| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,511,637 | A→G | intergenic (+101/‑438) | acrD → / → yffB | aminoglycoside/multidrug efflux system/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,511,637 | 0 | A | G | 95.2% | 239.4 / 1.0 | 62 | intergenic (+101/‑438) | acrD/yffB | aminoglycoside/multidrug efflux system/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (3/0); new base G (37/22); total (40/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTAT > REL606/2511564‑2511709 | gtCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAAttgct > 1:473771/1‑76 (MQ=255) tCGCGTTGTTCAACTTTCCGATTAACAACCTGCTCAGCGGGTTCTTGCTGTTTGTCCTTTGTCTCAGGAAttgctt < 2:956992/76‑1 (MQ=255) tCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAAttgctt > 1:1626004/1‑76 (MQ=255) cGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAAttgctta < 1:1185922/76‑1 (MQ=255) cGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAAttactta > 1:621064/1‑76 (MQ=255) ccTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACg > 2:830815/1‑76 (MQ=255) tttttCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGaa < 2:1344522/76‑1 (MQ=255) ttttCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAg > 2:1184946/1‑76 (MQ=255) ttttCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAg > 2:1213641/1‑76 (MQ=255) tttCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGc < 1:656185/76‑1 (MQ=255) tttCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGc < 2:1611277/76‑1 (MQ=255) tCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCAt < 1:314335/76‑1 (MQ=255) tCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCAt > 2:998414/1‑76 (MQ=255) aaCTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATaa > 1:1210638/1‑76 (MQ=255) ccGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGa < 1:1543212/76‑1 (MQ=255) cGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGAt > 2:1114969/1‑76 (MQ=255) ttAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATaaa < 1:203657/76‑1 (MQ=255) ttAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATaaa > 1:206934/1‑76 (MQ=255) aaGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGt > 2:609854/1‑76 (MQ=255) aGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTc > 1:1024770/1‑76 (MQ=255) aCCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCttt < 1:211126/76‑1 (MQ=255) aCCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCttt > 1:258287/1‑76 (MQ=255) ccTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTc > 1:1060707/1‑76 (MQ=255) cTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTcc < 1:1506881/76‑1 (MQ=255) tGCTCAGCGGGTTCTTGCTGTTTGTACTATGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCa < 2:66716/76‑1 (MQ=255) cTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGt > 2:379132/1‑76 (MQ=255) tCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGtt > 2:808705/1‑76 (MQ=255) aGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTcc > 2:482397/1‑76 (MQ=255) aGCGGGTTCTTGCTGTTTGTACTTGGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTcc < 2:246727/76‑1 (MQ=255) cGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTcccc > 2:1615579/1‑76 (MQ=255) gggTTCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCa > 1:739179/1‑76 (MQ=255) ttCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGtt > 1:137482/1‑76 (MQ=255) ttCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGtt > 1:1394073/1‑76 (MQ=255) tCTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTc > 2:885562/1‑76 (MQ=255) cTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCa > 2:809556/1‑76 (MQ=255) cTTGCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCa > 2:809571/1‑76 (MQ=255) gCTGTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGt > 1:1141134/1‑76 (MQ=255) tGTTTGTACTTGGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTc > 1:289867/1‑76 (MQ=255) gTTTGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCa > 2:1205589/1‑76 (MQ=255) tttGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCaa > 1:1624840/1‑76 (MQ=255) ttGTACTTTGTCTCAGGAATTGCTTCCGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAAt > 2:1642994/1‑76 (MQ=255) ttGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAAt > 1:932220/1‑76 (MQ=255) ttGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAAt > 2:106349/1‑76 (MQ=255) tGTACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATc > 1:306572/1‑76 (MQ=255) tACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCAt > 1:638504/1‑76 (MQ=255) tACTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCAt > 1:1350716/1‑76 (MQ=255) aCTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATa > 1:1602057/1‑76 (MQ=255) aCTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATa > 2:1301236/1‑76 (MQ=255) cTTTGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaa < 1:1575759/76‑1 (MQ=255) tttGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaac < 1:83426/76‑1 (MQ=255) tttGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaac < 1:758409/76‑1 (MQ=255) tttGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaac > 1:760795/1‑76 (MQ=255) ttGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaaca < 1:1616942/76‑1 (MQ=255) tGTCTCAGGAATTGCTTGCGAAGCATGACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaacaa < 2:1183228/76‑1 (MQ=255) tGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaacaa > 1:998392/1‑76 (MQ=255) tGTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaacaa > 2:423809/1‑76 (MQ=255) gTCTCAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATaacaac > 2:626065/1‑76 (MQ=255) ctcAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACct < 1:1092191/76‑1 (MQ=255) ctcAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACct < 2:515489/76‑1 (MQ=255) ctcAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACct < 1:896745/76‑1 (MQ=255) cAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACctct > 1:488001/1‑76 (MQ=255) cAGGAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACctct > 1:487995/1‑76 (MQ=255) ggAATTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACctctct < 1:487286/76‑1 (MQ=255) aTTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCtt > 2:294258/1‑74 (MQ=255) aTTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCtt < 1:294258/74‑1 (MQ=255) aTTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTtat < 2:698721/76‑1 (MQ=255) aTTGCTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTtat < 2:874275/76‑1 (MQ=255) | GTCGCCTTTTTCAACTTTCCGATTAAGAACCTGCTCAGCGGGTTCTTGCTGTTTGTACTTTGTCTCAGGAATTACTTACGAAGCATAACTTCGATAAAGTCTTTCCAGTTCCCCAGTTCACGTTCAATCATAACAACCTCTCTTAT > REL606/2511564‑2511709 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |