| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,928,593 | A→T | N126I (AAC→ATC) | rho → | transcription termination factor Rho |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,928,593 | 0 | A | T | 93.6% | 160.4 / 0.1 | 47 | N126I (AAC→ATC) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base T (24/20); total (26/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGG > REL606/3928521‑3928665 | gcccgccGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGc > 1:139173/1‑76 (MQ=255) cccgccGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGcc > 1:587338/1‑76 (MQ=255) gccgAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGc < 1:657926/76‑1 (MQ=255) cgAAAGAAGGTAAACGCTATTTTGCGCTGCTGAAAGGTAACGAAGTTAACTTCGACAAACAAAAAATCGCCCGcaa < 2:1366485/76‑1 (MQ=255) gAAAGAAGGTGAACGTTATTTTGCGCTGCTAAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGcaac < 1:1400705/76‑1 (MQ=255) aagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAAt < 1:234555/76‑1 (MQ=255) agGTGAACGCTATTTTGCGCTGCTGAAAGTTAACAAATTAACCTTCGACAAACCTGAAATCGCCCGCAACAAAATc < 2:932633/76‑1 (MQ=255) aCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCttt < 2:1316398/76‑1 (MQ=255) cGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTg < 1:1366599/76‑1 (MQ=255) cGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTg > 1:1556822/1‑76 (MQ=255) cTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTgag > 1:262021/1‑76 (MQ=255) cTATTTTGCGCTGCTAAAAGTTAACGAATTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTgag < 1:380663/76‑1 (MQ=255) tATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTgaga > 1:1232215/1‑76 (MQ=255) aTTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGaa > 2:344805/1‑76 (MQ=255) aTTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGaa > 2:517059/1‑76 (MQ=255) aTTTTGCGCTGATGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGaa > 1:1307249/1‑76 (MQ=255) aTTTTGAGCTGCTGAAAGTTAACGAGGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGaa < 1:103603/76‑1 (MQ=255) ttttGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAAc > 2:985344/1‑76 (MQ=255) ttGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACtt < 2:1020826/76‑1 (MQ=255) gcgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTaa < 1:432133/76‑1 (MQ=255) cgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAAc > 1:524800/1‑76 (MQ=255) cgcTGCTGAAAGTAAACGAATTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAAc < 2:759948/76‑1 (MQ=255) gctgctGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAAcc > 1:1688926/1‑76 (MQ=255) gctgctCAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAAcc > 2:908686/1‑76 (MQ=255) ctgctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAAccc > 1:803132/1‑76 (MQ=255) tgctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAAcccc < 2:1714402/76‑1 (MQ=255) gctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCg > 1:909576/1‑76 (MQ=255) gctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCg > 1:909595/1‑76 (MQ=255) gctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCg > 1:131582/1‑76 (MQ=255) gctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCg > 2:1624107/1‑76 (MQ=255) tgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCt > 2:422826/1‑76 (MQ=255) gAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTg > 2:202719/1‑76 (MQ=255) tAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa > 2:971673/1‑76 (MQ=255) tAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa > 1:1745897/1‑76 (MQ=255) cGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAc > 1:1558641/1‑76 (MQ=255) aaGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAActc < 2:275650/76‑1 (MQ=255) aaGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAActc > 2:430257/1‑76 (MQ=255) gTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACtctc < 1:76160/76‑1 (MQ=255) gTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACtctc > 2:1702843/1‑76 (MQ=255) cTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTg < 1:1310433/76‑1 (MQ=255) cTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTg < 2:1053783/76‑1 (MQ=255) ttCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGc < 2:1073674/76‑1 (MQ=255) ttCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGc > 2:1052025/1‑76 (MQ=255) tCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCg < 1:440310/76‑1 (MQ=255) cAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATg < 1:1654576/76‑1 (MQ=255) aCCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGaa < 1:908686/76‑1 (MQ=255) gAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTg > 2:572234/1‑76 (MQ=255) aaatCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTgg > 2:59527/1‑76 (MQ=255) | GCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGG > REL606/3928521‑3928665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |