| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,438,531 | T→G | D110A (GAC→GCC) | fbp ← | fructose‑1,6‑bisphosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,438,531 | 0 | T | G | 97.8% | 166.4 / ‑6.3 | 45 | D110A (GAC→GCC) | fbp | fructose‑1,6‑bisphosphatase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (19/25); minor base A (0/1); total (19/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
AACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAA > REL606/4438458‑4438603 | aaCGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGcc > 1:709995/1‑76 (MQ=255)aaCGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGcc > 2:572690/1‑76 (MQ=255) aCGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCa > 2:1239537/1‑76 (MQ=255) cggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGCNGCCATCa < 2:1648258/76‑1 (MQ=255) tAGATGGAGAAAATGGTNACGACAGAGACGTTAACATCGATGTTGGACGAGCTATCCAGGGGGGCCGTCAGcacca > 1:258796/1‑76 (MQ=255) tAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcacca > 2:1074359/1‑76 (MQ=255) tAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcacca > 2:637998/1‑76 (MQ=255) gagaAAATGGTNCCNACAGAGACGTTCACATCGATGTTGGACGAGCCATCCAGGGGGATCATCAGCTCCCCGTAtt < 2:325070/76‑1 (MQ=255) gaAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCGNGTAtttt < 2:1652830/76‑1 (MQ=255) ggTACCGACAGCGACCTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCgtgt > 1:61572/1‑76 (MQ=255) cccGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTca < 2:1294318/75‑1 (MQ=255) ccGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcac < 2:956105/76‑1 (MQ=255) cGACAGAGACGTTAACATCGTTGTTGGACGAGCCATCCAGGGGGGCCATCAGCGCCACGTATTTTGCGTGTTcaca > 2:167904/1‑76 (MQ=255) cGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcaca < 2:495763/76‑1 (MQ=255) gACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAg < 2:1600611/76‑1 (MQ=255) gACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGAGCCATCAGCACCACGTATTTTCCGTGTTCagag < 1:1867716/76‑1 (MQ=255) aCAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGc > 1:1103327/1‑76 (MQ=255) aCAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGc > 2:394534/1‑76 (MQ=255) cAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGcc > 2:1596101/1‑76 (MQ=255) agagACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCt > 1:964634/1‑76 (MQ=255) agagACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCt > 2:1022367/1‑76 (MQ=255) agagACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCt > 2:746774/1‑76 (MQ=255) gagaCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCtt < 1:1264321/76‑1 (MQ=255) aCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaa < 1:862572/76‑1 (MQ=255) aCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaa < 1:1150875/76‑1 (MQ=255) aCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGCGTTCACAGCCTTCaa > 1:1687308/1‑76 (MQ=255) aTCGATGTTGGANGANCCATCCAGGGGGGCCATCNGCACCATGTATTTTGCGTGTTCACAGCCTTCAAAGACGACa < 1:1669452/76‑1 (MQ=255) cGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAAt < 1:746775/76‑1 (MQ=255) aTGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAAtct > 2:1481302/1‑76 (MQ=255) aTGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGACTTCAAAGACGACAAtct < 1:1925088/76‑1 (MQ=255) ttGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAt < 2:556139/76‑1 (MQ=255) ttGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAt < 1:1142859/76‑1 (MQ=255) ttGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAt < 1:1339249/76‑1 (MQ=255) ttGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGAGTTCACAGCCTTCAACGACGACAATCTCAt > 1:43385/1‑76 (MQ=255) tGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtc > 2:677952/1‑76 (MQ=255) gAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttctt < 2:1584976/76‑1 (MQ=255) gAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttctt > 2:1071652/1‑76 (MQ=255) gAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttctt > 2:476181/1‑76 (MQ=255) ccATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATcttcttctt < 2:1450621/76‑1 (MQ=255) ccATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATcttcttctt < 2:1450603/76‑1 (MQ=255) aTCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCa > 1:1937175/1‑76 (MQ=255) ccAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCaga < 1:1503021/76‑1 (MQ=255) ccAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCaga < 1:1476445/76‑1 (MQ=255) cAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCagag < 1:838573/76‑1 (MQ=255) aGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAgg < 2:1299542/76‑1 (MQ=255) ggggggCCATCAGCCCCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCGTCTTCTCCTTCAGAGGc < 1:168935/76‑1 (MQ=255) ggggGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCa < 1:1703940/76‑1 (MQ=255) ggggGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCa < 1:1425147/76‑1 (MQ=255) ggggCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCaa > 2:980975/1‑76 (MQ=255) ggggCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCaa > 2:629529/1‑76 (MQ=255) ggggCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCaa > 2:495473/1‑76 (MQ=255) | AACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAA > REL606/4438458‑4438603 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |