| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,920,708 | G→A | A151V (GCG→GTG) | znuA ← | high‑affinity zinc transporter periplasmic component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,920,708 | 0 | G | A | 97.4% | 139.9 / ‑6.5 | 38 | A151V (GCG→GTG) | znuA | high‑affinity zinc transporter periplasmic component |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (19/18); minor base C (1/0); total (20/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GGCGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCGCTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTT > REL606/1920635‑1920772 | ggCGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACt < 2:1076492/76‑1 (MQ=255) cGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTAt < 1:1154716/76‑1 (MQ=255) cGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTAt < 2:822234/76‑1 (MQ=255) cAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTAtctc < 2:610241/76‑1 (MQ=255) cAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTAtctc < 2:610235/76‑1 (MQ=255) cAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTAtctc < 1:277622/76‑1 (MQ=255) cAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTAtctc > 1:687564/1‑76 (MQ=255) aaGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATctct < 2:782236/76‑1 (MQ=255) gtgtGGCTCGACTTTGTGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTgg < 1:201474/73‑1 (MQ=255) gTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTgg < 1:201490/76‑1 (MQ=255) tttGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTggg < 2:1065980/76‑1 (MQ=255) ggCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGaa < 2:898378/76‑1 (MQ=255) ggCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGaa < 2:1010468/76‑1 (MQ=255) cGACTTTGCGGCATAAGTTCCACTAATTTTCCATGAATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGcc > 1:124725/1‑76 (MQ=255) cGACTTTGCGGCATAAGTTCCACTAATTTTCCATGAATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGcc > 1:124717/1‑76 (MQ=255) tttGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAg < 1:647097/76‑1 (MQ=255) tGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCCCTATCTCTGGGGAAAGCCAAAGAt > 2:824033/1‑76 (MQ=255) tGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGAt > 2:824038/1‑76 (MQ=255) tGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGAt < 1:986332/76‑1 (MQ=255) cGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGc > 2:535237/1‑76 (MQ=255) tAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGtt < 1:630702/76‑1 (MQ=255) tAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGtt > 1:470763/1‑76 (MQ=255) aaGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTg > 1:281213/1‑76 (MQ=255) aaGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTg > 2:1086574/1‑76 (MQ=255) aGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGa > 1:1175480/1‑76 (MQ=255) ttCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGaaa > 1:280643/1‑76 (MQ=255) cACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCg < 1:155554/76‑1 (MQ=255) tAATTTTCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCg > 2:1058270/1‑76 (MQ=255) ttttCCATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGa > 1:995897/1‑76 (MQ=255) ccATGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGt > 1:221443/1‑76 (MQ=255) aTGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGa < 1:276776/76‑1 (MQ=255) tGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGAt > 2:654600/1‑76 (MQ=255) tGGATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGAt < 1:1042495/76‑1 (MQ=255) gATTGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCt > 1:647747/1‑76 (MQ=255) tGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCg > 2:1216375/1‑76 (MQ=255) tGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCg > 1:340641/1‑76 (MQ=255) tGCAACCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCg > 1:340633/1‑76 (MQ=255) aaCCGCTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCa > 1:462778/1‑76 (MQ=255) cTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACtttt > 2:738636/1‑76 (MQ=255) cTGTAGCCCGCACTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACtttt > 1:577049/1‑76 (MQ=255) | GGCGTCAAGTTTGGCTCGACTTTGCGGCATAAGTTCCACTAATTTTCCATGGATTGCAACCGCTGTAGCCCGCGCTATCTCTGGGGAAAGCCAAAGATGCATGTTGAAATCGCCGTGATGGTGATCTTCGTCACTTTT > REL606/1920635‑1920772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |