| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,492,082 | 0 | T | G | 37.5% | 99.3 / 19.0 | 56 | V490G (GTG→GGG) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (4/31); new base G (21/0); total (25/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.39e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTGACC > REL606/3492009‑3492157 | gCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggggg > 1:624444/1‑76 (MQ=255)gCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggggg > 2:745129/1‑76 (MQ=255)gCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggggg > 1:809996/1‑76 (MQ=255) cTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtgggggggg > 1:1439485/1‑73 (MQ=255) cTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtgggggggg > 1:1403769/1‑73 (MQ=255) cTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGt < 1:771598/76‑1 (MQ=255) cTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGt > 1:1382260/1‑76 (MQ=255) gtgGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGtat < 2:914976/76‑1 (MQ=255) gtgGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGtat < 1:255661/76‑1 (MQ=255) tgGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGgata > 1:114723/1‑76 (MQ=255) gCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACg < 1:811084/76‑1 (MQ=255) cGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgc < 2:817700/76‑1 (MQ=255) cGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACgc > 1:103492/1‑76 (MQ=255) tCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTAGACgcag > 1:645214/1‑76 (MQ=255) cGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgcagc < 2:966333/76‑1 (MQ=255) cGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgcagc < 2:1038556/76‑1 (MQ=255) cGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACggagc > 2:1409846/1‑76 (MQ=255) cGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACgcagc > 1:501269/1‑76 (MQ=255) gCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgcagca < 2:241866/76‑1 (MQ=255) acaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACGCAGCAGa > 1:1317872/1‑76 (MQ=255) acaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACGCAGCAGa > 1:414750/1‑76 (MQ=255) caAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGaa < 1:683964/76‑1 (MQ=255) caAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATAAGCAGCAGaa > 2:1406218/1‑76 (MQ=255) aaaaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAg < 2:639047/76‑1 (MQ=255) aaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCt < 2:194089/76‑1 (MQ=255) cAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGAAGCTGt > 2:1010757/1‑76 (MQ=255) cATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTg < 2:1088228/76‑1 (MQ=255) cATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTg < 2:706975/76‑1 (MQ=255) aTGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTgg > 1:1543166/1‑76 (MQ=255) tgtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGc < 1:1205934/76‑1 (MQ=255) gtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGcg < 1:337196/76‑1 (MQ=255) gtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGcg < 1:473574/76‑1 (MQ=255) gtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGcg < 1:939450/76‑1 (MQ=255) gtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGcg < 1:512218/76‑1 (MQ=255) gtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGcg < 1:485155/76‑1 (MQ=255) gCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGgcggcg > 2:121429/1‑76 (MQ=255) gCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACGCAGCAGAAGCTGTCGCTGgcggcg > 2:122043/1‑76 (MQ=255) gCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGAAGCTGTCGCTGgcggcg > 2:1183048/1‑76 (MQ=255) gCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGGCGCTGgcggcg > 2:1490059/1‑76 (MQ=255) aaaTGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAACCGTCGCTGGCGCCGTgt > 1:1193061/1‑75 (MQ=255) gCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGGGTATACGCAGCAGAAGCTGTCGCTGGCGGCGTGGTTTa > 1:675575/1‑76 (MQ=255) cGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTaa < 2:967186/76‑1 (MQ=255) aTCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGGGGTTTTATGt > 1:456038/1‑76 (MQ=255) aTCAACAATCTCGTGTGAGTCAGTGGCTGGCGGAGTATAAGCAGCACAAGCTGTCGCCGGCGCCGGGATTTAAtct > 1:527088/1‑74 (MQ=255) tCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTa < 2:511781/76‑1 (MQ=255) caacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTaa < 2:690654/76‑1 (MQ=255) acaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAgg < 2:419462/76‑1 (MQ=255) acaaTCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGGCGTGGTTTTATGTAAgg > 2:393750/1‑76 (MQ=255) caaTCTCGTGTGAGTCAGTGGCTGGGGGGGTATACGCAGCAGAAGCTGTCGCTGGGGGCGTGATTTAATGTAAggg > 2:365903/1‑75 (MQ=255) caaTCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGGTTTTATGTAAggg > 2:1573657/1‑75 (MQ=255) caaTCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGGCGTGATTTAATGTAAggg > 2:916554/1‑75 (MQ=255) aaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTg < 1:1268708/76‑1 (MQ=255) aaTCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAgggg > 2:540371/1‑74 (MQ=255) tctcGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGc < 1:476533/76‑1 (MQ=255) tctcGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGc < 2:123217/76‑1 (MQ=255) cGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAg > 2:1181652/1‑76 (MQ=255) gtgagtgaGTGGCTGGTGGAGTATACGCAGTAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTc < 1:684780/76‑1 (MQ=255) gtgAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTc < 1:1517020/76‑1 (MQ=255) gtgAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTc < 1:129562/76‑1 (MQ=255) tCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGAtt < 1:48151/76‑1 (MQ=255) cAGTGGCTGGTGGCGTATACGCAGCAGAAGCTTTCGCTGGCGTCGGGGTTTAATGTAAGGTGGGCCGGGCAGAttt > 2:1001717/1‑76 (MQ=255) cAGTGGCTGGGGGGGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGGTTTTATGTAAGGTGGGCAGGGCAGAttt > 2:330073/1‑76 (MQ=255) cAGTGGCTGGGGGGGGATACGCAGCAGAAGCTGTCGCTGGCGGCGGGGTTTTATGTAAGGGGGCCAGGGCAGAttt > 2:1332760/1‑76 (MQ=255) tGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCaa < 1:1502008/76‑1 (MQ=255) gCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAAta < 1:852990/76‑2 (MQ=255) cTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGGCAGATTTTAATCt > 2:1066020/1‑76 (MQ=255) tggAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTGAcc > 2:438352/1‑76 (MQ=255) tggAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTGAcc < 2:1056371/76‑1 (MQ=255) | GCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTGACC > REL606/3492009‑3492157 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |