >  1:1422727/1‑76 (MQ=255)

                                  cAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGa                                 <  2:1452155/76‑1 (MQ=255)

                                    atcatcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAg                               <  2:1377528/76‑1 (MQ=255)

                                    atcatcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAg                               <  1:1550384/76‑1 (MQ=255)

                                     tcatcGTTCCGTCTNAGGACGTTGTACTGGGTCTGTCCTACAAGACCCGTGACTGTGTTAACGGCAAAGGCGAAgg                              <  1:1752741/76‑1 (MQ=255)

                                        tcGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCAt                           <  1:893486/76‑1 (MQ=255)

                                         cGTTCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATg                          <  1:1144610/76‑1 (MQ=255)

                                            tCCGTCTCAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTg                       <  1:1502156/76‑1 (MQ=255)

                                                 ctcAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGAc                  >  1:1569807/1‑76 (MQ=255)

                                                 ctcAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGAc                  >  1:560207/1‑76 (MQ=255)

                                                  tcAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTACCGCCAAAGGCGAAGGCATGGTGCTGACt                 >  2:794064/1‑76 (MQ=255)

                                                  tcAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAGAGGCGAAGGCATGGTGCTGACt                 <  1:1301770/76‑1 (MQ=255)

                                                  tcAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACt                 <  1:1231497/76‑1 (MQ=255)

                                                   cAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTg                <  1:1227436/76‑1 (MQ=255)

                                                   cAGGACGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTg                >  2:1402283/1‑76 (MQ=255)

                                                        cGTTGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCg           <  1:813110/76‑1 (MQ=255)

                                                          ttGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGaa         <  2:1533682/76‑1 (MQ=255)

                                                          ttGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGaa         >  2:1154922/1‑76 (MQ=255)

                                                           tGTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGaaa        <  1:533490/76‑1 (MQ=255)

                                                            gTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAaag       >  2:859631/1‑76 (MQ=255)

                                                            gTACTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAaag       <  1:1480001/76‑1 (MQ=255)

                                                             tACTGGGTCTGTCCTACATGACCCGTGACTGTATTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAaaga      >  2:1533057/1‑76 (MQ=255)

                                                              aCTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGNCATGGTGCTGACTGGCCCGAaagaa     >  2:136852/1‑76 (MQ=255)

                                                              aCTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAaagaa     >  1:938639/1‑76 (MQ=255)

                                                               cTGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAaagaag    <  1:339667/76‑1 (MQ=255)

                                                                tGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGc   <  1:1160314/76‑1 (MQ=255)

                                                                tGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGc   >  1:233127/1‑76 (MQ=255)

                                                                tGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGc   >  2:1424993/1‑76 (MQ=255)

                                                                tGGGTCTGTCCTACATGACCCGTGACTGTGTTAACGCC‑AAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCa  >  1:349605/1‑76 (MQ=255)

                                                                tGGGTCTGTCATACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGc   >  1:160081/1‑76 (MQ=255)

ACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACTGTGTTAACGCCAAAGGCGAAGGCATGGTGCTGACTGGCCCGAAAGAAGCA  >  REL606/4166419‑4166559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`