Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,358,409	T→A	I24N (ATC→AAC)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,358,409	0	T	A	100.0%	152.7 / NA	40	I24N (ATC→AAC)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (23/17);  total (23/17)

CCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGA  >  REL606/2358337‑2358481                                                                         |                                                                         ccGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCg                                                                       >  2:43508/1‑76 (MQ=255)   gtgtCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTg                                                                     <  1:93730/76‑1 (MQ=255)    tgtCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGc                                                                    >  2:293230/1‑76 (MQ=255)       cccGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAAt                                                                 >  1:318578/1‑76 (MQ=255)          gTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGa                                                              <  1:1183481/76‑1 (MQ=255)           tattattatGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGaa                                                             >  1:475678/1‑76 (MQ=255)           tattattatGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGaa                                                             >  1:598604/1‑76 (MQ=255)               attatGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCa                                                         <  1:929054/76‑1 (MQ=255)               attatGCTGAGCCCTACAGGAGACAGCGTCGGTCTGCCCAGTGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCa                                                         <  1:929068/76‑1 (MQ=255)                 tatGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCaaa                                                       >  1:623766/1‑76 (MQ=255)                   tGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAgg                                                     <  2:1066363/76‑1 (MQ=255)                    gCTGATCCCTACCGGAACCAGCGTCGGACTGACCAGCGTAAGCCTTAGCGTGAACCGAGCAATGGAACGCAAAGGc                                                    >  1:164904/1‑76 (MQ=255)                       gATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGtt                                                 >  2:371354/1‑76 (MQ=255)                       gATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGtt                                                 <  1:1098928/76‑1 (MQ=255)                       gATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGtt                                                 >  1:931455/1‑76 (MQ=255)                        aTCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTc                                                <  2:968990/76‑1 (MQ=255)                         tCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCg                                               >  2:850694/1‑76 (MQ=255)                          cccTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGt                                              >  2:927839/1‑76 (MQ=255)                           ccTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTc                                             >  2:327632/1‑76 (MQ=255)                            cTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCt                                            <  2:1167620/76‑1 (MQ=255)                            cTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCt                                            >  2:955596/1‑76 (MQ=255)                              aCCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGa                                          >  1:900179/1‑76 (MQ=255)                               ccGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAg                                         <  2:636565/76‑1 (MQ=255)                                  gAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGt                                      >  1:499964/1‑76 (MQ=255)                                   aaCCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGtt                                     <  2:921581/76‑1 (MQ=255)                                     ccAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGtttt                                   <  1:441646/76‑1 (MQ=255)                                      cAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTc                                  >  2:1047669/1‑76 (MQ=255)                                               tCTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATc                         <  2:1049347/76‑1 (MQ=255)                                                cTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCg                        <  2:305860/76‑1 (MQ=255)                                                cTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCg                        <  1:575894/76‑1 (MQ=255)                                                cTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCg                        <  1:628698/76‑1 (MQ=255)                                                cTGACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCg                        >  1:6821/1‑76 (MQ=255)                                                  gACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCt                      >  1:127814/1‑76 (MQ=255)                                                  gACCAGCGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCt                      <  2:796581/76‑1 (MQ=255)                                                        cGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCcg                >  2:958246/1‑76 (MQ=255)                                                        cGTCAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCcg                >  2:825125/1‑76 (MQ=255)                                                           cAGCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGt             >  1:759249/1‑76 (MQ=255)                                                             gCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTTTCGCTCAGCCGCGTAc           <  2:183185/76‑1 (MQ=255)                                                             gCCTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTAc           <  2:943526/76‑1 (MQ=255)                                                              ccTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTAcc          >  1:46365/1‑76 (MQ=255)                                                               cTTGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCg         >  2:878427/1‑76 (MQ=255)                                                                ttGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCgg        <  1:993873/76‑1 (MQ=255)                                                                ttGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTCCAAACCTATCGCTCAGCCGCGTACCgg        >  2:877397/1‑76 (MQ=255)                                                                 tGGCGTGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGt       >  2:877673/1‑76 (MQ=255)                                                                      tGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGa  >  2:639716/1‑76 (MQ=255)                                                                      tGAACCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGa  >  2:1154556/1‑76 (MQ=255)                                                                         |                                                                         CCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGA  >  REL606/2358337‑2358481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑