| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,558,224 | G→A | intergenic (‑49/+69) | yfgK ← / ← yfgL | GTP‑binding protein EngA/protein assembly complex, lipoprotein component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,558,224 | 0 | G | A | 96.0% | 174.6 / 0.2 | 50 | intergenic (‑49/+69) | yfgK/yfgL | GTP‑binding protein EngA/protein assembly complex, lipoprotein component |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base A (23/25); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.46e-01 | |||||||||||
CGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAA > REL606/2558151‑2558296 | cGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTcacg > 2:431987/1‑76 (MQ=255) gACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCAcgt < 1:1031733/76‑1 (MQ=255) aCAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCAcgtc > 2:659084/1‑76 (MQ=255) aCAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCAcgtc < 1:1330140/76‑1 (MQ=255) cAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCAcgtcg < 1:1026229/76‑1 (MQ=255) aaGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCAcgtcgt > 1:1047612/1‑76 (MQ=255) cgcgACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCgttgt > 1:719808/1‑76 (MQ=255) cgACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCCCGCTATATTCACGTCGTTGTTa < 2:1189716/76‑1 (MQ=255) cgACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTa < 2:611622/76‑1 (MQ=255) ccACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTaaaa < 1:1395517/76‑1 (MQ=255) acaGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAAc < 1:642196/76‑1 (MQ=255) gTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGa < 1:923033/76‑1 (MQ=255) gTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGa < 2:1701688/76‑1 (MQ=255) gTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGa > 2:1621933/1‑76 (MQ=255) tACCATGTTTACAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGaaa > 2:860751/1‑76 (MQ=255) tACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGaa < 2:352997/76‑1 (MQ=255) tACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGaa < 2:576188/76‑1 (MQ=255) cATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAAc < 1:19082/76‑1 (MQ=255) aTGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACg > 1:1460100/1‑76 (MQ=255) tttAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGcc < 1:589841/76‑1 (MQ=255) tttAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGcc < 2:309470/76‑1 (MQ=255) tttAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGcc < 1:652045/76‑1 (MQ=255) ttAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGccc < 1:862808/76‑1 (MQ=255) ctAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGcccc > 1:917662/2‑76 (MQ=255) aaaGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCt > 2:182546/1‑76 (MQ=255) aaaGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCt > 2:1593067/1‑76 (MQ=255) ntCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCa > 1:704917/2‑76 (MQ=255) tCATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAg < 2:1737782/76‑1 (MQ=255) cATTTTTATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAgg > 1:1701277/1‑76 (MQ=255) ttttATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGc > 1:457576/1‑76 (MQ=255) ttATAAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCg < 2:1526304/76‑1 (MQ=255) tataAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGt > 2:85342/1‑76 (MQ=255) ataAATCATCAGACAACGCACGCTAAATCCACGTCGTTGTGAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGtt < 2:422117/76‑1 (MQ=255) taAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGttt > 1:174964/1‑76 (MQ=255) taAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGttt < 1:1560644/76‑1 (MQ=255) taAATCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACACGAAAACGGCCCCTGTCCAGGAGCCGttt < 2:330280/76‑1 (MQ=255) aaaTCATCAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGtttt < 2:384811/76‑1 (MQ=255) atcatcAGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGGCCAGGAGCCGGTTTCa > 1:1229724/1‑76 (MQ=255) tcatcaGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCaa < 2:788965/76‑1 (MQ=255) catcaGCCAACGCACGCTATATTCACGTCGTTGTAAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCaaa < 2:907272/76‑1 (MQ=255) catcaGACAACGCACGCTTGATCCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCaaa < 2:413870/76‑1 (MQ=255) catcaGACAACGCACGCTATATTCACGTCGTTGTAAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCaaa < 1:651146/76‑1 (MQ=255) atcaGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAg < 2:589436/76‑1 (MQ=255) atcaGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCCGTCCCCGCGCCGGTTTCAAAg > 2:1038033/1‑76 (MQ=255) tcaGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGt > 2:1562030/1‑76 (MQ=255) tcaGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGt > 1:298561/1‑76 (MQ=255) caGCCAACGCACGCTATATTCACGTCGTTGCTAAAAACAGAAAAACGGCCCCTGCCCAGGAGCCGTTTTCAAAGTg < 2:1080585/76‑1 (MQ=255) aGACAACGCACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGa < 2:200493/76‑1 (MQ=255) aGACAACGCACGCTATATTCAAGTCGTTGTTAAAAACAGGAAAAAGGCCCCTCTCCAGGAGCCGCTTTCAAAGTGa > 2:1228345/1‑76 (MQ=255) aGACAACACACGCTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCCCTCCCGGGGGCGGTTTTAAAgaga > 1:1102517/1‑76 (MQ=255) cAACGCACCTTATATTCACGTAGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACg < 1:1297366/76‑1 (MQ=255) acgcacgcTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGAc < 2:7517/76‑1 (MQ=255) acgcacgcTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCCGCCCAGGAGCCGCTTTCAAAGTGAACGAc > 1:22919/1‑76 (MQ=255) acgcacgcTATATTCACGTCGTTGTAAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCTTTTTCAAAGTGAACGAc < 2:630345/76‑1 (MQ=255) cacgcTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACaga < 2:987463/76‑1 (MQ=255) cacgcTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACaga > 2:172255/1‑76 (MQ=255) acgcTATATTCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGGCGTTTTCAAAGTGAACGACagag > 2:1283430/1‑76 (MQ=255) atTCCCGTCGTTGTTAAAAACAGGAAAACGCCCCCTGACCCGGAGCCGTTTTCAAAGTGAACGGCAGAGCCGATTa < 2:189708/76‑1 (MQ=255) atTCACGTCGTTGTTAAAAACAGGAAAACCGCCCCTGTCCAGGAGCCCGTTTCAAAGTGAACGACAGAGAAGATTa > 1:1442248/1‑76 (MQ=255) ttCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTaa > 2:512281/1‑76 (MQ=255) ttCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTaa < 2:1469784/76‑1 (MQ=255) | CGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAA > REL606/2558151‑2558296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |