New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 242771	NA (NA)	8 (0.180)	6/140	4.5	11.2%	intergenic (+389/‑42)	ECB_00212/yhhI	hypothetical protein/predicted transposase
	?	REL606	= 719226	68 (1.420)					coding (242/762 nt)	ybfD	hypothetical protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAGATGATAAAGACGTCA  >  REL606/719153‑719300                                                                          |                                                                           tttttGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCt                                                                          >  2:1325853/1‑76 (MQ=31)   tttGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGt                                                                        >  1:262793/1‑76 (MQ=34)     tGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTcc                                                                      >  2:899279/1‑76 (MQ=34)     tGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTcc                                                                      >  2:1489616/1‑76 (MQ=34)       aaGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTg                                                                    <  1:769923/76‑1 (MQ=34)       aaGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTg                                                                    <  2:812835/76‑1 (MQ=34)       aaGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTg                                                                    <  1:769949/76‑1 (MQ=34)            atatGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGaaa                                                               <  1:311065/76‑1 (MQ=39)                ggTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTc                                                           >  1:403722/1‑76 (MQ=39)                 ntGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCa                                                          >  1:335823/2‑76 (MQ=39)                   gATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATg                                                        >  1:1467682/1‑76 (MQ=255)                        tGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGc                                                   >  1:1293810/1‑76 (MQ=255)                           aaaTGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCttt                                                <  2:623382/76‑1 (MQ=255)                            aaTGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTtta                                               >  1:446051/1‑76 (MQ=255)                             aTGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTttat                                              <  1:65499/76‑1 (MQ=255)                              tGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTttatt                                             >  1:1394806/1‑76 (MQ=255)                              tGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTttatt                                             >  2:928533/1‑76 (MQ=255)                              tGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTttatt                                             <  1:73970/76‑1 (MQ=255)                                   ttCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTg                                        <  1:1533914/76‑1 (MQ=255)                                    tCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTgg                                       >  1:1471376/1‑76 (MQ=255)                                    tCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTgg                                       >  2:90804/1‑76 (MQ=255)                                     ccTGTTCACGATCCCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGTTTTATTAACTGGa                                      <  2:963456/76‑1 (MQ=255)                                     ccTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGa                                      >  2:871686/1‑76 (MQ=255)                                     ccTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCAGGAGGGCTTTATTACCTGGa                                      <  2:963475/76‑1 (MQ=14)                                        gTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGc                                   >  1:1485793/1‑76 (MQ=255)                                        gTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGc                                   >  2:1315186/1‑76 (MQ=255)                                         ttCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCt                                  >  1:1144960/1‑76 (MQ=255)                                         ttCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCt                                  >  1:219351/1‑76 (MQ=255)                                          tCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCtt                                 <  1:1319385/76‑1 (MQ=255)                                           cACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTg                                >  1:56049/1‑76 (MQ=255)                                           cACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTg                                >  2:302166/1‑76 (MQ=255)                                           cACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTg                                <  1:805876/76‑1 (MQ=255)                                           cACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTg                                >  2:159907/1‑76 (MQ=255)                                            aCGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGa                               >  1:33929/1‑76 (MQ=255)                                              gATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACt                             <  1:880110/76‑1 (MQ=255)                                               aTACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTa                            <  1:321034/76‑1 (MQ=255)                                               aTACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTa                            <  2:1324112/76‑1 (MQ=255)                                                tACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTAc                           >  2:528327/1‑76 (MQ=255)                                                 aCCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTAcc                          >  1:966203/1‑76 (MQ=255)                                                 aCCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTAcc                          <  1:275150/76‑1 (MQ=255)                                                  ccATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCa                         >  1:597973/1‑76 (MQ=255)                                                  ccATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCa                         >  2:65577/1‑76 (MQ=255)                                                    aTTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCAtt                       >  1:971889/1‑76 (MQ=255)                                                    aTTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCAtt                       <  2:1039768/76‑1 (MQ=255)                                                     ttGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCAttc                      >  1:1365370/1‑76 (MQ=255)                                                     ttGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCAttc                      >  2:1129651/1‑76 (MQ=255)                                                     ttGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCAttc                      >  1:490291/1‑76 (MQ=255)                                                      tGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCAttct                     <  1:839197/76‑1 (MQ=255)                                                      tGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCAttct                     <  1:839175/76‑1 (MQ=255)                                                       gCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCAttctt                    <  1:127556/76‑1 (MQ=255)                                                       gCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCAttctt                    <  1:591347/76‑1 (MQ=255)                                                       gCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCAttctt                    <  2:495259/76‑1 (MQ=255)                                                         cAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCa                  <  1:306308/76‑1 (MQ=255)                                                          agagTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAg                 <  1:1291612/76‑1 (MQ=255)                                                          agagTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAg                 <  1:1105220/76‑1 (MQ=255)                                                          agagTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAg                 >  1:965970/1‑76 (MQ=255)                                                            agTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAgat               >  1:830433/1‑76 (MQ=255)                                                            agTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAgat               <  1:304444/76‑1 (MQ=255)                                                             gTTGTATCCTGTATCTATCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAgatg              <  1:961860/76‑1 (MQ=255)                                                               tGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAgatgat            >  1:759313/1‑76 (MQ=255)                                                                 tATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAGATGATaa          >  1:1054062/1‑76 (MQ=255)                                                                 tATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAGATGATaa          >  1:170271/1‑76 (MQ=255)                                                                 tATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAGATGATaa          <  1:184282/76‑1 (MQ=255)                                                                   tCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAGATGATAAAg        <  1:296886/76‑1 (MQ=255)                                                                    ccTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAGATGATAAAGa       >  2:586800/1‑76 (MQ=255)                                                                    ccTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAGATGATAAAGa       <  2:1405883/76‑1 (MQ=255)                                                                      tGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAGATGATAAAGACg     >  2:662623/1‑76 (MQ=255)                                                                         aTCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAGATGATAAAGACGTCa  >  1:955047/1‑76 (MQ=255)                                                                          |                                                                           TTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGATACCATTGCCAGAGTTGTATCCTGTATCTGTCCTGCGAAATTTCATGAGAGCTTTATTAACTGGATGCTTGACTACCATTCTTCAGATGATAAAGACGTCA  >  REL606/719153‑719300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.