Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	4,347,649	Δ42 bp	coding (265‑306/477 nt)	fxsA →	inner membrane protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 4347648	3 (0.060)	46 (1.140)	36/120	0.1	94.5%	coding (264/477 nt)	fxsA	inner membrane protein
	?	REL606	4347691 =	3 (0.070)					coding (307/477 nt)	fxsA	inner membrane protein

GCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/4347561‑4347648 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgcttttattaccgCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGCGGCGGTTTTAGC  >  REL606/4347691‑4347765                                                                                                                                                                     GCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTG                                                                                         <  1:1628238/76‑1   AAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCT                                                                                       <  2:753297/76‑1      AAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTT                                                                                    <  1:554301/76‑1       AATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTA                                                                                   <  1:358023/76‑1          GGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTA                                                                                <  2:1207897/76‑1          GGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTA                                                                                <  2:1099644/76‑1             GGCGGCTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCG                                                                             <  2:1633327/76‑1                GGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCG                                                                          >  2:334384/1‑76                 cgaGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGG                                                                         >  2:324325/4‑76                   TGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTG                                                                       >  2:679855/1‑76                     AAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCA                                                                     >  2:1052669/1‑76                      AAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAA                                                                    >  2:506133/1‑76                         CCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAG                                                                 <  1:1379368/76‑1                           CAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCA                                                               >  1:27389/1‑76                           CAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCA                                                               >  2:728973/1‑76                               GGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTG                                                           <  1:85589/76‑1                                GCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGA                                                          <  2:1408020/76‑1                                 CGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGAC                                                         >  2:386372/1‑76                                 CGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGAC                                                         >  1:89286/1‑76                                   GAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAG                                                       <  2:960014/76‑1                                    AGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGg                                                      >  1:1342316/1‑75                                    AGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGT                                                      >  1:369837/1‑76                                      ATGATAAAAAGTGTTTCGTTGATCATTGCCGGTTTGCTGCTTTTATAACCGCCGGTGCAAAAGCATCTGACAGTGA                                                    <  1:1637392/76‑1                                          taAAAAGTGTTTCGCTGATCATTGCGGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTT                                                <  2:1318793/74‑1                                          TTAAAATTGTTTCTCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTT                                                <  2:450773/76‑1                                          TTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTT                                                <  1:241127/76‑1                                          TTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGCGAAGTT                                                >  2:1031662/1‑76                                           TAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTG                                               >  1:496985/1‑76                                             AAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGAT                                             >  2:668108/1‑76                                             AAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGCGCAAAAGCATCTGCCAGTGAAGGTGAT                                             >  2:410010/1‑76                                              AAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCACCGGTGCAAAAGCATCTGACAGTGAAGTTGATG                                            >  2:379362/1‑76                                                 gGTTTCGTTGTCCTTTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCG                                         <  2:546715/75‑1                                                  GTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGC                                        >  1:1032350/1‑76                                                  GTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGC                                        >  1:640185/1‑76                                                  GTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGC                                        <  1:164633/76‑1                                                  GTTTCGCGGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGC                                        <  2:255117/76‑1                                                     TCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATT                                     <  2:377982/76‑1                                                       GCTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTG                                   >  1:1229391/1‑76                                                        CTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGC                                  >  1:172138/1‑76                                                        CTGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGC                                  <  1:468515/76‑1                                                         TGATCATTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCG                                 >  1:1296313/1‑76                                                             ctTTGCGGGTTTTTTGATTTTATAACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTT                             <  2:945920/74‑1                                                               TTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTC                           <  1:101802/76‑1                                                               TTGCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTC                           >  2:42079/1‑76                                                                 GCCGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTC                         >  2:581148/1‑76                                                                  cgGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCG                        <  1:324325/74‑1                                                                   gGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGT                       <  1:625609/75‑1                                                                   CGGTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGT                       >  1:812008/1‑76                                                                     GTTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTAT                     <  2:511378/76‑1                                                                      TTTGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATG                    <  2:1453459/76‑1                                                                        gGCTGTTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCC                  <  2:843331/75‑1                                                                        TGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGTCAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCC                  >  2:1464615/1‑76                                                                        TGCTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCC                  <  1:1199847/76‑1                                                                          CTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCTAG                >  2:822039/1‑76                                                                          CTGCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAG                >  1:1262514/1‑76                                                                            GCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGC              <  2:1000497/76‑1                                                                            GCTTTTATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGC              >  2:632047/1‑76                                                                                 TATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGCGGCGG         >  1:805599/1‑76                                                                                 TATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGCGGCGG         <  2:1239106/76‑1                                                                                 TATTACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGCGGCGG         >  1:515456/1‑76                                                                                 TATTACCGCCGGGGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGCCGGCGG         >  1:174045/1‑76                                                                                   ttcCCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGCGGCGGTT       <  2:89286/73‑1                                                                                    TACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGCGGCGGTTT      <  1:113005/76‑1                                                                                     ACCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGCGGCGGTTTT     >  2:1391319/1‑76                                                                                      CCGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGCGGCGGTTTTA    <  2:1441509/76‑1                                                                                       CGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGCGGCGGTTTTAG   >  1:1581887/1‑76                                                                                       CGCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGCGGCGGTTTTAG   <  2:1195864/76‑1                                                                                       CGCCGGTGCAAAAGCATCTGACAGTGAAGCTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGCGGCGGTTTTAG   >  1:1581873/1‑76                                                                                        GCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGCGGCGGTTTTAGC  >  2:566174/1‑76                                                                                        GCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGCGGCGGTTTTAGC  <  2:1564826/76‑1                                                                                        GCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGCGGCGGTTTTAGC  >  2:670902/1‑76                                                                                                                                                                     GCAAAAAATGGCGGCGGGTGAAAACCCAGCGGCGGAGATGATTAAAAGTGTTTCGCTGATCATTGCCGGTTTGCTGCTTTTATTACCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/4347561‑4347648 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgcttttattaccgCCGGTGCAAAAGCATCTGACAGTGAAGTTGATGCCGCATTTGCGCTTTTCTCGTATGCCAGGCGGCGGTTTTAGC  >  REL606/4347691‑4347765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.