| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,492,082 | 0 | T | G | 28.1% | 125.2 / 13.1 | 57 | V490G (GTG→GGG) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (9/32); new base G (16/0); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.53e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTGACC > REL606/3492007‑3492157 | acgCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggt < 2:460585/76‑1 (MQ=255) cccTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGa < 1:253261/76‑1 (MQ=255) ccTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggggggg > 1:1616695/1‑74 (MQ=255) tggggCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGta < 1:1300926/73‑1 (MQ=255) gtgGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGtat < 1:274115/76‑1 (MQ=255) gtgGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGtat < 1:292079/76‑1 (MQ=255) gtgGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGtat < 2:801584/76‑1 (MQ=255) ggCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATAc > 1:674338/1‑76 (MQ=255) tCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACggcg > 1:90923/1‑73 (MQ=255) cGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgcagc < 1:1292717/76‑1 (MQ=255) cacaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGcagcag < 2:331567/76‑1 (MQ=255) acaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGATTATACGCAGCAGa < 1:1158765/76‑1 (MQ=255) caAAACAAGGCATGTGGCTAAATGCGGATCAACAAACTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGaa > 2:1582739/1‑76 (MQ=255) aCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTg < 1:1598984/76‑1 (MQ=255) cAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGATGCTgg > 2:1718657/1‑75 (MQ=255) aaGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGACAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTc < 2:1583736/76‑1 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCt < 2:1363850/76‑1 (MQ=255) cATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGGGTAAACGCAGCAGAAGCTGTTGCTg > 1:1740300/1‑76 (MQ=255) cATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGGGGATACGCAGCAGAAGCTGTCGCTg > 2:829604/1‑76 (MQ=255) aTGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGGGTATACGCAGCAGAAGCTGTCGCTgg > 1:1054747/1‑76 (MQ=255) aTGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTgg < 1:301912/76‑1 (MQ=255) aTGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTgg > 1:45612/1‑76 (MQ=255) tgtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGc < 1:110335/76‑1 (MQ=255) gtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGAAGCTGTCGCTGGcg > 2:578777/1‑76 (MQ=255) gtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGcg > 1:662383/1‑76 (MQ=255) tgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGAAGCTGTCGCTGGcgg > 1:1267597/1‑75 (MQ=255) ggCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGcgtc < 1:1731812/76‑1 (MQ=255) ggCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCCGCAGAAGCTGTCGCTGGcgcg > 1:1539301/1‑74 (MQ=255) ntAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGGGAATACGCAGCCGAAAAAGTCGCTGGggtggt > 1:670786/2‑76 (MQ=255) cTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGcgtcgt > 1:1648259/1‑76 (MQ=255) cTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGgcggcgg > 1:852452/1‑75 (MQ=255) cTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGggtcgt > 1:116973/1‑76 (MQ=255) tAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGGGTATACGCAGCAGAAGCTGTCGCTGgcggcggg > 1:1021798/1‑74 (MQ=255) tAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTg < 1:84084/76‑1 (MQ=255) tAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTg < 2:162022/76‑1 (MQ=255) tAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGGATACGCAGCAGAAGCTGTCGCTGGcgtcggg > 1:44607/1‑74 (MQ=255) aaaTGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGa > 1:1616831/1‑76 (MQ=255) aaaTGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGGATACGCAGCAGAAGATGTCGCTGGCGGCGTGa > 2:326903/1‑76 (MQ=255) aTGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGGGGATACGCAGCAGAAGCTGTCGCTGGCGTCGTGAtt > 1:1392860/1‑76 (MQ=255) cGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGGGTCGTGGTTTaa > 2:1159363/1‑76 (MQ=255) aTCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCGGGGGTGGGGGTTTTATGt > 1:253961/1‑76 (MQ=255) tCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTa < 2:54322/76‑1 (MQ=255) caacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTaa < 1:248112/76‑1 (MQ=255) caacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTaa < 1:1690925/76‑1 (MQ=255) caacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTaa < 2:1292158/76‑1 (MQ=255) caacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTaa < 2:1147747/76‑1 (MQ=255) caacaaTCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCCGTCGCCGGCGTCGCGGTTTAATGTaa > 2:360128/1‑76 (MQ=255) aacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAg < 1:1437320/76‑1 (MQ=255) aacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAg < 1:646356/76‑1 (MQ=255) aacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAg < 1:862754/76‑1 (MQ=255) aacaaTCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCCTGATTTAATGTAAg > 2:1659824/1‑76 (MQ=255) caaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAggg > 2:297225/1‑75 (MQ=255) caaTCTCGTGTGAGTCAGTGGCTGGTGGAGGATACGCAGCAGAAGCTGTTGCTGGCGGCGTGGTTTTATGTAAggg > 1:670512/1‑75 (MQ=255) aaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTg < 1:764513/76‑1 (MQ=255) aaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTg < 1:982540/76‑1 (MQ=255) tgtgAGTCAGTGGCCGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGt < 1:87708/76‑1 (MQ=255) gtgAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTc < 2:576979/76‑1 (MQ=255) gtgAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGGGGCCAgggc > 1:650510/1‑74 (MQ=255) gAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAg < 2:1100209/76‑1 (MQ=255) tCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGAtt < 2:1040745/76‑1 (MQ=255) cAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGAttt < 1:831440/76‑1 (MQ=255) cTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCt < 1:1601089/76‑1 (MQ=255) ggtggAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCATTCTGa > 2:1734854/1‑76 (MQ=255) tggATTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTGAcc < 2:576432/76‑1 (MQ=255) tggAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGGCCGAATTCAATCTTAcc > 1:114698/1‑76 (MQ=255) | ACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTGACC > REL606/3492007‑3492157 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |