Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	170.9 / NA	53	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (30/23);  total (30/23)

CGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193708‑2193850                                                                         |                                                                        cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                                                                     >  2:507720/1‑76 (MQ=255)    tGCATCCGGCGTCCGGGAGCGGGTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc                                                                  <  1:103938/76‑1 (MQ=255)     gCAGCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca                                                                 <  2:973346/76‑1 (MQ=255)      cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg                                                                >  1:984506/1‑76 (MQ=255)      cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg                                                                >  1:1106524/1‑76 (MQ=255)       aTCCGGCGTCCAGGCGCGGCTGGACTGCCTGTCGGGAAAGCA‑CCCCGATTAAAGCGAAACGCCAATACCCGCCAGa                                                               >  2:799197/1‑76 (MQ=255)        tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                              >  2:840020/1‑76 (MQ=255)        tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGGC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCCGAt                                                              >  2:1436704/1‑76 (MQ=255)          cGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                            >  2:1762287/1‑76 (MQ=255)           ggCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                           <  1:1342144/76‑1 (MQ=255)           ggCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                           >  1:1504416/1‑76 (MQ=255)              gTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa                                                        >  2:1590975/1‑76 (MQ=255)                ccAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                      <  2:729661/76‑1 (MQ=255)                ccAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                      <  1:1001507/76‑1 (MQ=255)                  aGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc                                                    >  2:1410922/1‑76 (MQ=255)                  aGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc                                                    <  1:749892/76‑1 (MQ=255)                      gcgGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt                                                <  1:1433984/76‑1 (MQ=255)                       cgGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                               <  1:665371/76‑1 (MQ=255)                       cgGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                               <  2:688370/76‑1 (MQ=255)                       cgGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                               >  2:1788031/1‑76 (MQ=255)                          cTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                            <  1:1854857/76‑1 (MQ=255)                          cTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                            >  2:1316994/1‑76 (MQ=255)                           tGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                           <  1:553942/76‑1 (MQ=255)                             gTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGATGCCTGTATTaa                                         <  1:116932/76‑1 (MQ=255)                              tCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                        <  1:1948854/76‑1 (MQ=255)                                tGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                      >  1:1310084/1‑76 (MQ=255)                                tGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                      >  2:143193/1‑76 (MQ=255)                                tGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                      >  1:524875/1‑76 (MQ=255)                                  gCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg                                    <  2:1840777/76‑1 (MQ=255)                                  gCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg                                    >  2:1868645/1‑76 (MQ=255)                                    tGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                  >  1:1597005/1‑76 (MQ=255)                                     gTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                 <  2:1493108/76‑1 (MQ=255)                                      tC‑GGAAATCAGCCCCAATAACAGCAAATCGCTAANCCCCGCCNCANACNGCATAACGCCTGTATTAAACAGGCttt                                >  1:1768119/1‑76 (MQ=255)                                      tC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                >  2:781495/1‑76 (MQ=255)                                       c‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                               >  1:339549/1‑76 (MQ=255)                                         ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                              <  2:1809280/76‑1 (MQ=255)                                          gAAATCAGCCCCAATAACAGCAAACCGCNGATCCCCACCAGATAAAGCTTNACGCCTGTATTAAACAGGCTTTTTa                             >  1:293433/1‑76 (MQ=255)                                           aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                            >  2:1262864/1‑76 (MQ=255)                                            aaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                           >  2:781176/1‑76 (MQ=255)                                             aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                          >  2:434834/1‑76 (MQ=255)                                               cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                        >  1:1863999/1‑76 (MQ=255)                                                        tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg               >  2:1403930/1‑76 (MQ=255)                                                        tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg               <  2:1975108/76‑1 (MQ=255)                                                        tAACAGCAAAACGCCAATCACCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAAGGCGCATcg               >  2:131725/1‑76 (MQ=255)                                                              cAAAACGCCAATCCCCACCNGNTCAAGCANGACGCCTGTATTACACAGGCTNTTNACAGATGGCGCATCGCGCAcc         <  2:293433/76‑1 (MQ=255)                                                              cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGTCTTTTTACCGATGGCGCATCGCGCAcc         >  1:214449/1‑76 (MQ=255)                                                              cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc         <  1:1474225/76‑1 (MQ=255)                                                              cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGACGGCGCATCGCGCAcc         >  1:1695574/1‑76 (MQ=255)                                                               aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa        >  1:1230124/1‑76 (MQ=255)                                                                aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg       <  1:1625983/76‑1 (MQ=255)                                                                 aaCGCCAATCCCCACCAGCTAAAGCATNACNCCNGTCTTAANCATGCTCTTTACCGATGGCGCATCGCGCACCAgc      <  1:1762288/76‑1 (MQ=255)                                                                 aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc      <  1:1026210/76‑1 (MQ=255)                                                                  aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc     >  2:1288106/1‑76 (MQ=255)                                                                     ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc  <  2:1519940/76‑1 (MQ=255)                                                                     ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc  >  1:279664/1‑76 (MQ=255)                                                                         |                                                                        CGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193708‑2193850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑