Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	426,675	A→C	S280R (AGC→CGC)	tig →	trigger factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	426,675	0	A	C	100.0%	79.2 / NA	22	S280R (AGC→CGC)	tig	trigger factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/2);  total (20/2)

TTTCGGCGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACC  >  REL606/426602‑426745                                                                          |                                                                       tttCGGCGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAgcgc                                                                      <  2:691801/76‑1 (MQ=255) tttCGGCGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAgcgc                                                                      >  2:1471848/1‑76 (MQ=255)  ttCGGCGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGcgcg                                                                     <  2:1336562/76‑1 (MQ=255)  ttCGGCGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGcgcg                                                                     >  1:62410/1‑76 (MQ=255)   tCGGCGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAgcgcgc                                                                    >  2:956461/1‑76 (MQ=255)      gCGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCAt                                                                 <  2:745338/76‑1 (MQ=255)       cGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATc                                                                >  1:908018/1‑76 (MQ=255)       agTTGAAGATGGTTACGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATc                                                                <  2:1024133/75‑1 (MQ=255)        gTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATcc                                                               >  1:1544204/1‑76 (MQ=255)                 ggTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATCCGTAACcgcg                                                      >  2:1330555/1‑76 (MQ=255)                  gTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATCCGTAACcgcga                                                     >  1:419053/1‑75 (MQ=255)                      cGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATCCGTAACCGCGTTAAg                                                 >  1:1272587/1‑76 (MQ=255)                        tAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATCCGTAACCGCGTTAAGtc                                               >  2:541564/1‑76 (MQ=255)                        tAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATCCGTAACCGCGTTAAGtc                                               >  2:1616087/1‑76 (MQ=255)                        tAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATCCGTAACCGCGTTAAGtc                                               >  1:191659/1‑76 (MQ=255)                           gtggTCTGCGCGGTGAAGAGCAAAAAAGCGGGGGGCGCGGGCTGACGCGCCCCATCCGTAACCGCGTTAAGTCTCa                                            <  2:1552414/74‑1 (MQ=255)                             ggTCGGCGCGCTGAAGTGCAAAAAACCAGGGAGCGCGAGCTGAAGCCCGCCATCCGTAACCGCGTTAAGTCTCAgg                                          <  2:527283/76‑1 (MQ=255)                               tCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATCCGTAACCGCGTTAAGTCTCAGGCg                                        >  1:1122469/1‑76 (MQ=255)                               tCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATCCGTAACCGCGTTAAGTCTCAGGCg                                        >  2:1466895/1‑76 (MQ=255)                                  gcgcgcTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATCCGTAACCGCGATAAGTCTCAGGCGATc                                     >  2:760793/1‑76 (MQ=255)                                   cgcgcTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATCCGTAACCGCGCCAAGGCTTAGGCGATCg                                    >  2:1417411/1‑76 (MQ=255)                                       cTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAgg                                >  1:796746/1‑76 (MQ=255)                                        tGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGt                               >  2:649004/1‑76 (MQ=255)                                          aaGTGCGTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCt                             >  1:1066451/1‑76 (MQ=255)                                                gTAAAAACATGGAGCGCGAGCTGAAGCGCGCCATCCGTAACCGCGTTAAGTCTCAGGTGATCGAAGGTCTGGTaaa                       >  1:302953/1‑76 (MQ=255)                                                  aaaaaCATGGAGCGCGAGCTGAAGCCCGCCATCCGTAACCGCGTTAAGTCTCACGCGATCGAAGGTCTGGTCAAAg                     >  1:1468656/1‑76 (MQ=255)                                                                 caGCTGAAGCGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACg      >  1:225228/2‑76 (MQ=255)                                                                    cTGAAGCGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTAc   >  2:560186/1‑76 (MQ=255)                                                                     tGAAGCGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTAcc  >  1:386124/1‑76 (MQ=255)                                                                          |                                                                       TTTCGGCGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACC  >  REL606/426602‑426745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑