Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,875,632	(T)7→8	intergenic (‑66/+287)	glmU ← / ← atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,875,625	1	.	T	100.0%	130.6 / NA	33	intergenic (‑59/+294)	glmU/atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (14/19);  total (14/19)

ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAG  >  REL606/3875550‑3875691                                                                             |                                                                   aTAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACa                                                                     >  2:231525/1‑76 (MQ=255)         attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttttttt                                                            >  2:542769/1‑75 (MQ=255)          ttCAAGATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttttttttc                                                           <  2:844974/76‑3 (MQ=255)                   cgcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGaaa                                                   <  2:762644/76‑1 (MQ=255)                   cgcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGaaa                                                   <  2:70046/76‑1 (MQ=255)                    gcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAAt                                                  <  1:1541727/76‑1 (MQ=255)                        ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGAc                                              >  2:1399506/1‑76 (MQ=255)                         cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACa                                             <  2:70507/76‑1 (MQ=255)                         cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACa                                             >  1:617467/1‑76 (MQ=255)                         cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACa                                             >  2:458057/1‑76 (MQ=255)                          tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACag                                            >  1:903548/1‑76 (MQ=255)                           gACTGTAATTTGGGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACaga                                           <  2:418928/76‑1 (MQ=255)                           gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACaga                                           <  2:904361/76‑1 (MQ=255)                            aCTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACagag                                          <  2:133792/76‑1 (MQ=255)                               gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGAt                                       <  1:187424/76‑1 (MQ=255)                               gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGAt                                       >  1:412083/1‑76 (MQ=255)                                 aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATan                                     <  1:1285860/76‑2 (MQ=255)                                 aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATaa                                     >  2:848406/1‑76 (MQ=255)                                 aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATaa                                     >  1:1102703/1‑76 (MQ=255)                                 aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATaa                                     >  2:231589/1‑76 (MQ=255)                                  aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATaaa                                    >  2:562682/1‑76 (MQ=255)                                        gaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTc                              <  1:1499603/76‑1 (MQ=255)                                         aaCGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCa                             >  1:576215/1‑76 (MQ=255)                                         aaCGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCa                             >  2:652255/1‑76 (MQ=255)                                           cGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt                           <  1:340445/76‑1 (MQ=255)                                            gAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATa                          <  1:882423/76‑1 (MQ=255)                                              aTTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAAc                        >  2:937263/1‑76 (MQ=255)                                               tttAAACCCCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg                       <  2:1230222/76‑1 (MQ=255)                                                 tAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTTAATAACGGa                     >  1:1047770/1‑76 (MQ=255)                                                   aaCCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt                   <  1:762672/76‑1 (MQ=255)                                                    aCCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTa                  <  2:978882/76‑1 (MQ=255)                                                     ccGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt                 <  1:49033/76‑1 (MQ=255)                                                           cACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGc           >  2:583514/1‑76 (MQ=255)                                                             ccTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg         <  2:778742/76‑1 (MQ=255)                                                                   aaaaaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa   <  2:197919/76‑1 (MQ=255)                                                                    aaaaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg  <  1:405265/76‑1 (MQ=255)                                                                             |                                                                   ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAG  >  REL606/3875550‑3875691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑