| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | REL606 | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | = 1733287 | 0 (0.000) | 37 (0.850) +AACGTATG |
28/114 | 0.2 | 100% | coding (323/1413 nt) | pykF | pyruvate kinase |
| ? | REL606 | 1733288 = | 0 (0.000) | coding (324/1413 nt) | pykF | pyruvate kinase | |||||
CTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > REL606/1733215‑1733287‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTAT > REL606/1733288‑1733359 |||||||| CTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAAC < 2:208109/76‑1 TGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTA < 1:1399800/76‑1 TGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTA < 1:295268/76‑1 GAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTAT > 2:592426/1‑76 AAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATG > 2:593530/1‑76 AAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGA < 2:571911/76‑1 GGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTA < 1:1122350/76‑1 GTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTAT < 1:1426326/76‑1 TCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATG > 2:383691/1‑76 TCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATG > 2:190281/1‑76 CAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGA < 2:616196/76‑1 CAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGA > 2:524919/1‑76 TTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTT < 2:908895/76‑1 TTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTC > 1:1566532/1‑76 TACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCA < 1:795361/76‑1 ACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAAAGTATGAACGTATGAAGGTTTCAC > 1:1209135/1‑76 TTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTAC > 2:1376866/1‑76 TCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACT < 1:1380320/76‑1 TCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACT > 2:268785/1‑76 CACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTG < 2:1538635/76‑1 CACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTG > 1:881256/1‑76 ACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGA < 1:1246452/76‑1 ACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGA < 1:108442/76‑1 CTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTCTCACTACTGACCTG > 1:1529847/1‑76 GATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTC > 1:1424641/1‑76 GATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTC < 1:524100/76‑1 GATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTC > 1:457563/1‑76 ATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCT < 2:1193305/76‑1 ATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTG > 1:1537680/1‑76 CTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGC > 1:108212/1‑76 CTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGC > 1:407852/1‑76 TGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCA < 1:310862/76‑1 TGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCA > 1:507414/1‑76 TGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCA < 2:108002/76‑1 TGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCA > 1:967227/1‑76 GTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAA > 1:505032/1‑76 CAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGGCCTGTCTGTTGGCAACACCGTACTGG < 1:605907/76‑1 GGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATG < 2:21326/76‑1 GGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATG < 2:470942/76‑1 GCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCT < 2:751767/76‑1 GGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGA < 1:778218/76‑1 AACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCG > 2:701277/1‑76 CGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGT > 2:1386321/1‑76 GTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTA > 2:1408073/1‑76 GTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTA > 1:13699/1‑76 TATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTAT > 1:438768/1‑76 |||||||| CTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > REL606/1733215‑1733287‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTAT > REL606/1733288‑1733359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |