| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,451,762 | T→A | I144F (ATC→TTC) | acpD ← | acyl carrier protein phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,451,762 | 0 | T | A | 97.9% | 166.2 / NA | 48 | I144F (ATC→TTC) | acpD | acyl carrier protein phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (15/32); minor base C (0/1); total (15/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAG > REL606/1451689‑1451832 | tCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAAcc < 1:1278012/76‑1 (MQ=255) cGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAAccc > 1:258965/1‑76 (MQ=255) cGGTAATGCCGATAAAGCCGAGGAACGTGGACAGANACGGCGTCACCAGGTCCGTTGGNCCATCTTTGTGGAAccc > 1:798665/1‑76 (MQ=255) ggTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccg < 2:1129782/76‑1 (MQ=255) gTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccgc > 2:262146/1‑76 (MQ=255) tAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccgcc < 2:865519/76‑1 (MQ=255) tAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccgcc > 1:797888/1‑76 (MQ=255) tAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccgcc > 1:708778/1‑76 (MQ=255) tAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccgcc < 1:430547/76‑1 (MQ=255) aaTGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccgccg < 1:1015548/76‑1 (MQ=255) aaTGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccgccg < 2:1028007/76‑1 (MQ=255) aaTGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccgccg < 1:913178/76‑1 (MQ=255) tGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCcgcg < 1:1537269/76‑1 (MQ=255) tGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCcgcg < 1:88214/76‑1 (MQ=255) cGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCt < 2:1557575/76‑1 (MQ=255) gaaaGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGt < 2:778435/75‑1 (MQ=255) aaaGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGta < 2:343234/76‑1 (MQ=255) aaaGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGta < 2:169479/76‑1 (MQ=255) aaaGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGta < 2:1252209/76‑1 (MQ=255) aaaGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGta < 1:217222/76‑1 (MQ=255) aGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGtaat < 1:1184317/76‑1 (MQ=255) gCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGtaata < 1:2811/76‑1 (MQ=255) gAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATg < 1:426178/76‑1 (MQ=255) aCAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCtttttt < 2:1356460/76‑1 (MQ=255) aCAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCtttttt < 1:253838/76‑1 (MQ=255) aCAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCtttttt < 2:1332198/76‑1 (MQ=255) aCAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCtttttt > 1:592485/1‑76 (MQ=255) tACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCg < 2:436012/76‑1 (MQ=255) aCGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGt > 2:221513/1‑76 (MQ=255) aCGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTAACCGt > 2:392297/1‑76 (MQ=255) cGGCGTCCCCAGGTCCGTTGGTCCTTCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGtt < 1:418806/76‑1 (MQ=255) cGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGtt > 1:56948/1‑76 (MQ=255) cGTCCCCAGGTCCGTTGGTCCATCTTTGTGGACCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTAcc < 1:723883/76‑1 (MQ=255) cGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTAcc < 2:520165/76‑1 (MQ=255) cGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTAcc < 1:418795/76‑1 (MQ=255) cGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTAcc < 1:1010361/76‑1 (MQ=255) tCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAg > 1:937063/1‑76 (MQ=255) cACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGa < 1:465978/76‑1 (MQ=255) aCCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGAc > 1:347924/1‑76 (MQ=255) ccAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGAcc > 2:1369461/1‑76 (MQ=255) ntCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGACGGCTTTTTTACCCGTTACCAGACCATcc > 1:1579925/2‑76 (MQ=255) ccGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCgg < 1:44251/76‑1 (MQ=255) ccGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCgg > 2:245430/1‑76 (MQ=255) cGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGCTGGCTTTTTTACCCCTTACCAGACCTCCCGGa > 1:764110/1‑76 (MQ=255) tGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCg < 1:1320721/76‑1 (MQ=255) ggTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGt > 1:109484/1‑76 (MQ=255) ccATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTtct < 2:975276/76‑1 (MQ=255) cATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTtctc > 1:738465/1‑76 (MQ=255) aTCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCg < 1:921559/76‑1 (MQ=255) ttGTGGACCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGtat < 2:341421/76‑1 (MQ=255) tgtgGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGtata > 2:1088845/1‑76 (MQ=255) gtgGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAg < 1:697785/76‑1 (MQ=255) | TCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAG > REL606/1451689‑1451832 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |