| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,145,869 | G→T | E2D (GAG→GAT) | rpoD → | RNA polymerase sigma factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,145,869 | 0 | G | T | 98.0% | 174.0 / ‑2.9 | 51 | E2D (GAG→GAT) | rpoD | RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (25/24); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01 | |||||||||||
AACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCTATGCCGAG > REL606/3145797‑3145941 | aaCGGCTGAAGGACATCGGGTCAATCGCCCAACACCACCCTCATGAAATAAGTGTGGATACCGTCTTATGGATCaa > 1:1714532/1‑76 (MQ=255)aaCGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCaa > 1:1118789/1‑76 (MQ=255) aCGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATGAGTGTGGATACCGTCTTATGGATCaaa < 1:1812560/76‑1 (MQ=255) cGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCaaaa < 1:970937/76‑1 (MQ=255) cGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCaaaa > 1:969798/1‑76 (MQ=255) cTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAAccc > 2:1125814/1‑76 (MQ=255) cTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAAccc < 1:869618/76‑1 (MQ=255) cTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAAccc < 1:1941276/76‑1 (MQ=255) gAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAGATAAGTGTNGATACCGTCTTATGGATCAAAACCCGc < 1:1802745/76‑1 (MQ=255) gAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGc > 1:1023194/1‑76 (MQ=255) gAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGc > 1:643640/1‑76 (MQ=255) gAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGc > 2:506975/1‑76 (MQ=255) aaGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGCa > 2:1751454/1‑76 (MQ=255) aaGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGCa > 2:544802/1‑76 (MQ=255) gACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAACACCCGCAGTc < 1:1947122/76‑1 (MQ=255) gACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGCAGTc < 2:1755201/76‑1 (MQ=255) aCATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGCAGTca < 2:471841/76‑1 (MQ=255) tCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGCAGTCACAg < 2:700501/76‑1 (MQ=255) gggTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGCAGTCACAGCt < 2:1130067/76‑1 (MQ=255) aaTCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAAc > 2:373190/1‑76 (MQ=255) cGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCAGAGCTGAAActtc > 1:787266/1‑76 (MQ=255) ccAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTg > 2:412697/1‑76 (MQ=255) cAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGt > 1:485600/1‑76 (MQ=255) aaCACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTc > 1:1323818/1‑76 (MQ=255) ccTCATGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTgg < 1:1534943/76‑1 (MQ=255) cATGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTaa < 1:1273290/76‑1 (MQ=255) cATGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTaa < 1:1442447/76‑1 (MQ=255) aTGAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAg < 1:837214/76‑1 (MQ=255) tGAAATAAGTGTGGATANCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAGACTTCTTGTCCCCCGTGGTAAgg > 1:263101/1‑76 (MQ=255) gAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGa > 1:1645712/1‑76 (MQ=255) gAAATAAGTGTGGATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGa > 2:1217355/1‑76 (MQ=255) aaGTGTGGATACAGTATTATGGATCAAAACCCGTAGTCACAGCTGAAACTTCTTGTCACCCNTGGTAAGGAGCAAg > 1:1752996/1‑76 (MQ=255) aGTGTGGATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAgg > 1:567982/1‑76 (MQ=255) gtgGATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTa < 1:1557803/76‑1 (MQ=255) tgGATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTAt > 2:1354358/1‑76 (MQ=255) tgGATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTAt < 2:369274/76‑1 (MQ=255) ggATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATc > 2:1346175/1‑76 (MQ=255) ggATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATc > 1:1397413/1‑76 (MQ=255) ggATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATc > 1:1531760/1‑76 (MQ=255) ggATACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATc > 1:1815564/1‑76 (MQ=255) aTACCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTg < 1:1341088/76‑1 (MQ=255) tACCGTCTCATGGATCAAAACCCGCAGCTACTTCTGAGACTTTTTGTCACCCGTGGTAAGGAGGAAGGCTATCTGa < 1:1972911/76‑1 (MQ=255) aCCGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGAc > 1:1064064/1‑76 (MQ=255) cGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCt < 2:1048428/76‑1 (MQ=255) cGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCt > 1:1557746/1‑76 (MQ=255) cGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCt < 1:1471854/76‑1 (MQ=255) cGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCt < 2:567932/76‑1 (MQ=255) cGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCt < 2:588096/76‑1 (MQ=255) cGTCTTATGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCt < 2:719404/76‑1 (MQ=255) tCTTATCGAACAAAACCGGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAANGCNATCTGACCTAt > 2:310401/1‑76 (MQ=255) cTTATGGATCAAATCCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCTATg < 1:1346502/76‑1 (MQ=255) aTGGATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCTATGCCg < 2:1954993/76‑1 (MQ=255) ggATCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCTATGCCGAg < 2:1306871/76‑1 (MQ=255) | AACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCTATGCCGAG > REL606/3145797‑3145941 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |