Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,795,6500AC29.5% 99.2 / 10.9 44V39G (GTG→GGG) celFcryptic phospho‑beta‑glucosidase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (27/4);  new base C (0/13);  total (27/17)
Fisher's exact test for biased strand distribution p-value = 4.58e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGC  >  REL606/1795575‑1795722
                                                                           |                                                                        
aCGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATcca                                                                          >  2:1381638/1‑76 (MQ=255)
  gCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATccacc                                                                        >  1:86486/1‑76 (MQ=255)
  gCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATccacc                                                                        >  1:666673/1‑76 (MQ=255)
    cAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAg                                                                      >  1:1034973/1‑76 (MQ=255)
     aGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGc                                                                     <  1:1012170/76‑1 (MQ=255)
       cGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCa                                                                   <  2:1310720/76‑1 (MQ=255)
       cGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCa                                                                   <  1:1523780/76‑1 (MQ=255)
         ttATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATa                                                                 >  2:988071/1‑76 (MQ=255)
         ttATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATa                                                                 >  2:111587/1‑76 (MQ=255)
          tATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCCCCAGCCATaa                                                                <  2:375827/76‑1 (MQ=255)
          tATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATaa                                                                >  1:404638/1‑76 (MQ=255)
                     cGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCg                                                     >  1:1043236/1‑76 (MQ=255)
                      gTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCCCCTTCGACATCCCCCAGCCATAATTCGCTGACCgg                                                    <  1:1622031/76‑1 (MQ=255)
                       ttGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGc                                                   >  1:1431525/1‑76 (MQ=255)
                        tGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTCCCCCCTTCGACACCCACCAGCCATAATTCGCTGACCGGCa                                                  <  1:1172694/76‑1 (MQ=255)
                        tGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCa                                                  >  2:628391/1‑76 (MQ=255)
                           cAGAGATCGAAAATAATATCCAGTTTCGCTTTCCCCCCTTCGACACCCCCCAGCCATAATTCGCTGACCGGCAAtt                                               <  2:1009458/76‑1 (MQ=255)
                                 tCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGAt                                         >  2:972906/1‑76 (MQ=255)
                                  cGAAAATAATATCCAGTTTCGCTTTCCCCCCTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATa                                        <  2:80752/76‑1 (MQ=255)
                                    aaaaTAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAAc                                      >  2:1985687/1‑76 (MQ=255)
                                     aaaTAATCTCCAGTTTCGTTTTACCCCCTCCGACACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACg                                     <  2:188759/76‑1 (MQ=255)
                                     aaaTAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACg                                     >  1:1120315/1‑76 (MQ=255)
                                        taataTCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCtt                                  >  2:1037047/1‑76 (MQ=255)
                                         aataTCCAGTTTCGCTTTACCCCCTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTta                                 <  1:2016639/76‑1 (MQ=255)
                                          atatCCAGTTTCGCTTTACCCCCTTCGACACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaa                                <  1:1738732/76‑1 (MQ=255)
                                          atatCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaa                                >  2:1075365/1‑76 (MQ=255)
                                            atCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaata                              >  1:1484723/1‑76 (MQ=255)
                                            atCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaata                              >  1:1293359/1‑76 (MQ=255)
                                              ccAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATaaa                            >  1:1264135/1‑76 (MQ=255)
                                                 gTTTCGCTTTACCCCCTCCGACACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATca                         <  2:199309/76‑2 (MQ=255)
                                                  tttCGCTTTCCCCCCTCCGACACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCt                        <  1:987160/76‑1 (MQ=255)
                                                   ttCGCTTTCCCCCCTTCGACACCCCCCAGCCATAATTTGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCtt                       <  1:793424/76‑1 (MQ=255)
                                                   ttCGCTTTACCCCCTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCtt                       <  1:988071/76‑1 (MQ=255)
                                                   ttCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCtt                       >  2:1444268/1‑76 (MQ=255)
                                                   ttCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCtt                       >  1:167738/1‑76 (MQ=255)
                                                    tCGCTTTCCCCCCTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTc                      <  1:1313328/76‑1 (MQ=255)
                                                     cGCTTTACCCCCTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTcc                     <  1:1978469/76‑1 (MQ=255)
                                                     cGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTcc                     >  2:39776/1‑76 (MQ=255)
                                                       cTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAg                   >  1:1506200/1‑76 (MQ=255)
                                                        tttACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGt                  >  1:93302/1‑76 (MQ=255)
                                                        tttACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGt                  >  1:23973/1‑76 (MQ=255)
                                                        tttACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGt                  >  2:735082/1‑76 (MQ=255)
                                                              accTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTcc            >  1:42982/1‑76 (MQ=255)
                                                               ccTTCGACATCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCg           <  1:206808/76‑1 (MQ=255)
                                                               ccTCCGCCACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCg           <  1:179055/76‑1 (MQ=255)
                                                                   ngACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGt       >  1:213738/2‑76 (MQ=255)
                                                                    gACACCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGta      <  1:1829301/76‑1 (MQ=255)
                                                                     aCATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGtat     >  2:1411872/1‑76 (MQ=255)
                                                                       aTCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAg   >  1:1242002/1‑76 (MQ=255)
                                                                        tCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAgc  >  1:1271177/1‑76 (MQ=255)
                                                                           |                                                                        
ACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGC  >  REL606/1795575‑1795722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑