| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,193,166 | 0 | G | C | 36.8% | 27.5 / 11.7 | 19 | A347G (GCG→GGG) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (11/1); new base C (0/7); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.59e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTAACTGACGCCAGAGCCATTCCACCGG‑CCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATT‑CGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTA > REL606/2193092‑2193236 | ttAACTGACGCCAGAGCCATTCCACCGG‑CCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATT‑Cgc < 2:588974/76‑1 (MQ=255) gACGCCAGAGCCATTCCACCGG‑CCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATT‑Cgccagcca < 2:564263/76‑1 (MQ=255) aCGCCAGAGCCATTCCCCCGG‑CCCCGGGGGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATT‑CGCCAGCCAt < 1:649666/76‑1 (MQ=255) ccccAGAGCCGCCCCCCCGG‑CCCCGGGCGGAAGTAACGCAGCCAGAAAAAAGAGAAGAGGATATC‑CCCCAGCCATa < 2:64452/74‑1 (MQ=255) aggCCCTTCCCCCGGCCCCCGGGGGGAAGAACCGCAGCAAAAAAACAGAGAAGAGGATATC‑CCCCAGCCATACCgg < 2:827866/74‑1 (MQ=255) gg‑CCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATT‑CGCCAGCCATACCGGAATAACAAACGCca > 1:1423170/1‑76 (MQ=255) cccTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATT‑CGCCAGCCATACCGGAATAACACACGCcagca > 1:382702/1‑76 (MQ=255) ggggAAGAAACGCAGCAAAAAAAAAGAAAAGAGGATATC‑CCCCAGCCATACCGGAATAACAAACGCCAGCAGCTcc < 1:73726/74‑1 (MQ=255) gaagaaACGCAGCAAGAAAAAAGAGAAGAGGATATC‑CCCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAgg < 1:1470470/76‑1 (MQ=255) aaaGAAACGCACCAAGAAAAAAGA‑AAGGGGATTTTCCCCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAgg < 1:979486/75‑1 (MQ=255) aaGAAACGAAGCAAAAAAAAAGAAAAGGGTATTTC‑CCCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGc < 1:618602/76‑1 (MQ=255) tAACGCAGCCAGATAACAGAGAAGAGGATATT‑CGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGt > 2:177280/1‑76 (MQ=255) aaaCGCAGCCAAAAAACAGAGAAGAGGATATT‑CCCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGt < 2:1400296/75‑1 (MQ=255) aaCGCAGCCAGATAACAGAGAAGAGGATATT‑CGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTc > 2:68566/1‑76 (MQ=255) aGCAAGAAAAAAGAGAAGGGCATATC‑CCCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAAt < 2:1358820/76‑1 (MQ=255) gCCAAATAAGAGAGGAGAGGATATT‑CCCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATg < 1:477081/76‑1 (MQ=255) ccAGATAACAGAGAAGAGGATATT‑CGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGc > 2:1326533/1‑76 (MQ=255) aGATAACAGAGAAGAGGATATT‑CGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCAt > 1:48493/1‑76 (MQ=255) aTAACAGAGAAGAGGATATT‑CGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATaa > 2:459384/1‑76 (MQ=255) aTAACAGAGAAGAGGATATT‑CGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATaa > 1:576767/1‑76 (MQ=255) aCAGAGAAGAGGATATT‑CGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATaaaca > 1:781697/1‑76 (MQ=255) aagaGGATATT‑CGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGa > 1:1139605/1‑76 (MQ=255) aagaGGATATC‑CCCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGa < 1:1396726/76‑1 (MQ=255) tatT‑CGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTa > 2:1005797/1‑76 (MQ=255) | TTAACTGACGCCAGAGCCATTCCACCGG‑CCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATT‑CGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTA > REL606/2193092‑2193236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |