| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 318,568 | T→C | Q140Q (CAA→CAG) | yahN ← | neutral amino‑acid efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 318,568 | 0 | T | C | 97.5% | 159.4 / ‑3.7 | 40 | Q140Q (CAA→CAG) | yahN | neutral amino‑acid efflux system |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (15/24); total (16/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
CCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGGCGCTAATCGGTTGTTGT > REL606/318495‑318640 | ccATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTg < 2:39274/76‑1 (MQ=255) aTTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCg > 2:137956/1‑76 (MQ=255) aaCGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGtt < 2:1028512/76‑1 (MQ=255) cccATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTagagag < 2:489678/76‑1 (MQ=255) ccATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAgagaga > 2:86341/1‑76 (MQ=255) ccATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAgagaga < 2:1123636/76‑1 (MQ=255) cATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGAt > 2:1341677/1‑76 (MQ=255) tGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGta < 1:124572/76‑1 (MQ=255) tGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGta < 2:1583575/76‑1 (MQ=255) ggTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGtaa < 2:1477863/76‑1 (MQ=255) ggTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGtaa > 2:654785/1‑76 (MQ=255) ggTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGtaa < 1:674923/76‑1 (MQ=255) ggTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGtaa < 2:250305/76‑1 (MQ=255) gtgtTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGtaat < 2:29783/76‑1 (MQ=255) gtgtTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGtaat > 1:1193120/1‑76 (MQ=255) tgtTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGtaatt < 1:83873/76‑1 (MQ=255) tgtTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGtaatt > 1:1245873/1‑76 (MQ=255) gtTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGtaatta < 2:542992/76‑1 (MQ=255) tttCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGtaattaa < 2:168562/76‑1 (MQ=255) cGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATcc > 2:299388/1‑76 (MQ=255) cGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATcc < 2:1634380/76‑1 (MQ=255) ggCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATCcg < 1:308823/76‑1 (MQ=255) gCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATCcgc < 1:339107/76‑1 (MQ=255) aTTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATCCgcgg > 2:1144741/1‑76 (MQ=255) ttAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATCCgcggcg > 1:1184386/1‑76 (MQ=255) tAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGa < 2:1537650/76‑1 (MQ=255) aaTGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGaa > 1:554644/1‑76 (MQ=255) aTGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGaaa > 1:704921/1‑76 (MQ=255) tGTTACTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGaaaa > 1:1171712/1‑76 (MQ=255) tACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAg > 2:1440105/1‑76 (MQ=255) cTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGAc > 2:833144/1‑76 (MQ=255) cTGAGAAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGAc < 2:1659162/76‑1 (MQ=255) cTGAAAAAATACTGATAAAAAATAAAACGGTATGAGGGTTAGAGAGATCGGTAATTAATCTGCGGCGAAAAAAGaa > 1:481692/1‑75 (MQ=255) agaAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATa < 2:1376151/76‑1 (MQ=255) agaAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATa < 2:1172693/76‑1 (MQ=255) agaAAATACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATa < 2:619295/76‑1 (MQ=255) aaaaTACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACGTAcc < 1:823528/76‑1 (MQ=255) aaaTACTGATAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCa < 1:133178/76‑1 (MQ=255) aTAAAAAATAAAACGGTCTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGgcgc > 2:1110811/1‑76 (MQ=255) aaaaCGGTCTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGGCGCTAATCGgtt > 1:1239402/1‑76 (MQ=255) cGGTCTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGGCGCTAATCGGttgttg > 1:1339165/1‑76 (MQ=255) ggTCTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGGCGCTAATCGGTtgttgt < 2:1563081/76‑1 (MQ=255) | CCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTAATCCGCGGCGAAAAAAGACATACCAGGGGGCGCTAATCGGTTGTTGT > REL606/318495‑318640 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |