Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,359,374	T→C	F346L (TTC→CTC)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,359,374	0	T	C	100.0%	174.8 / NA	42	F346L (TTC→CTC)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/16);  total (26/16)

TGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACAT  >  REL606/2359301‑2359444                                                                          |                                                                       tGCTTTCGCTCCCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTc                                                                      <  1:616718/76‑1 (MQ=255) tGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTc                                                                      <  1:1310245/76‑1 (MQ=255)  gCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCa                                                                     >  1:1047719/1‑76 (MQ=255)  gCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCa                                                                     <  2:1347402/76‑1 (MQ=255)    tttCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAAc                                                                   <  1:1012880/76‑1 (MQ=255)       cGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTg                                                                >  1:1312393/1‑76 (MQ=255)       cGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTg                                                                >  1:683322/1‑76 (MQ=255)         cTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGa                                                              >  2:1007895/1‑76 (MQ=255)         cTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGa                                                              >  1:798068/1‑76 (MQ=255)         cTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGa                                                              >  1:1520265/1‑76 (MQ=255)          tACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGaa                                                             >  2:732400/1‑76 (MQ=255)           aCCGGCCTGCCGGTATTTATGGTGAACACCAACACCTCGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAg                                                            >  2:52524/1‑76 (MQ=255)             cGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGtt                                                          <  2:1338019/76‑1 (MQ=255)               gCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTcc                                                        >  1:1428865/1‑76 (MQ=255)                   gCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGtt                                                    >  1:33186/1‑76 (MQ=255)                         aTTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGAt                                              >  1:431709/1‑76 (MQ=255)                           ttATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCa                                            >  1:1273659/1‑76 (MQ=255)                           ttATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCa                                            >  1:1362294/1‑76 (MQ=255)                             aTGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACg                                          >  1:745759/1‑76 (MQ=255)                               ggTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGaa                                        >  1:896728/1‑76 (MQ=255)                                gTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAAc                                       <  1:1562627/76‑1 (MQ=255)                                gTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAAc                                       <  2:292967/76‑1 (MQ=255)                                        caacaccTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCgag                               >  2:1167460/1‑76 (MQ=255)                                         aacaccTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCgaga                              <  1:1482665/76‑1 (MQ=255)                                         aacaccTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCgaga                              >  2:173047/1‑76 (MQ=255)                                             ccTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGt                          <  1:487339/76‑1 (MQ=255)                                              cTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGtt                         <  1:815526/76‑1 (MQ=255)                                              cTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGtt                         <  2:1375248/76‑1 (MQ=255)                                               tGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTc                        >  2:92279/1‑76 (MQ=255)                                                   aGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGa                    >  2:194689/1‑76 (MQ=255)                                                    gACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGaa                   <  1:1081712/76‑1 (MQ=255)                                                         ctctGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGt              >  1:1116720/1‑76 (MQ=255)                                                           ctGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTg            >  1:1473558/1‑76 (MQ=255)                                                           ctGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTg            >  1:104822/1‑76 (MQ=255)                                                            tGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGc           <  2:44012/76‑1 (MQ=255)                                                             gAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt          >  2:1372620/1‑76 (MQ=255)                                                               gCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTaa        <  1:12472/76‑1 (MQ=255)                                                                ccTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAAc       >  1:75001/1‑76 (MQ=255)                                                                 cTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGCTCAGGAATACGTTGCTAACt      >  1:1357576/1‑76 (MQ=255)                                                                     agagCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACAt  <  2:126047/76‑1 (MQ=255)                                                                     agagCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACAt  >  2:442619/1‑76 (MQ=255)                                                                     agagCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACAt  <  2:828631/76‑1 (MQ=255)                                                                          |                                                                       TGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACAT  >  REL606/2359301‑2359444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑