Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,875,632	(T)7→8	intergenic (‑66/+287)	glmU ← / ← atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,875,625	1	.	T	100.0%	147.6 / NA	38	intergenic (‑59/+294)	glmU/atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (15/23);  total (15/23)

ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGC  >  REL606/3875550‑3875692                                                                             |                                                                    aTAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACa                                                                      <  2:578705/76‑1 (MQ=255) aTAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACa                                                                      <  2:1837233/76‑1 (MQ=255)   aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACTAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt                                                                   <  2:1635615/76‑1 (MQ=255)     cATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttt                                                                 <  1:1743986/76‑1 (MQ=255)       ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttttt                                                               >  2:442827/1‑76 (MQ=255)         attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttttttt                                                             <  2:103999/76‑2 (MQ=255)          ttCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttttttttc                                                            <  1:1156312/76‑3 (MQ=255)               cATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGt                                                        >  1:1748446/1‑76 (MQ=255)                aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTg                                                       <  1:39379/76‑1 (MQ=255)                aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTg                                                       <  1:1543465/76‑1 (MQ=255)                  aCGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTAAAAAAACTACATTTTTTTTCATCGTGaa                                                     >  1:1262534/1‑76 (MQ=255)                     cgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATg                                                  >  2:1684384/1‑76 (MQ=255)                      gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATgg                                                 >  1:1434052/1‑76 (MQ=255)                        ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGAc                                               <  1:1260599/76‑1 (MQ=255)                          tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACag                                             <  1:619434/76‑1 (MQ=255)                           gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACaga                                            <  2:1725804/76‑1 (MQ=255)                           gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACaga                                            <  2:1941017/76‑1 (MQ=255)                           gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACaga                                            <  2:556968/76‑1 (MQ=255)                           gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACaga                                            <  1:708117/76‑1 (MQ=255)                               gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGAt                                        <  2:1778376/76‑1 (MQ=255)                                tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATa                                       <  2:683355/76‑1 (MQ=255)                                   tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAAt                                    <  2:1056227/76‑1 (MQ=255)                                   tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAAt                                    <  2:628807/76‑1 (MQ=255)                                    ntGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAAtt                                   >  1:1516462/2‑76 (MQ=255)                                     tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTg                                  >  1:1689890/1‑76 (MQ=255)                                       agaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGtt                                >  1:2018409/1‑76 (MQ=255)                                         aaCGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCa                              <  1:355253/76‑1 (MQ=255)                                          aCGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCaa                             <  2:661140/76‑1 (MQ=255)                                          aCGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCaa                             <  2:1469934/76‑1 (MQ=255)                                           cGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt                            <  1:1385211/76‑1 (MQ=255)                                             aaTTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa                          <  1:684088/76‑1 (MQ=255)                                              aTTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAAc                         <  1:946280/76‑1 (MQ=255)                                              aTTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAAc                         <  1:1296122/76‑1 (MQ=255)                                                 tAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa                      >  1:492443/1‑76 (MQ=255)                                                 tAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa                      >  2:103279/1‑76 (MQ=255)                                                  aaaCCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt                     >  2:2024755/1‑76 (MQ=255)                                                    aCCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTa                   >  1:1398620/1‑76 (MQ=255)                                                      cGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATc                 >  1:1407127/1‑76 (MQ=255)                                                         ttCTCCTTGAAAAAACTACATTTTTTTTCATCGTGAAATTGACAGAGGATAAATTGTTCAATAACGGATTATcccc              <  2:1766786/76‑1 (MQ=255)                                                          tCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg             >  1:1374980/1‑76 (MQ=255)                                                             ccTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg          <  2:1956084/76‑1 (MQ=255)                                                              cTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa         >  1:1663155/1‑76 (MQ=255)                                                                tGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg       <  1:1846715/76‑1 (MQ=255)                                                                   aaaaaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa    <  2:1308949/76‑1 (MQ=255)                                                                    aaaaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg   <  2:669906/76‑1 (MQ=255)                                                                     aaaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgc  >  2:420452/1‑76 (MQ=255)                                                                             |                                                                    ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGC  >  REL606/3875550‑3875692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑