Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	474,234	Δ4 bp	coding (1069‑1072/1677 nt)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 474233	2 (0.040)	46 (0.850)	39/150	0.3	95.8%	coding (1073/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
	?	REL606	474238 =	2 (0.040)					coding (1068/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

GGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/474165‑474233 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTA  >  REL606/474238‑474310                                                                                                                                                GGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCC                                                                    <  1:1411125/76‑1   TTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAG                                                                  >  2:551690/1‑76    TGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGT                                                                 >  1:305972/1‑76      AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCC                                                               <  2:787395/76‑1      AGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCC                                                               <  1:1586119/76‑1       GCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCC                                                              <  1:14005/76‑1          TAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCC                                                           <  1:264375/76‑1             cCGACGGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGG                                                        <  1:1246056/75‑1             TCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGG                                                        <  1:4954/76‑1             TCGACAGGATCGCCCCTGCCAGTACCAGGTTTTATCCCGCCTGCGGCAGTAAAATCACAAACCCACACCCGCCAGa                                                        >  1:1063867/1‑75               GACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGAT                                                      >  1:1545258/1‑76               GACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGAT                                                      <  2:1624450/76‑1               GACAGGACCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGAT                                                      <  2:1749849/76‑1                 CAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAA                                                    >  1:1740526/1‑76                 CAGGACCCCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAA                                                    <  2:396178/76‑1                   GGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAAC                                                  <  1:1431590/76‑1                     ATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGC                                                >  1:1618464/1‑76                     ATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGAGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGC                                                >  2:1583385/1‑76                      TCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCG                                               <  2:606718/76‑1                        GCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAA                                             >  2:664374/1‑76                          CCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCATGATAAACGCGAACT                                           <  1:944980/76‑1                          CCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACT                                           >  2:1164535/1‑76                           CCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTC                                          >  2:1379119/1‑76                            CTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATACACGCGATCTCA                                         >  2:1529178/1‑76                             TGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCAC                                        >  2:47799/1‑76                              GCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACC                                       >  1:1000562/1‑76                               CCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCA                                      <  2:437734/76‑1                               CCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCA                                      <  1:1382679/76‑1                                CAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAA                                     <  1:1711299/76‑1                                   TACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCT                                  >  2:1270193/1‑76                                   TACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCT                                  <  2:197925/76‑1                                      CAGGTTTAGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCG                               >  1:1125426/1‑76                                          TTTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAG                           >  2:654839/1‑76                                           TTTGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGG                          >  2:49791/1‑76                                             nGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCT                        >  1:1567848/2‑76                                             TGTCCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCT                        >  1:368290/1‑76                                                CCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGC                     >  1:237312/1‑76                                                CCGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGC                     >  1:1247208/1‑76                                                 CGGCCTGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCG                    >  1:414717/1‑76                                                      TGCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGAT               >  2:822738/1‑76                                                       GCGGCAGTAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATG              >  2:931746/1‑76                                                           CAGTAAATTCACATTCCCAGTCCTGCCAGGATAAACGCGAACTCACCAATCGGCGCCAGGCTGGCGGCGATGGTTA          <  2:889318/76‑1                                                              TAAATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATG       <  2:821958/76‑1                                                                AATTCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCC     >  1:236447/1‑76                                                                   TCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTA  <  2:898392/76‑1                                                                   TCACATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTA  >  2:1550868/1‑76                                                                                                                                                GGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/474165‑474233 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTA  >  REL606/474238‑474310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.