Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,329,516	0	C	T	100.0%	146.5 / NA	40	H33Y (CAC→TAC)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/15);  total (25/15)

TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329443‑1329588                                                                          |                                                                         tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtac                                                                        <  1:1209782/76‑1 (MQ=255)   aGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACAt                                                                      >  2:744686/1‑76 (MQ=255)     tCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATcc                                                                    <  2:1742449/76‑1 (MQ=255)      ccccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcg                                                                   >  2:1938249/1‑76 (MQ=255)         cGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGa                                                                >  1:459242/1‑76 (MQ=255)          ggCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAt                                                               <  2:724713/76‑1 (MQ=255)             aaaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTg                                                            >  1:889383/1‑76 (MQ=255)             aaaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTg                                                            >  2:1091259/1‑76 (MQ=255)              aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGc                                                           <  2:1821049/76‑1 (MQ=255)               aaGCCAAAGCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGcc                                                          >  1:1901572/1‑76 (MQ=255)                aGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCg                                                         <  1:217919/76‑1 (MQ=255)                  ccAAAACGATCAACAAGTATCTGGCTAGNTACTACGTGGTGAAATCCAGCNTCGGTTACATCCGCGATTTGCCGAc                                                       >  1:300650/1‑76 (MQ=255)                   cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAcc                                                      >  2:934200/1‑76 (MQ=255)                      aaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGt                                                   <  1:818935/76‑1 (MQ=255)                         gATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGc                                                >  1:1490361/1‑76 (MQ=255)                         gATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGc                                                >  1:574107/1‑76 (MQ=255)                          aTCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCt                                               >  1:283870/1‑76 (MQ=255)                          aTCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCt                                               <  2:1171623/76‑1 (MQ=255)                           tCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTc                                              >  2:802213/1‑76 (MQ=255)                           tCAACAAGTAGCTGGGTGGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTCCCGACCAGTGGCTc                                              >  1:158921/1‑76 (MQ=255)                             aacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAg                                            >  1:604818/1‑76 (MQ=255)                               caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                          <  1:1022219/76‑1 (MQ=255)                                    aTCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaa                                     <  1:1091260/76‑1 (MQ=255)                                      cTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaaa                                   >  1:120092/1‑76 (MQ=255)                                          gTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAagag                               >  2:974448/1‑76 (MQ=255)                                           tAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCTCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGt                              <  1:1716636/76‑1 (MQ=255)                                            aGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTg                             >  1:331457/1‑76 (MQ=255)                                            aGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTg                             >  1:1219405/1‑76 (MQ=255)                                                 cTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAc                        >  1:1437994/1‑76 (MQ=255)                                                  tACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAct                       <  2:1038093/76‑1 (MQ=255)                                                    cGTGGTGAAATCCAGCGTGAGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGNCGActct                     <  2:1642923/76‑1 (MQ=255)                                                    cGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGNCGActct                     <  2:1642897/76‑1 (MQ=255)                                                    cGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActct                     <  2:62101/76‑1 (MQ=255)                                                     gtggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTa                    >  2:1064504/1‑76 (MQ=255)                                                      tggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTAc                   <  2:1343024/76‑1 (MQ=255)                                                            aaTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccac             >  2:982324/1‑76 (MQ=255)                                                             aTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTTGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacc            <  1:1693184/76‑1 (MQ=255)                                                             aTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacc            >  1:1224830/1‑76 (MQ=255)                                                                caccGTCGGTTACATCCGAGATATGCCNACNATNGGTTCAGNTGGCAACAAGAGTGCCGACTCTACCTCCACCAAg         <  1:1763431/73‑1 (MQ=255)                                                                cAGCGTCGGTTACATCCGCGAATTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCCAg         >  2:1565273/1‑76 (MQ=255)                                                                 aGCGTCGGTTACATCCGCGATNTGNCGACCAGCGGCTCAGCTGCCANAAAGAGTNCNGACTCTACCTCCACCAAGa        >  2:292175/1‑76 (MQ=255)                                                                   cGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACg      >  1:853614/1‑76 (MQ=255)                                                                      cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCt   <  2:1397546/76‑1 (MQ=255)                                                                      cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCt   <  2:768342/76‑1 (MQ=255)                                                                      cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCt   >  1:1576898/1‑76 (MQ=255)                                                                       ggTTACATCCGCGATTTGCCGAGCANTCGCTCAGCTCCCAAAAAGAANGCCAACTCTACCTCCACCAAGACGGCTa  <  1:293209/76‑1 (MQ=255)                                                                          |                                                                         TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329443‑1329588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑