| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,138,988 | C→T | A213V (GCG→GTG) | oxyR → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,138,988 | 0 | C | T | 96.2% | 187.0 / ‑0.9 | 54 | A213V (GCG→GTG) | oxyR | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (23/28); total (25/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01 | |||||||||||
AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATC > REL606/4138917‑4139061 | aaaaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGAt < 2:529073/76‑1 (MQ=255) aCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGaa < 1:433456/76‑1 (MQ=255) tgctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGa < 1:876056/76‑1 (MQ=255) gctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGAt < 2:229339/76‑1 (MQ=255) ctgATTGTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATa < 1:1393281/76‑1 (MQ=255) ctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATa > 2:1523731/1‑76 (MQ=255) ctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATa < 1:840022/76‑1 (MQ=255) aTGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTAGATGAAGATacac < 1:1164634/76‑1 (MQ=255) tGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATacacn < 1:57148/76‑2 (MQ=255) tGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACtt < 1:786166/76‑1 (MQ=255) ggAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTc < 2:1241850/76‑1 (MQ=255) ggAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCAGGGTGGATGAAGATACACACTTc < 2:1392131/76‑1 (MQ=255) aaGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCcg < 2:869991/76‑1 (MQ=255) gATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCcgcg < 2:1490926/76‑1 (MQ=255) tGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCcgcgcg < 1:638712/76‑1 (MQ=255) tCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGAcc < 1:1511104/76‑1 (MQ=255) tCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGAcc < 2:1041109/76‑1 (MQ=255) cagtGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACAGACTTCAGCGCGACCa > 2:1130443/4‑76 (MQ=255) aCTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAg < 2:969170/76‑1 (MQ=255) tGTTTGCGCGATCAGGCAGTGGGATTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAgat > 2:1750266/1‑74 (MQ=255) tGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGcc > 2:43945/1‑76 (MQ=255) gTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCt > 2:1713936/1‑76 (MQ=255) gTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCt > 1:238056/1‑76 (MQ=255) cgATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAAct > 1:1704223/1‑76 (MQ=255) cgATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAAct < 2:258477/76‑1 (MQ=255) aTCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAActct < 1:1252406/76‑1 (MQ=255) tCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTg > 1:455132/1‑76 (MQ=255) cAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCaa < 2:1476893/76‑1 (MQ=255) cAATGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCaa > 2:825876/1‑76 (MQ=255) cAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCaa > 2:1689431/1‑76 (MQ=255) naTGGGTCTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAAc > 1:1146010/2‑76 (MQ=255) aaTGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAAc > 1:61981/1‑76 (MQ=255) aaTGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAAc > 1:1261008/1‑76 (MQ=255) aaTGGGTTTCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAAc > 1:1064069/1‑76 (MQ=255) gggTTTCTGTTTTGCAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtg > 1:941081/1‑76 (MQ=255) ttCTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtgg > 1:673238/1‑76 (MQ=255) cTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcg < 1:629353/76‑1 (MQ=255) cTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcg < 1:398870/76‑1 (MQ=255) cTGTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcg > 1:1492965/1‑76 (MQ=255) gTTTTGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcggc > 1:1607956/1‑76 (MQ=255) ttttGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCa > 2:626076/1‑76 (MQ=255) tttGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg < 1:483656/76‑1 (MQ=255) tttGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg < 2:1436283/76‑1 (MQ=255) tttGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg < 2:1121461/76‑1 (MQ=255) tttGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg < 1:1304842/76‑1 (MQ=255) tttGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg > 1:1165121/1‑76 (MQ=255) gttGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg < 2:745475/75‑1 (MQ=255) tgcaGCCGGGGTGGATGAAGAGACGCGCTTCCGCGCGACCAGCCTGGAAACTCTGCGCACCATGGTGGCGGCAGGt < 2:547785/73‑1 (MQ=255) tGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGt < 1:378420/76‑1 (MQ=255) tGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGt > 1:323205/1‑76 (MQ=255) tGAAGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGt < 1:1282630/76‑1 (MQ=255) tGAAGCCCGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGt > 1:1105575/1‑76 (MQ=255) aaGCCGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAg > 1:1443511/1‑76 (MQ=255) aaGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTan < 1:1305550/76‑2 (MQ=255) cGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCggg > 1:552451/1‑76 (MQ=255) cGGGGTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGCAGCggg > 1:1195986/1‑76 (MQ=255) gggTGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGGAGCGGGAt > 1:1235563/1‑76 (MQ=255) ggCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATc < 1:569930/76‑1 (MQ=255) | AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATC > REL606/4138917‑4139061 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |