| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 795,321 | (G)6→5 | intergenic (+26/+16) | ECB_00736 → / ← ECB_00737 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 795,316 | 0 | G | . | 97.9% | 224.5 / ‑0.6 | 47 | intergenic (+21/+21) | ECB_00736/ECB_00737 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (19/27); total (19/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01 | |||||||||||
TTTGTCCTTAATTCCAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTAGGGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATCGTTCTTCTCCAGAAAGTGATA > REL606/795241‑795387 | tttGTCCTTAATTCCAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTAg < 2:1443621/76‑1 (MQ=255) ttGTCCTTAATTCCAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTAgg > 1:831824/1‑76 (MQ=255) tGTCCTTAATTCCAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTAggg > 1:1131942/1‑76 (MQ=255) tGTCCTTAATTCCAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTAggg > 1:1329602/1‑76 (MQ=255) gTCCTTAATTCCAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTAgggg > 1:304837/1‑76 (MQ=255) gTCCTTAATTCCAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTAgggg > 2:1338417/1‑76 (MQ=255) cTTAATTCCAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTAgggggta > 2:719356/1‑74 (MQ=255) cTTAATTCCAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTAgggggta > 2:158611/1‑74 (MQ=255) ttAATTCCAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTAgggggtac > 2:1473266/1‑73 (MQ=255) aTTCCAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTAcccc > 2:716169/1‑76 (MQ=255) ttCCAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTAccccc > 2:436732/1‑76 (MQ=255) ttCCAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTAccccc < 1:699196/76‑1 (MQ=255) ccAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCt > 1:1561326/1‑76 (MQ=255) ccAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCt < 2:1525180/76‑1 (MQ=255) ccAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCt < 2:1525195/76‑1 (MQ=255) cAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCta < 2:346412/76‑1 (MQ=255) gAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCtaat < 2:1305335/76‑1 (MQ=255) gAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCtaat < 2:473986/76‑1 (MQ=255) aaaTAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCtaata < 2:1165926/76‑1 (MQ=255) aaTAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCtaataa < 1:807272/76‑1 (MQ=255) aaTAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCtaataa < 2:1542753/76‑1 (MQ=255) aaTAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCtaataa < 1:630158/76‑1 (MQ=255) aaTAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCtaataa < 1:160354/76‑1 (MQ=255) aTAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTAGGGGGGTACCCCCCtaataa < 1:134667/76‑1 (MQ=255) aTAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATaaa < 2:1146416/76‑1 (MQ=255) tAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAAt < 2:331411/76‑1 (MQ=255) aaCCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATc < 2:1494070/76‑1 (MQ=255) aaCCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATc > 2:906969/1‑76 (MQ=255) cTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATCAgg < 2:401162/76‑1 (MQ=255) cTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATCAgg < 2:1157653/76‑1 (MQ=255) tGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTCTTATTA‑GGGGGTACCCCCCTAATAAATCAGGa > 2:338744/1‑76 (MQ=255) tGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGa > 2:803595/1‑76 (MQ=255) tGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGa < 2:744875/76‑1 (MQ=255) ggCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGAt > 2:825389/1‑76 (MQ=255) ggCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGAt > 2:727455/1‑76 (MQ=255) gCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGATg < 2:432156/76‑1 (MQ=255) aGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGATGTaa < 1:613840/76‑1 (MQ=255) aaaGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGATGTAatt < 2:731300/76‑1 (MQ=255) agaTAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTg < 2:1290011/76‑1 (MQ=255) agaTAATACGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTg < 1:399753/76‑1 (MQ=255) ataataCGTTGAAATTATGTGGTCTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGAt > 2:344488/1‑76 (MQ=255) cGTTGAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATc > 2:1161576/1‑76 (MQ=255) gAAATTATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATCGttc > 1:694192/1‑76 (MQ=255) tATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATCGTTCTtctc > 1:895696/1‑76 (MQ=255) tATGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATCGTTCTtctc > 2:191670/1‑76 (MQ=255) aTGTGGTGTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATCGTTCTTCTcc < 2:272138/76‑1 (MQ=255) gtggtgTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATCGTTCTTCTCCAg < 2:268311/76‑1 (MQ=255) gtggtgTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATCGTTCTTCTCCAg < 2:268326/76‑1 (MQ=255) gtggtgTATTATTA‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATCGTTCTTCTCCAg < 1:508653/76‑1 (MQ=255) tattatTA‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATCGTTCTTCTCCAGAAAGTg > 2:134539/1‑76 (MQ=255) tattatTA‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATCGTTCTTCTCCAGAAAGTg > 2:603376/1‑76 (MQ=255) tattatTA‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATCGTTCTTCTCCAGAAAGTg > 2:134524/1‑76 (MQ=255) attattA‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATCGTTCTTCTCCAGAAAGTga > 1:598058/1‑76 (MQ=255) tatta‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATCGTTCTTCTCCAGAAAGTgata > 2:504067/1‑76 (MQ=255) tatta‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATCGTTCTTCTCCAGAAAGTgata > 2:1428183/1‑76 (MQ=255) tatta‑GGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATCGTTCTTCTCCAGAAAGTgata < 1:1231526/76‑1 (MQ=255) | TTTGTCCTTAATTCCAGAAATAACCTGGCCAGAAAGGCCAGAGATAATACGTTGAAATTATGTGGTGTATTATTAGGGGGGTACCCCCCTAATAAATCAGGATGTAATTATTTTTCTGATTTTATCGTTCTTCTCCAGAAAGTGATA > REL606/795241‑795387 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |