| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,007,369 | A→T | L154* (TTG→TAG) | ECB_02804 ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,007,369 | 0 | A | T | 97.3% | 136.2 / ‑3.3 | 37 | L154* (TTG→TAG) | ECB_02804 | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (17/19); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01 | |||||||||||
TTACGCCCTGCAGCATCCGGAATGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCAACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGTCTCATTCTGCGGGCCCTGAAAACCC > REL606/3007298‑3007440 | ttACGCCCTGCAGCATCCGGAATGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGctacaa < 2:980646/76‑1 (MQ=255)ttACGCCCTGCAGCATCCGGAATGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGctacaa < 1:516107/76‑1 (MQ=255)ttACGCCCTGCAGCATCCGGAATGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGctacaa > 1:737570/1‑76 (MQ=255)ttACGCCCTGCAGCATCCGGAATGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGctacaa > 2:1229531/1‑76 (MQ=255)ttACGCCCTGCAGCATCCGGAATGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGctacaa > 2:97601/1‑76 (MQ=255) tACGCCCTGCAGCATCCGGAATGCCGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCtacaac > 2:8865/1‑76 (MQ=255) tACGCCCTGCAGCATCCGGAATGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCtacaac > 2:1336322/1‑76 (MQ=255) tACGCCCTGCAGCATCCGGAATGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCtacaac > 1:264395/1‑76 (MQ=255) cccTGCAGCATCCGGAATGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAActttc < 1:931849/76‑1 (MQ=255) cTGCAGCATCCGGAATGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAActttcct > 1:281708/1‑76 (MQ=255) tGCAGCATCCGGAATGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAActttcctt < 1:1281658/76‑1 (MQ=255) gcagcaTCCGGAATGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAActttccttt < 2:170219/76‑1 (MQ=255) agcaTCCGGAATGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCt < 2:776778/76‑1 (MQ=255) ccGGAATGCAGCGCCATTCTGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCg > 2:205632/1‑76 (MQ=255) gAATGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAg < 2:1073574/76‑1 (MQ=255) aaTGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGc > 1:1264360/1‑76 (MQ=255) aaTGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGATACAAATTTCATTTCTGCCAGGAga > 2:1525791/1‑75 (MQ=255) aTGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCg > 2:827107/1‑76 (MQ=255) cAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATg > 2:26247/1‑76 (MQ=255) aGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGa < 2:983292/76‑1 (MQ=255) gcCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGc < 1:428036/76‑1 (MQ=255) cATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGccc < 1:1486945/76‑1 (MQ=255) aTTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCg > 1:292719/1‑76 (MQ=255) tATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGc > 1:1049985/1‑76 (MQ=255) aTGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCg > 1:118093/1‑76 (MQ=255) aTGCGCATCATCGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCg < 1:1085754/76‑1 (MQ=255) cgcATCATCGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAgc < 2:1220473/76‑1 (MQ=255) gcATCATCGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAgcg > 2:923755/1‑76 (MQ=255) tcatcGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAg < 2:1348366/76‑1 (MQ=255) atcGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGtc < 2:406303/76‑1 (MQ=255) tcGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGtct < 2:977839/76‑1 (MQ=255) cGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGtctc < 1:1507216/76‑1 (MQ=255) cGACTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGtctc > 1:1366214/1‑76 (MQ=255) naCTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGTCTCa > 1:1098359/2‑76 (MQ=255) cTGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGTCTCAtt > 1:466213/1‑76 (MQ=255) tGAATGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGTCTCATTc < 1:380772/76‑1 (MQ=255) aaTGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGTCTCATTCTg < 1:215064/76‑1 (MQ=255) aaTGGAGAGAAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGTCTCATTCTg < 2:756716/76‑1 (MQ=255) agaAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGTCTCATTCTGCGGGccc > 1:1421501/1‑76 (MQ=255) agaAGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGTCTCATTCTGCGGGccc < 1:114859/76‑1 (MQ=255) agaAGCACAATGCAACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGTCTCATTCTGCGGGccc > 2:475839/1‑76 (MQ=255) aGCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGTCTCATTCTGCGGGCCCTGa > 2:284277/1‑76 (MQ=255) gCACAATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGTCTCATTCTGCGGGCCCTGaa > 1:1520293/1‑76 (MQ=255) cacaATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGTCTCATTCTGCGGGCCCTGaca > 2:285963/1‑74 (MQ=255) cacaATGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGTCTCATTCTGCGGGCCCTGaaa > 2:407595/1‑76 (MQ=255) aaTGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGTCTCATTCTGCGGGCCCTGAAAAcc < 1:511265/76‑1 (MQ=255) aTGCTACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGTCTCATTCTGCGGGCCCTGAAAAccc > 1:612891/1‑76 (MQ=255) | TTACGCCCTGCAGCATCCGGAATGCAGCGCCATTATGCGCATCATCGACTGAATGGAGAGAAGCACAATGCAACAACTTTCCTTTCTGCCCGGAGCGATGACGCCCGGCGAGCGCAGTCTCATTCTGCGGGCCCTGAAAACCC > REL606/3007298‑3007440 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |