Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,336,054	G→A	A171V (GCA→GTA)	nuoM ←	NADH dehydrogenase subunit M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,336,054	0	G	A	100.0%	81.9 / NA	23	A171V (GCA→GTA)	nuoM	NADH dehydrogenase subunit M
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/13);  total (10/13)

ACCAGCGCCAGGATGGCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCAC  >  REL606/2335988‑2336124                                                                   |                                                                       aCCAGCGCCAGGATGGCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGa                                                               >  1:839882/1‑76 (MQ=255)  ccAGCGCCAGGATGGCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGAt                                                              >  2:221810/1‑76 (MQ=255)  ccAGCGCCAGGATGGCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGAt                                                              <  1:843705/76‑1 (MQ=255)           ggATGGCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGtttt                                                     <  2:823789/76‑1 (MQ=255)             aTGGCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTAc                                                   >  1:1136531/1‑76 (MQ=255)             aTGGCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTAc                                                   >  2:247468/1‑76 (MQ=255)               ggCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCg                                                 <  1:1191843/76‑1 (MQ=255)                        cATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCt                                        <  1:128294/76‑1 (MQ=255)                            aCCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTAt                                    <  2:66801/76‑1 (MQ=255)                                gACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGccc                                <  1:1110794/76‑1 (MQ=255)                                   cACTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCca                             <  1:343265/76‑1 (MQ=255)                                    aCTCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCcac                            <  1:963887/76‑1 (MQ=255)                                      tCGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAg                          <  2:931968/76‑1 (MQ=255)                                       cGCCTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGt                         >  2:55104/1‑76 (MQ=255)                                         ccTGGGTGTAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGc                       <  1:346175/76‑1 (MQ=255)                                               tgtAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAg                 <  2:1206013/76‑1 (MQ=255)                                               tgtAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAg                 >  1:58908/1‑76 (MQ=255)                                                gtAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAgg                >  1:616277/1‑76 (MQ=255)                                                 tAAATGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGa               >  2:367583/1‑76 (MQ=255)                                                   aaTGAAGAACTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAg             >  1:616213/1‑76 (MQ=255)                                                        agaaCTTGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACAt        <  2:484522/76‑1 (MQ=255)                                                           aCTTGGTTACCGCCGTGATACGCGTTTTACCGTTAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCgg     <  2:579119/76‑1 (MQ=255)                                                           aCTTGGTTACCGCCGTGATAAGCGTTTTAACGTCAGAGGCTTTATGCCAAAACAGTGAGATCAGGAAGTACATCgg     >  1:988647/1‑76 (MQ=255)                                                              tGGTTACCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATAAGGAAGAACATCGGCAc  >  1:173764/1‑76 (MQ=255)                                                                   |                                                                       ACCAGCGCCAGGATGGCAATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCAC  >  REL606/2335988‑2336124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑