Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,928,260	T→A	I15N (ATC→AAC)	rho →	transcription termination factor Rho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,928,260	0	T	A	100.0%	154.0 / NA	41	I15N (ATC→AAC)	rho	transcription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/27);  total (14/27)

TTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTT  >  REL606/3928187‑3928324                                                                          |                                                                 tttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACa                                                                <  2:512647/76‑1 (MQ=255) tttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACa                                                                >  1:554596/1‑76 (MQ=255)    aCCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACActc                                                             <  2:815638/76‑1 (MQ=255)    aCCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACActc                                                             <  2:128942/76‑1 (MQ=255)      cccAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACtctc                                                           <  1:1197751/76‑1 (MQ=255)      cccAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACtctc                                                           >  1:987132/1‑76 (MQ=255)       ccAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCg                                                          <  1:393203/76‑1 (MQ=255)              tAAGAATTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGaaaa                                                   >  2:699864/1‑76 (MQ=255)              tAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGaaaa                                                   >  2:699872/1‑76 (MQ=255)               aaGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAat                                                  <  1:718966/76‑1 (MQ=255)                aGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAata                                                 >  2:206028/1‑76 (MQ=255)                 gAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAatat                                                <  1:314147/76‑1 (MQ=255)                  aaCTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATg                                               >  2:590519/1‑76 (MQ=255)                    cTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATggg                                             <  2:860969/76‑1 (MQ=255)                     tCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATgggg                                            >  1:872735/1‑76 (MQ=255)                     tCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATgggg                                            >  1:872744/1‑76 (MQ=255)                            acTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGaaa                                     <  2:607630/76‑1 (MQ=255)                            acTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGaaa                                     <  2:300280/76‑1 (MQ=255)                              tATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAAc                                   >  2:263277/1‑76 (MQ=255)                                tGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCt                                 <  2:598267/76‑1 (MQ=255)                                tGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCt                                 <  2:192350/76‑1 (MQ=255)                                tGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCt                                 <  2:192357/76‑1 (MQ=255)                                    tCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCt                             >  2:1101859/1‑76 (MQ=255)                                    tCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCt                             <  2:529545/76‑1 (MQ=255)                                     cTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTc                            <  1:200258/76‑1 (MQ=255)                                       tACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGt                          <  1:590732/76‑1 (MQ=255)                                       tACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGt                          <  1:590726/76‑1 (MQ=255)                                         ccGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTAt                        <  1:751691/76‑1 (MQ=255)                                          cGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATg                       <  2:468888/76‑1 (MQ=255)                                            aaTTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCg                     >  2:439791/1‑76 (MQ=255)                                             aTTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGt                    <  2:924135/76‑1 (MQ=255)                                             aTTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGt                    >  1:608214/1‑76 (MQ=255)                                             aTTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGt                    >  1:608210/1‑76 (MQ=255)                                              ttAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTa                   >  1:616044/1‑76 (MQ=255)                                               tAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTaa                  <  2:606560/76‑1 (MQ=255)                                               tAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTaa                  <  2:606562/76‑1 (MQ=255)                                                aaaGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAg                 <  2:204631/76‑1 (MQ=255)                                                 aaGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGc                <  2:302712/76‑1 (MQ=255)                                                  aGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCa               <  2:1115242/76‑1 (MQ=255)                                                       aCGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACa          >  1:439527/1‑76 (MQ=255)                                                        cGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACat         <  2:52682/76‑1 (MQ=255)                                                         gCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACatt        <  2:880076/76‑1 (MQ=255)                                                           cGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACattat      <  2:458356/76‑1 (MQ=255)                                                             gTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTAttt    <  1:1004469/76‑1 (MQ=255)                                                              tttCTGAGCTGAACACTCTCGGCGAAACTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTAtttt   >  2:7800/1‑76 (MQ=255)                                                               ttCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTAttttt  <  1:518638/76‑1 (MQ=255)                                                                          |                                                                 TTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTT  >  REL606/3928187‑3928324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑