| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 474,360 | Δ1 bp | coding (946/1677 nt) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 474,359 | 0 | T | . | 97.6% | 190.5 / ‑1.5 | 42 | coding (947/1677 nt) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (17/24); total (18/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01 | |||||||||||
GCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAAC > REL606/474286‑474430 | gCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACtac < 2:1312782/76‑3 (MQ=255) gATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGaa < 2:1366555/76‑1 (MQ=255) aTGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGaat > 2:785604/1‑76 (MQ=255) aTGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGaat < 1:351702/76‑1 (MQ=255) gTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGaataat > 1:888498/1‑76 (MQ=255) gTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGaataat < 1:302552/76‑1 (MQ=255) gTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGaataat > 1:701126/1‑76 (MQ=255) gTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGaataat > 1:6712/1‑76 (MQ=255) tAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCg > 1:1280555/1‑76 (MQ=255) tAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCg < 1:1314161/76‑1 (MQ=255) tAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCg > 2:596517/1‑76 (MQ=255) tGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCa < 2:487285/76‑1 (MQ=255) tGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCa < 2:378713/76‑1 (MQ=255) cGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCg > 1:914909/1‑76 (MQ=255) aCGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCg > 1:734082/1‑76 (MQ=255) aGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACt < 2:892013/76‑1 (MQ=255) aGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACt < 2:751573/76‑1 (MQ=255) ggccAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGcc < 2:36316/74‑1 (MQ=255) gACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGcc < 2:1516351/76‑1 (MQ=255) gACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGcc > 2:1478226/1‑76 (MQ=255) cAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGc < 2:431901/76‑1 (MQ=255) aaCAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCgg > 2:1522944/1‑76 (MQ=255) aCAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGt > 1:1354613/1‑76 (MQ=255) cAGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGtt > 1:1150206/1‑76 (MQ=255) aGTCGCACCAGTAAAAATGCGTCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTg < 2:591816/76‑1 (MQ=255) aGTCGCACCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTg < 2:1299199/76‑1 (MQ=255) cACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGa > 1:1314501/1‑76 (MQ=255) aCCAGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAAt < 1:1579420/76‑1 (MQ=255) agaaaaaaTGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAg < 1:657160/73‑1 (MQ=255) aGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAg < 2:1365936/76‑1 (MQ=255) aGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAg > 2:128006/1‑76 (MQ=255) aGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAg < 1:1421114/76‑1 (MQ=255) aGGAAAAATGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAg > 1:478845/1‑76 (MQ=255) aaaaaTGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAAt < 2:844706/76‑1 (MQ=255) aaaaaTGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAAt < 1:1507537/76‑1 (MQ=255) tGCGGCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACg > 2:439607/1‑76 (MQ=255) cgtcTAACGGC‑TACCAAACAGAATAAGCGCCAGCGTCGCCAGCGCTGCCAGCGGTTGCTGAATCAGAATTAACGGa < 1:1368163/73‑1 (MQ=255) ggCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGAt < 1:1157292/76‑1 (MQ=255) ggCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGAt > 2:226032/1‑76 (MQ=255) ggCTAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGAt < 1:641601/76‑1 (MQ=255) cTAACGGC‑TACCAAACAGAAAAATCGCCAGCGGCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCa < 2:1232042/76‑1 (MQ=255) tAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCaa < 1:921268/76‑1 (MQ=255) tAACGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCaa > 1:1459622/1‑76 (MQ=255) tAACGAC‑TACCAAACAGAAAAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCaa < 2:770382/76‑1 (MQ=255) aCGAC‑TACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAaac < 1:724010/76‑1 (MQ=255) | GCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAAC > REL606/474286‑474430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |