| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,202,194 | +C | coding (366/825 nt) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,202,192 | 1 | . | C | 97.7% | 175.8 / ‑2.8 | 43 | coding (368/825 nt) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (17/25); total (18/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCG‑CCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAGGGTGAACAATCGCTAACAAATT > REL606/4202117‑4202262 | gcgGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCg < 2:98120/76‑1 (MQ=255)gcgGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCg > 2:82173/1‑76 (MQ=255) ggACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCG‑cc > 1:1024578/1‑76 (MQ=255) gACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCG‑ccc < 2:1399803/76‑2 (MQ=255) gCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGa < 1:802392/76‑1 (MQ=255) cAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGt > 1:1518956/1‑76 (MQ=255) aGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGtt < 2:918667/76‑1 (MQ=255) aGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGtt > 1:445865/1‑76 (MQ=255) tGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCg < 2:94426/76‑1 (MQ=255) gTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCa < 1:728400/76‑1 (MQ=255) acacTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGa > 2:333878/1‑76 (MQ=255) cacTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGAc < 1:1278077/76‑1 (MQ=255) acTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGAct < 2:249246/76‑1 (MQ=255) acTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGAct < 2:653784/76‑1 (MQ=255) tGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGActct > 1:102096/1‑76 (MQ=255) tACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTc > 1:1452402/1‑76 (MQ=255) aCCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTcc > 2:1314125/1‑76 (MQ=255) ccTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCa < 2:1432272/76‑1 (MQ=255) tGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCAtt < 1:310347/76‑1 (MQ=255) tCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTaaa < 2:523059/76‑1 (MQ=255) cGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAAt > 2:1321425/1‑76 (MQ=255) gATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAAtt < 2:75901/76‑1 (MQ=255) gATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAAtt < 1:276730/76‑1 (MQ=255) ataatCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTgcgc > 2:1073386/1‑76 (MQ=255) atCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAgg < 1:320145/76‑1 (MQ=255) atCGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAgg < 1:806166/76‑1 (MQ=255) cGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGAt < 2:1474904/76‑1 (MQ=255) cGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGAt < 1:1379452/76‑1 (MQ=255) cGCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGAt < 1:569517/76‑1 (MQ=255) gCTTCGTGATCGCTTTGATCCAGCCCCGCCCATATTGTCCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATa < 2:141685/76‑1 (MQ=255) gCTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATa < 2:1270705/76‑1 (MQ=255) cTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAg < 2:955446/76‑1 (MQ=255) cTTCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAg > 2:1234570/1‑76 (MQ=255) ttCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAgg > 2:1216338/1‑76 (MQ=255) ttCGTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAgg > 1:82372/1‑76 (MQ=255) ctCGCGACCGCTTTGATCAAGCACCG‑CCATATTGACCTTTTCGCCACACTCTTCCATTAAATTCCGCACGATAggc > 2:1179073/2‑75 (MQ=255) gTGATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAGGGTg < 1:1255498/76‑1 (MQ=255) gATCGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAGGGTGaa > 2:295688/1‑76 (MQ=255) cGCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAGGGTGAACaa > 1:111920/1‑76 (MQ=255) gCTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAGGGTGAACAAt > 2:147824/1‑76 (MQ=255) cTTTGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAGGGTGAACAAta > 1:428867/1‑75 (MQ=255) tttGATCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAGGGTGAACAATCg > 1:1333678/1‑76 (MQ=255) aTCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAGGGTGAACAATCGCTaa < 1:920170/76‑1 (MQ=255) aTCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAGGGTGAACAATCGCTaa < 1:1058620/76‑1 (MQ=255) aTCAAGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAGGGTGAACAATCGCTaa < 1:596790/76‑1 (MQ=255) aTCAAGCACCGCCCATATTGACCGTTACGCCAGACTCTTCCATTAAATTGCGCAGGATAGGGTGAACAATCGCTaa < 1:596796/76‑1 (MQ=255) aaGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAGGGTGAACAATCGCTAACaa > 2:1272601/1‑76 (MQ=255) aGCACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAGGGTGAACAATCGCTAACaaa < 2:762545/76‑1 (MQ=255) cACCGCCCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAGGGTGAACAATCGCTAACAAAtt < 1:669068/76‑1 (MQ=255) | GCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCG‑CCATATTGACCGTTTCGCCAGACTCTTCCATTAAATTGCGCAGGATAGGGTGAACAATCGCTAACAAATT > REL606/4202117‑4202262 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |